Adenovirus Dodecahedron, a VLP, Can be Purified by Size Exclusion Chromatography Instead of Time-Consuming Sucrose Density Gradient Centrifugation.

abstract：:CD151 is an abundantly expressed eukaryotic transmembrane protein on the cell surface. It is involved in cell adhesion, angiogenesis and signal transduction as well in disease conditions such as cancer and viral infections. However, the molecular mechanism of CD151 activation is poorly understood due to the lack of st...

journal_title：Molecular biotechnology

authors： Purushothaman G,Thiruvenkatam V

更新日期：2019-12-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field ...

journal_title：Molecular biotechnology

authors： Jurinke C,Oeth P,van den Boom D

更新日期：2004-02-01 00:00:00

abstract：:Biobanks, more formally known as biological resource centers (BRCs), form an "unsung" yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and cell-lines would be effectively impossible. BRCs...

journal_title：Molecular biotechnology

authors： Day JG,Stacey GN

更新日期：2008-10-01 00:00:00

abstract：:Megaprimer-based methodology has been widely applied in site-directed mutagenesis, but rarely used in gene splicing. In this article, we describe a modification of the megaprimer PCR method, which can efficiently create and amplify a specific ligated chimeric gene segment in a PCR reaction and under a common PCR progr...

journal_title：Molecular biotechnology

authors： Chen JR,Lü JJ,Wang HF

更新日期：2008-11-01 00:00:00

abstract：:Artificial microRNA (amiRNA) has become a powerful tool for gene silencing in plants. A new method for easy and rapid construction of rice artificial miRNA vector is described. The procedure involved modification of the pCAMBIA1300-UR vector by insertion of a 'vector modification fragment'. This was prepared from the ...

journal_title：Molecular biotechnology

authors： Wang X,Yang Y,Yu C,Zhou J,Cheng Y,Yan C,Chen J

更新日期：2010-11-01 00:00:00

abstract：:Over the last decade transgenic mouse models have become a common experimental tool for unraveling gene function. During this time there has been a growing expectation that transgenes resemble the in vivo state as much as possible. To this end, a preference away from heterologous promoters has emerged, and transgene c...

journal_title：Molecular biotechnology

authors： Ristevski S

更新日期：2005-02-01 00:00:00

abstract：:Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utiliz...

journal_title：Molecular biotechnology

authors： Wang X,Tian W,Li Y

更新日期：2008-01-01 00:00:00

abstract：:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...

journal_title：Molecular biotechnology

authors： Wang XW,Xiong AS,Yao QH,Zhang Z,Qiao YS

更新日期：2010-01-01 00:00:00

abstract：:ATP-binding cassette transporter G2 (ABCG2) gene encodes a protein that has a wide variety of substrates and is responsible for the active secretion of clinically and toxicologically important molecules into milk. Although known in many species, this marks the first time this gene product has been reported in goats. I...

journal_title：Molecular biotechnology

authors： Wu HJ,Luo J,Wu N,Matand K,Zhang LJ,Han XF,Yang BJ

更新日期：2008-05-01 00:00:00

abstract：:There is a growing pharmaceutical market for protein-based drugs for use in therapy and diagnosis. The rapid developments in molecular and cell biology have resulted in production of expression systems for manufacturing of recombinant proteins and monoclonal antibodies. These proteins are glycosylated when expressed i...

journal_title：Molecular biotechnology

authors： Nilsson B

更新日期：1994-12-01 00:00:00

abstract：:The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes needed for efficient solubilization of native cellulose, the major component of plants. In this work, we reported the molecular characterization of an important glycosyl-hydrolase enzyme classified as endo-β-1,4-glucanase. ...

journal_title：Molecular biotechnology

authors： Chahed H,Ezzine A,Mlouka AB,Hardouin J,Jouenne T,Marzouki MN

更新日期：2014-04-01 00:00:00

abstract：:In the context of clinical gene transfer using viral vectors, the risk of memory antivector immunity is often poorly appreciated. The immunological past of the patient, the site of injection, and the vector dose will play intertwined and decisive roles in the safety and efficacy of treatment. To circumvent the drawbac...

journal_title：Molecular biotechnology

authors： Perreau M,Kremer EJ

更新日期：2006-10-01 00:00:00

abstract：:D-Allulose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity. Using D-psicose epimerase and L-rhamnulose kinase, a method enabling high conversio...

journal_title：Molecular biotechnology

authors： Xiao Q,Niu J,Liu H,Liu Y,Zhou X

更新日期：2019-06-01 00:00:00

abstract：:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...

journal_title：Molecular biotechnology

authors： Ryu KH,Choi SH,Lee JS

更新日期：2000-01-01 00:00:00

abstract：:A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...

