Magnaporthe oryzae populations adapted to finger millet and rice exhibit distinctive patterns of genetic diversity, sexuality and host interaction.

abstract：:Angiogenesis has been considered an important target for cancer therapy. The inhibition of angiogenesis represents a promising strategy for anti-cancer treatment, tumor growth inhibition, and metastasis. Vasostatin 30 (Vs30), and the 14.1 kDa vasoinhibin (Vi-II-14.1) are two peptides with remarkable anti-tumor and ant...

journal_title：Molecular biotechnology

authors： Vazquez Rodriguez G,Gonzalez C,De Leon Rodriguez A

更新日期：2013-07-01 00:00:00

abstract：:Hypermethylated in Cancer-1 (HIC-1) is a novel tumor suppressor plays crucial role in tumor formation through loss of function by hypermethylation. HIC-1 is known as transcriptional factor whereas little known about its structure and function. Requirement felt to clone and express full coding protein and reveal variou...

journal_title：Molecular biotechnology

authors： Kumar S

更新日期：2014-11-01 00:00:00

abstract：:The Embrapa 5.1 genetically modified (GM) common bean was approved for commercialization in Brazil. Methods for the quantification of this new genetically modified organism (GMO) are necessary. The development of a suitable endogenous reference is essential for GMO quantification by real-time PCR. Based on this, a new...

journal_title：Molecular biotechnology

authors： Venturelli GL,Brod FC,Rossi GB,Zimmermann NF,Oliveira JP,Faria JC,Arisi AC

更新日期：2014-11-01 00:00:00

abstract：:Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: int...

journal_title：Molecular biotechnology

authors： Cichorz S,Gośka M,Litwiniec A

更新日期：2014-10-01 00:00:00

abstract：:Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but ...

journal_title：Molecular biotechnology

authors： Yuan YJ,Ma ZY,Wu JC

更新日期：2002-02-01 00:00:00

abstract：:Compounds based on a flavonoid (di-phenolic) ring structure are emerging as a potentially important new class of pharmaceutical compounds with a broad range of biological activities, most prominent of which are their potential role as anticancer agents. These compounds exert a wide range of upregulating and downregula...

journal_title：Molecular biotechnology

authors： Brown DM,Kelly GE,Husband AJ

更新日期：2005-07-01 00:00:00

abstract：:The low economic profits of feather recycling lead that the large amount of feathers is currently discarded in China. To convert feather hydrolysates into GSH with high values, surface display of the bifunctional glutathione synthetase encoded by gcsgs from Streptococcus thermophilus on Saccharomyces cerevisiae and th...

journal_title：Molecular biotechnology

authors： Qiu Z,Tan H,Zhou S,Cao L

更新日期：2014-08-01 00:00:00

abstract：:We describe an expression system for high-yield production of recombinant soluble human FasL (rsh- FasL) in CHO cells. After one round of selection for gene amplification, cell lines producing rsh-FasL up to 60 microg/L x 10(6) cells in 24 h were obtained. Cell lines were grown in protein-free medium as suspension cul...

journal_title：Molecular biotechnology

authors： Zappitelli S,D'Alatri L,Ciucci A,Raucci G,Faiella A,Gabrielli M,Parlani M,Bressan A,Maggi CA,Goso C,Rotondaro L

更新日期：2003-03-01 00:00:00

abstract：:Tall fescue plants are widely exposed to white grubs. Regarding the rate of damage caused by the white grubs to tall fescue and difficulty of its ecological and economical control, production of resistant cultivars is a priority. In this experiment, for the first time, we report production of transgenic lines resistan...

journal_title：Molecular biotechnology

authors： Hosseini HR,Salehi H,Alichi M

更新日期：2019-07-01 00:00:00

abstract：:A single-stage polymerase-based procedure is described that allows extensive modifications of DNA. The version described here uses the QuikChange Site-Directed Mutagenesis System kit supplied by Stratagene. The original protocol is replaced by a single-stage method in which linear production of complementary strands i...

