Abstract:
:Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on shoots of young maize seedlings after cocultivation with Agrobacterium.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Shen WH,Escudero J,Hohn Bdoi
10.1385/MB:13:2:165keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
165-70issue
2eissn
1073-6085issn
1559-0305pii
MB:13:2:165journal_volume
13pub_type
杂志文章abstract::Human adipose tissue-derived stromal cells (ADSCs) are being evaluated for cardiovascular repair. We developed an ex vivo method for producing angiogenic ADSCs transduced with a self-inactivating lentiviral vector (LV) expressing the enhanced green fluorescence protein (EGFP) from an internal cytomegalovirus (CMV) pro...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9537-4
更新日期:2013-05-01 00:00:00
abstract::Human papillomavirus (HPV) is the causal agent of cervical cancer, one of the most common causes of death in women worldwide, and its E7 antigen is the major candidate for a therapeutic vaccine. The large scale production of E7 by molecular farming that would lead to the development of a safe and inexpensive vaccine i...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9781-x
更新日期:2014-11-01 00:00:00
abstract::Indirubin is a strong inhibitor of several eukaryotic cell signaling pathways and shows promise as a treatment for myelocytic leukemia and Alzheimer's disease. The tmoABCDEF operon, encoding the components of a novel toluene 4-monooxygenase from the paint factory soil isolate, Pseudomonas sp. M4, was cloned and expres...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9863-4
更新日期:2015-08-01 00:00:00
abstract::To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most pro...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9054-7
更新日期:2008-06-01 00:00:00
abstract::CD151 is an abundantly expressed eukaryotic transmembrane protein on the cell surface. It is involved in cell adhesion, angiogenesis and signal transduction as well in disease conditions such as cancer and viral infections. However, the molecular mechanism of CD151 activation is poorly understood due to the lack of st...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00212-3
更新日期:2019-12-01 00:00:00
abstract::In this study, human alpha-1,4-N-acetylglucosaminyltransferase (alpha4GnT) fused with GFP(uv) (GFP(uv)-alpha4GnT) was expressed using both a transformed cell system and silkworm larvae. A Tn-pXgp-GFP(uv)-alpha4GnT cell line, isolated after expression vector transfection, produced 106 mU/ml of alpha4GnT activity in sus...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9174-8
更新日期:2009-09-01 00:00:00
abstract::Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency of gRNA need to be developed. Three gRNAs with ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00290-8
更新日期:2021-02-01 00:00:00
abstract::Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-007-0038-9
更新日期:2007-10-01 00:00:00
abstract::A duplex real-time PCR assay with melting curve analysis, using the EvaGreen fluorescence dye, was developed for rapid and reliable identification of bovine and caprine in ruminant feeds. The method merges the use of bovine (Bos taurus) and caprine (Capra hircus) specific primers that amplify small fragments (bovine 9...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9756-y
更新日期:2014-08-01 00:00:00
abstract::L-Cysteine desulfhydrase (DES; EC 4.4.1.1) is the most important enzyme that catalyzes the decomposition of L-cysteine to pyruvate, ammonia, and hydrogen sulfide (H2S), the latter of which has recently been recognized as the third gasotransmitter for multiple signaling events in plants. Previous results showed the exi...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9621-9
更新日期:2013-07-01 00:00:00
abstract::GM risk assessments (GMRAs) play an important role in the decision-making process surrounding the regulation, notification and permission to handle Genetically Modified Organisms (GMOs). Ultimately the role of each GMRA will be able to ensure the safe handling and containment of the GMO; and to asses any potential imp...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-009-9237-x
更新日期:2010-03-01 00:00:00
abstract::Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadv...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:17:3:219
更新日期:2001-03-01 00:00:00
abstract::The GacS/A system in Azotobacter vinelandii regulates alginate and alkylresorcinols production through RsmZ1, a small regulatory RNA (sRNA) that releases the translational repression of the algD and arpR mRNAs caused by the RsmA protein. In the Pseudomonadaceae family, the Rsm-sRNAs are grouped into three families: Rs...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0102-7
更新日期:2018-09-01 00:00:00
abstract::Artificial microRNA (amiRNA) has become a powerful tool for gene silencing in plants. A new method for easy and rapid construction of rice artificial miRNA vector is described. The procedure involved modification of the pCAMBIA1300-UR vector by insertion of a 'vector modification fragment'. This was prepared from the ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9291-4