journal_title：Molecular biotechnology

authors： Kapros T,Robertson AJ,Waterborg JH

更新日期：1994-08-01 00:00:00

abstract：:Pasteurella multocida is the main cause of haemorrhagic septicaemia (HS) outbreak in livestock, such as cattle and buffaloes. Conventional vaccines such as alum-precipitated or oil-adjuvant broth bacterins were injected subcutaneously to provide protection against HS. However, the immunity developed is only for short ...

journal_title：Molecular biotechnology

authors： Chelliah S,Velappan RD,Lim KT,Swee CWK,Nor Rashid N,Rothan HA,Kabir N,Ismail S

更新日期：2020-05-01 00:00:00

abstract：:IMGT, the International ImMunoGeneTics information system ( http://imgt.cines.fr ), was created in 1989 by the Laboratoire d'ImmunoGénétique Moléculaire (LIGM) (Université Montpellier 2 and CNRS) at Montpellier, France, in order to standardize and manage the complexity of immunogenetics data. IMGT is recognized as the...

journal_title：Molecular biotechnology

authors： Lefranc MP

更新日期：2008-09-01 00:00:00

abstract：:The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of d...

journal_title：Molecular biotechnology

authors： Thanh T,Omar H,Abdullah MP,Chi VT,Noroozi M,Ky H,Napis S

更新日期：2009-10-01 00:00:00

abstract：:This article reviews some of the issues involved in evaluating vaccines in humans. Vaccine trials are required for licensure and are essential for demonstrating a vaccine's safety and protective efficacy. The formal framework of phase I, II, and III trials is described, with particular emphasis on the choice of hypoth...

journal_title：Molecular biotechnology

authors： Farrington CP,Miller E

更新日期：2001-01-01 00:00:00

abstract：:Sample preparation is crucial for obtaining high-quality proteins for the purpose of electrophoretic separation and further analysis from tissues that contain high levels of interfering compounds. Hypericum perforatum is a medicinal plant that contains high amounts of phenolic compounds, of which hypericins, hyperfori...

journal_title：Molecular biotechnology

authors： Karppinen K,Taulavuori E,Hohtola A

更新日期：2010-11-01 00:00:00

abstract：:Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...

journal_title：Molecular biotechnology

authors： Pegoraro M,Matić S,Pergolizzi B,Iannarelli L,Rossi AM,Morra M,Noris E

更新日期：2017-10-01 00:00:00

abstract：:G-quartets are square planar arrangements of four guanine bases, which can form extraordinarily stable stacks when present in nucleic acid sequences. Such G-quadruplex structures were long regarded as an in vitro phenomenon, but the widespread presence of suitable sequences in genomes and the identification of protein...

journal_title：Molecular biotechnology

authors： Bryan TM,Baumann P

更新日期：2011-10-01 00:00:00

abstract：:Yeast artificial chromosome (YAC) cloning systems enable the cloning of DNA stretches of 50 to well over 2000 kb. This makes it possible to study large intact regions of DNA in detail, by restriction mapping the YAC to produce a physical map and by examining the YAC for coding sequences or genes. YACs are important fo...

journal_title：Molecular biotechnology

authors： Ramsay M

更新日期：1994-04-01 00:00:00

abstract：:Compounds based on a flavonoid (di-phenolic) ring structure are emerging as a potentially important new class of pharmaceutical compounds with a broad range of biological activities, most prominent of which are their potential role as anticancer agents. These compounds exert a wide range of upregulating and downregula...

journal_title：Molecular biotechnology

authors： Brown DM,Kelly GE,Husband AJ

更新日期：2005-07-01 00:00:00

abstract：:The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...

journal_title：Molecular biotechnology

authors： Kulichkova VA,Artamonova TO,Zaykova JJ,Ermolaeva JB,Khodorkovskii MA,Barlev NA,Tomilin AN,Tsimokha AS

更新日期：2015-01-01 00:00:00

abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...

journal_title：Molecular biotechnology

authors： do Nascimento NC,Menguer PK,Sperotto RA,de Almeida MR,Fett-Neto AG

更新日期：2013-05-01 00:00:00

abstract：:The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...

journal_title：Molecular biotechnology

authors： Isik N,White L,Barnes R,Poynton CJ,Mills KI

更新日期：2003-07-01 00:00:00

abstract：:DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation...

journal_title：Molecular biotechnology

authors： Briddon RW,Bull SE,Mansoor S,Amin I,Markham PG

更新日期：2002-03-01 00:00:00

abstract：:Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...

journal_title：Molecular biotechnology

authors： Madonna R,Delli Pizzi S,Tartaro A,De Caterina R

更新日期：2014-05-01 00:00:00

abstract：:To develop combined vaccine systems, we have generated Edwardsiella tarda ghosts (ETG) displaying a foreign protein on the outer membrane and also Ed. tarda cadaver (ETC) expressing a heterologous protein in the cytoplasm. Green fluorescent protein (GFP) was used as a model foreign protein. A constitutive promoter (Et...

journal_title：Molecular biotechnology

authors： Choi SH,Nam YK,Kim KH

更新日期：2010-10-01 00:00:00