journal_title：Molecular biotechnology

authors： Salerno JC,Jones RJ,Erdogan E,Smith SM

更新日期：2005-03-01 00:00:00

abstract：:Human adipose tissue-derived stromal cells (ADSCs) are being evaluated for cardiovascular repair. We developed an ex vivo method for producing angiogenic ADSCs transduced with a self-inactivating lentiviral vector (LV) expressing the enhanced green fluorescence protein (EGFP) from an internal cytomegalovirus (CMV) pro...

journal_title：Molecular biotechnology

authors： Madonna R,Bolli R,Rokosh G,De Caterina R

更新日期：2013-05-01 00:00:00

abstract：:The two asparagine-linked glycosylation sites of recombinant coagulation factor VIIa have been characterized by glycosidase digestions, size-exclusion chromatography (SEC), and mass spectrometry (MS). Nine structures were characterized as core fucosylated bi-and triantennary structures with 0-3 sialic-acid residues wh...

journal_title：Molecular biotechnology

authors： Klausen NK,Bayne S,Palm L

更新日期：1998-06-01 00:00:00

abstract：:A short (43-bp) A/T-rich stretch of DNA located in the intergenic region between the baiA2 and baiF genes from Eubacterium sp. strain VPI 12708 was amplified by polymerase chain reaction (PCR) and inserted in front of the Shine-Dalgarno (SD) sequences of three inefficiently-expressed Eubacterium sp. strain VPI 12708 g...

journal_title：Molecular biotechnology

authors： Mallonee DH,Hylemon PB

更新日期：1999-02-01 00:00:00

abstract：:Jet-injection has become an applicable technology among other established nonviral delivery systems, such as particle bombardment or in vivo electroporation. The low-volume jet injector employed in this study uses compressed air to inject solutions of 1.5-10 microL containing naked DNA into the desired tissue. The nov...

journal_title：Molecular biotechnology

authors： Walther W,Stein U,Fichtner I,Voss C,Schmidt T,Schleef M,Nellessen T,Schlag PM

更新日期：2002-06-01 00:00:00

abstract：:Virus crystallography can provide atomic resolution structures for intact isometric virus particles and components thereof. The methodology is illustrated by reference to a particularly complex example, the core of the bluetongue virus (700 A). ...

journal_title：Molecular biotechnology

authors： Fry EE,Grimes J,Stuart DI

更新日期：1999-08-01 00:00:00

abstract：:Considering the fact that Prays oleae is one of the most pathogenic insects to the olive tree in the Mediterranean basin, particularly in Tunisia, the mode of action of Cry insecticidal toxins of Bacillus thuringiensis kurstaki in Prays oleae midgut was investigated. The proteolysis of Bacillus thuringiensis delta-end...

journal_title：Molecular biotechnology

authors： Rouis S,Chakroun M,Saadaoui I,Jaoua S

更新日期：2007-02-01 00:00:00

abstract：:In an effort to develop microalgae as a robust system for the production of valuable proteins, we analyzed some of the factors affecting recombinant protein expression in the chloroplast of the green alga Chlamydomonas reinhardtii. We monitored mRNA accumulation, protein synthesis, and protein turnover for three codon...

journal_title：Molecular biotechnology

authors： Coragliotti AT,Beligni MV,Franklin SE,Mayfield SP

更新日期：2011-05-01 00:00:00

abstract：:A total of 41 accessions of Aglianico belonging to three different biotypes (Taburno, Taurasi, and Vulture) and 9 accessions of Sirica grapes were sampled from diverse areas of Campania (Italy). All accessions were first genotyped using 21 microsatellite markers (SSR) to evaluate possible homonymies, synonymies, and t...

journal_title：Molecular biotechnology

authors： De Lorenzis G,Imazio S,Biagini B,Failla O,Scienza A

更新日期：2013-06-01 00:00:00

abstract：:A bacterial artificial chromosome library of the causal agent of the Black Sigatoka leaf spot disease of banana and plantain, Mycosphaerella fijiensis, has been constructed using a non-sphaeroplasting technique and characterized using both homologous and heterologous probes. After first and a second size selection of ...

journal_title：Molecular biotechnology

authors： Canto-Canché B,Guillén-Maldonado DK,Peraza-Echeverría L,Conde-Ferráez L,James-Kay A

