Abstract:
:Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field of nucleic acid analysis. These include the analysis of mutations, the resequencing of amplicons with a known reference sequence, and the quantitative analysis of gene expression and allelic frequencies in complex DNA mixtures.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Jurinke C,Oeth P,van den Boom Ddoi
10.1385/MB:26:2:147keywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
147-64issue
2eissn
1073-6085issn
1559-0305pii
MB:26:2:147journal_volume
26pub_type
杂志文章,评审abstract::An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could repr...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9516-9
更新日期:2013-01-01 00:00:00
abstract::Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:34:2:151
更新日期:2006-10-01 00:00:00
abstract::The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticu...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9486-3
更新日期:2012-10-01 00:00:00
abstract::This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9365-3
更新日期:2011-07-01 00:00:00
abstract::Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0065-8
更新日期:2018-03-01 00:00:00
abstract::Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9531-x
更新日期:2013-03-01 00:00:00
abstract::Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789331
更新日期:1995-06-01 00:00:00
abstract::The major online single nucleotide polymorphism (SNP) databases freely available as research tools for genetic analysis are explained, reviewed, and compared. An outline is given of the search strategies that can be used with the most extensive current SNP databases: National Centre for Biotechnology Information (NCBI...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/mb:35:1:65
更新日期:2007-01-01 00:00:00
abstract::Pasteurella multocida is the main cause of haemorrhagic septicaemia (HS) outbreak in livestock, such as cattle and buffaloes. Conventional vaccines such as alum-precipitated or oil-adjuvant broth bacterins were injected subcutaneously to provide protection against HS. However, the immunity developed is only for short ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00244-0
更新日期:2020-05-01 00:00:00
abstract::Since it was first introduced in late 1990s Wave bioreactor has been used for protein production by mammalian and insect cell lines. However, using Wave bioreactor to produce human monoclonal antibody by stable Drosophila Schneider 2 (S2) cell transfectants has not been reported before. In this study, S2 cells were co...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9484-5
更新日期:2012-10-01 00:00:00
abstract::We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9260-y
更新日期:2010-06-01 00:00:00
abstract::Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last y...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-011-9402-x
更新日期:2011-11-01 00:00:00
abstract::Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to oth...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9384-8
更新日期:2011-10-01 00:00:00
abstract::Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achieveme...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-018-0133-0
更新日期:2019-01-01 00:00:00
abstract::Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9204-6
更新日期:2010-01-01 00:00:00
abstract::A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789291
更新日期:1994-08-01 00:00:00
abstract::Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher's disease, the most common inherited storage disorder. Treatment consists of enzyme replacement therapy by the administration of recombinant GCR produced in Chinese hamster ovary cells. The production of anti-GCR antibodies has already been...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9303-4
更新日期:2010-11-01 00:00:00
abstract::Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02740841
更新日期:1998-12-01 00:00:00
abstract::The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes needed for efficient solubilization of native cellulose, the major component of plants. In this work, we reported the molecular characterization of an important glycosyl-hydrolase enzyme classified as endo-β-1,4-glucanase. ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9714-0
更新日期:2014-04-01 00:00:00
abstract::The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0046-3
更新日期:2018-01-01 00:00:00
abstract::As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:14:3:197
更新日期:2000-03-01 00:00:00
abstract::The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9799-0
更新日期:2015-01-01 00:00:00
abstract::Previous studies have shown that mRNA-electroporated dendritic cells (DCs) are able to process and present tumor-associated antigens, leading to the activation of tumor-specific T cells in vitro and in vivo. However, the optimal maturation state of antigen loading and half-life of the mRNA-translated protein product a...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9071-6
更新日期:2008-10-01 00:00:00
abstract::A gene encoding maltogenic amylase from acidic Bacillus sp. US149 (maUS149) was cloned, sequenced and over-expressed in Escherichia coli. The nucleotide sequence analysis revealed an open reading frame (ORF) of 1749 bp encoding a protein of 582 residues. The alignment of deduced amino acid sequence revealed a relative...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-007-9017-4
更新日期:2008-03-01 00:00:00
abstract::The low economic profits of feather recycling lead that the large amount of feathers is currently discarded in China. To convert feather hydrolysates into GSH with high values, surface display of the bifunctional glutathione synthetase encoded by gcsgs from Streptococcus thermophilus on Saccharomyces cerevisiae and th...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9750-4
更新日期:2014-08-01 00:00:00
abstract::The Embrapa 5.1 genetically modified (GM) common bean was approved for commercialization in Brazil. Methods for the quantification of this new genetically modified organism (GMO) are necessary. The development of a suitable endogenous reference is essential for GMO quantification by real-time PCR. Based on this, a new...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9786-5
更新日期:2014-11-01 00:00:00
abstract::A duplex real-time PCR assay with melting curve analysis, using the EvaGreen fluorescence dye, was developed for rapid and reliable identification of bovine and caprine in ruminant feeds. The method merges the use of bovine (Bos taurus) and caprine (Capra hircus) specific primers that amplify small fragments (bovine 9...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9756-y
更新日期:2014-08-01 00:00:00
abstract::The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those s...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:29:1:57
更新日期:2005-01-01 00:00:00
abstract::The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:24:3:229
更新日期:2003-07-01 00:00:00
abstract::Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor, that has been used as a therapeutic agent in facilitating bone marrow and stem cell transplantation and in other clinical cases like neutropenia. Although biologically active recombinant GM-CSF has been successfully produced in...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:30:2:103
更新日期:2005-06-01 00:00:00