Abstract:
:Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to other methods tested, effectively purify high quality nucleic acids from plant tissues rich in polysaccharides and polyphenolic compounds such as different grape tissues and fruit tissue of fruit trees. The nucleic acids isolated with this protocol were successfully used for many functional genomic based experiments including polymerase chain reaction, reverse transcription polymerase chain reaction (RT-PCR), cloning, and semiquantitative RT-PCR.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Japelaghi RH,Haddad R,Garoosi GAdoi
10.1007/s12033-011-9384-8subject
Has Abstractpub_date
2011-10-01 00:00:00pages
129-37issue
2eissn
1073-6085issn
1559-0305journal_volume
49pub_type
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