更新日期：2007-05-01 00:00:00

abstract：:Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...

journal_title：Molecular biotechnology

authors： Shamriz S,Ofoghi H

更新日期：2019-02-01 00:00:00

abstract：:Derivation of cardiomyocytes directly from patients' own fibroblasts could offer a new therapeutic approach for those with ischemic heart disease. An essential step toward clinical application is to establish safe conversion of human fibroblasts into a cardiac fate. Here we aimed to efficiently and safely generate car...

journal_title：Molecular biotechnology

authors： Ghazizadeh Z,Rassouli H,Fonoudi H,Alikhani M,Talkhabi M,Darbandi-Azar A,Chen S,Baharvand H,Aghdami N,Salekdeh GH

更新日期：2017-06-01 00:00:00

abstract：:Virus-like particles (VLPs) have vast potential for applications in nanoscience and nanomedicine. These biological nanoparticles may be used for medical imaging, vaccination, or tissue-specific delivery of drugs or other bioactive molecules. VLPs of Human parvovirus B19 (B19 V) can be assembled in vitro from the recom...

journal_title：Molecular biotechnology

authors： Sánchez-Rodríguez SP,Enrriquez-Avila JV,Soto-Fajardo JM,Peña-Montes C,Bustos-Jaimes I

更新日期：2015-04-01 00:00:00

abstract：:Human immunodeficiency virus types 1 and 2 (HIV-1, -2), the etiological agents of AIDS, are retroviruses that replicate in CD4+ T lymphocytes, monocytes, and macrophages. Early anti-HIV therapies were directed at the step in the virus life cycle that was considered the most readily blocked: the transcription of viral ...

journal_title：Molecular biotechnology

authors： Patience C,Moore J,Boyd M

更新日期：1994-02-01 00:00:00

abstract：:Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...

journal_title：Molecular biotechnology

authors： Noble JE,Knight AE,Reason AJ,Di Matola A,Bailey MJ

更新日期：2007-10-01 00:00:00

abstract：:We investigated the efficiency of a single plasmid transfection along the longitudinal axis of the regenerating soleus of young rats. This also reflected transfection efficiency along the fibers because the soleus is a nearly fusiform muscle in young animals. The complete regeneration was induced by notexin and the tr...

journal_title：Molecular biotechnology

authors： Kósa M,Zádor E

更新日期：2013-06-01 00:00:00

abstract：:To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most pro...

journal_title：Molecular biotechnology

authors： Gach JS,Quendler H,Ferko B,Katinger H,Kunert R

更新日期：2008-06-01 00:00:00

abstract：:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...

journal_title：Molecular biotechnology

authors： Kalwy S,Rance J,Young R

更新日期：2006-10-01 00:00:00

abstract：:ATP-binding cassette transporter G2 (ABCG2) gene encodes a protein that has a wide variety of substrates and is responsible for the active secretion of clinically and toxicologically important molecules into milk. Although known in many species, this marks the first time this gene product has been reported in goats. I...

journal_title：Molecular biotechnology

authors： Wu HJ,Luo J,Wu N,Matand K,Zhang LJ,Han XF,Yang BJ

更新日期：2008-05-01 00:00:00

abstract：:Recent human clinical trials results demonstrated successful treatment for certain genetic forms of cystic fibrosis (CF). To extend treatment opportunities to those afflicted with other genetic forms of CF disease, structural and biophysical characterization of CF transmembrane conductance regulator (CFTR) is urgently...

journal_title：Molecular biotechnology

authors： Hildebrandt E,Mulky A,Ding H,Dai Q,Aleksandrov AA,Bajrami B,Diego PA,Wu X,Ray M,Naren AP,Riordan JR,Yao X,DeLucas LJ,Urbatsch IL,Kappes JC

更新日期：2015-05-01 00:00:00

abstract：:Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...

journal_title：Molecular biotechnology

authors： Samuel RVM,Farrukh SY,Rehmat S,Hanif MU,Ahmed SS,Musharraf SG,Durrani FG,Saleem M,Gul R

更新日期：2018-08-01 00:00:00