Abstract:
:To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most prominent neutralizing antibodies. Anti-idiotypic antibody Ab2/3H6 has been partially humanized and expressed as whole immunoglobulin G in Chinese hamster ovary cells in order to minimize the human anti-mouse antibody response. Here we describe the expression, purification, and immunohistochemical characterization of the chimeric Ab2/3H6 Fab fragment, which was finally used beside the whole IgG1 as an antigen for immunization of guinea pigs. The crude sera were screened for specific antibodies against the epitope of mAb 2F5 ELDKWA as well as for reactivity against HIV-1 gp41.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Gach JS,Quendler H,Ferko B,Katinger H,Kunert Rdoi
10.1007/s12033-008-9054-7subject
Has Abstractpub_date
2008-06-01 00:00:00pages
119-25issue
2eissn
1073-6085issn
1559-0305journal_volume
39pub_type
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