更新日期:2010-11-01 00:00:00
abstract::De novo design provides an in silico toolkit for the design of novel small molecular structures to a set of specified structural constraints. With the avalanche of bioinformatics data, de novo design is ideally suited for exploring molecules that could be useful for chemical genomics. The design process involves manip...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-007-0037-x
更新日期:2007-11-01 00:00:00
abstract::The electron microscopic study of DNA-protein complexes can yield valuable information that is often not easily available by other methods. In this article we give a number of examples that were chosen to illustrate the utility of electron microscopy. Along with the strategy used are protocols that allow such experime...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:16:1:77
更新日期:2000-09-01 00:00:00
abstract::A new lipase gene designated as SlLipA was isolated from Serratia liquefaciens S33 DB-1 by the genomic-walking method. The cloned gene contained an open reading frame (ORF) of 1,845 bp encoding 615 amino acids with a conserved GXSXG motif. Genome sequence analysis showed that an aldo/keto reductase gene closed to the ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-007-9007-6
更新日期:2008-02-01 00:00:00
abstract::Menstrual blood has been recognized as an easily accessible and inexpensive source of stem cells, in recent years. To establish a safe and efficient protocol for development of menstrual blood-derived stem cells (MenSCs) into osteoblasts, the effect of substitution of fetal bovine serum (FBS) with human platelet deriv...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9698-9
更新日期:2014-03-01 00:00:00
abstract::Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9531-x
更新日期:2013-03-01 00:00:00
abstract::Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to oth...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9384-8
更新日期:2011-10-01 00:00:00
abstract::Immobilized-metal-ion affinity chromatography (IMAC) is used extensively for phosphopeptide enrichment in phosphoproteomics. However, the effect of nucleic acids in protein samples on phosphopeptide enrichment by IMAC has not yet been well clarified. In this study, we demonstrate that IMAC beads possess a strong adsor...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9176-6
更新日期:2009-09-01 00:00:00
abstract::HOX genes are highly conserved transcription factors that determine the identity of cells and tissues along the anterior-posterior body axis in developing embryos. Aberrations in HOX gene expression have been shown in various tumors. However, the correlation of HOX gene expression patterns with tumorigenesis and cance...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9682-4
更新日期:2014-01-01 00:00:00
abstract::This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9365-3
更新日期:2011-07-01 00:00:00
abstract::Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789104
更新日期:1995-04-01 00:00:00
abstract::Enterotoxigenic strains of Escherichia coli produce a heat-labile holotoxin (LT), which causes diarrhea. We engineered corn seeds to produce LT-B, the nontoxic subunit of LT, to serve as a plant-derived vaccine to traveler's diarrhea and as an adjuvant for co-administered proteins. We previously demonstrated that a st...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:32:1:031
更新日期:2006-01-01 00:00:00
abstract::In an effort to develop microalgae as a robust system for the production of valuable proteins, we analyzed some of the factors affecting recombinant protein expression in the chloroplast of the green alga Chlamydomonas reinhardtii. We monitored mRNA accumulation, protein synthesis, and protein turnover for three codon...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9348-4
更新日期:2011-05-01 00:00:00
abstract::Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ab...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9850-9
更新日期:2015-06-01 00:00:00
abstract::Virus crystallography can provide atomic resolution structures for intact isometric virus particles and components thereof. The methodology is illustrated by reference to a particularly complex example, the core of the bluetongue virus (700 A). ...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:12:1:13
更新日期:1999-08-01 00:00:00
abstract::In this study, we investigated the relative expression of the Rous sarcoma virus (RSV) promoter-driven expression of enhanced green fluorescent protein (EGFP) in fibroblasts of transgenic quails. We analyzed the direct influence of CpG methylation of the RSV promoter on the transcriptional activity of delivered transg...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9393-7
更新日期:2011-11-01 00:00:00
abstract::Over the last decade transgenic mouse models have become a common experimental tool for unraveling gene function. During this time there has been a growing expectation that transgenes resemble the in vivo state as much as possible. To this end, a preference away from heterologous promoters has emerged, and transgene c...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:29:2:153
更新日期:2005-02-01 00:00:00