Deciphering enzyme function using peptide arrays.

abstract：:The significance of actin cytoskeleton on cell growth was historically studied using toxic drugs, such as cytochalasin. However, it is possible that unpredictable effects of these agents may have influenced the reported observations. In our study, we have established a drug-free system using cofilin overexpression to ...

journal_title：Molecular biotechnology

authors： Lee YJ,Keng PC

更新日期：2005-09-01 00:00:00

abstract：:The induction of several secondary metabolites in plants is one of the most commonly observed effects after the external addition of methyl jasmonate (MeJA). After the elicitation of Catharanthus roseus hairy roots with different concentrations of MeJA, changes in the accumulation of alkaloids such as ajmalicine, serp...

journal_title：Molecular biotechnology

authors： Ruiz-May E,Galaz-Avalos RM,Loyola-Vargas VM

更新日期：2009-03-01 00:00:00

abstract：:Menstrual blood has been recognized as an easily accessible and inexpensive source of stem cells, in recent years. To establish a safe and efficient protocol for development of menstrual blood-derived stem cells (MenSCs) into osteoblasts, the effect of substitution of fetal bovine serum (FBS) with human platelet deriv...

journal_title：Molecular biotechnology

authors： Kazemnejad S,Najafi R,Zarnani AH,Eghtesad S

更新日期：2014-03-01 00:00:00

abstract：:A bacterial artificial chromosome library of the causal agent of the Black Sigatoka leaf spot disease of banana and plantain, Mycosphaerella fijiensis, has been constructed using a non-sphaeroplasting technique and characterized using both homologous and heterologous probes. After first and a second size selection of ...

journal_title：Molecular biotechnology

authors： Canto-Canché B,Guillén-Maldonado DK,Peraza-Echeverría L,Conde-Ferráez L,James-Kay A

更新日期：2007-05-01 00:00:00

abstract：:The diversity and complexity of bioinformatics tools currently available for protein sequence analysis can make it difficult to know where to begin when presented with a new sequence. In this article, we present a protocol outlining one approach to sequence analysis that should give as comprehensive a picture as possi...

journal_title：Molecular biotechnology

authors： Russell RB

更新日期：2007-07-01 00:00:00

abstract：:The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticu...

journal_title：Molecular biotechnology

authors： Cai H,Tian S,Dong H

更新日期：2012-10-01 00:00:00

abstract：:Host protein synthesis is shut down in the lytic baculovirus expression vector system (BEVS). This also affects host proteins involved in routing secretory proteins through the endoplasmic reticulum (ER)-Golgi system. It has been demonstrated that a secretory alkaline phosphatase-EGFP fusion protein (SEFP) can act as ...

journal_title：Molecular biotechnology

authors： Teng CY,Chang SL,van Oers MM,Wu TY

更新日期：2013-05-01 00:00:00

abstract：:Angiogenesis has been considered an important target for cancer therapy. The inhibition of angiogenesis represents a promising strategy for anti-cancer treatment, tumor growth inhibition, and metastasis. Vasostatin 30 (Vs30), and the 14.1 kDa vasoinhibin (Vi-II-14.1) are two peptides with remarkable anti-tumor and ant...

journal_title：Molecular biotechnology

authors： Vazquez Rodriguez G,Gonzalez C,De Leon Rodriguez A

更新日期：2013-07-01 00:00:00

abstract：:Tea, a beverage crop, is a rich source of polyphenols and polysaccharides which greatly attribute to its importance. However, oxidation and precipitation of these compounds during nucleic acids extraction is a limitation to molecular biology and genomic studies. On isolation of total RNA from root tissue using establi...

journal_title：Molecular biotechnology

authors： Muoki RC,Paul A,Kumari A,Singh K,Kumar S

更新日期：2012-09-01 00:00:00

abstract：:The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of d...

journal_title：Molecular biotechnology

authors： Thanh T,Omar H,Abdullah MP,Chi VT,Noroozi M,Ky H,Napis S

更新日期：2009-10-01 00:00:00

abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...

journal_title：Molecular biotechnology

authors： Novo C,Domingos A,Karmali A

更新日期：2001-02-01 00:00:00

abstract：:We report a rapid and simple method for isolating the 5'-end of plant genes from genomic DNA by polymerase chain reaction (PCR) with TATA-box-based degenerate primers (TDPs). The TDPs were specially designed according to the TATA box, which is conserved in the promoter region of most plant genes. The unknown 5'-ends o...

journal_title：Molecular biotechnology

authors： Guo Y,Ma L,Ji Y,Pu G,Liu B,Du Z,Li G,Ye H,Wang H

更新日期：2011-02-01 00:00:00

abstract：:In this review I briefly describe the technique of protein engineering and indicate how the present state of knowledge allows proteins to be mutated to increase or decrease stability. I discuss experiments on both model proteins and those of relevance to the food industry and show how hydrophobic forces are a major dr...

journal_title：Molecular biotechnology

authors： Goodenough PW

更新日期：1995-10-01 00:00:00

abstract：:The gram-negative anaerobic oral bacterium Porphyromonas gingivalis initiates periodontal disease through fimbrial attachment to saliva-coated oral surfaces. To study the effects of immunomodulation on enhancement of subunit vaccination, the expression in E. coli and immunogenicity of P. gingivalis fimbrial protein (F...

journal_title：Molecular biotechnology

authors： Kim TG,Huy NX,Kim MY,Jeong DK,Jang YS,Yang MS,Langridge WH,Lee JY

更新日期：2009-02-01 00:00:00

abstract：:Enterotoxigenic strains of Escherichia coli produce a heat-labile holotoxin (LT), which causes diarrhea. We engineered corn seeds to produce LT-B, the nontoxic subunit of LT, to serve as a plant-derived vaccine to traveler's diarrhea and as an adjuvant for co-administered proteins. We previously demonstrated that a st...

journal_title：Molecular biotechnology

authors： Karaman S,Cunnick J,Wang K

更新日期：2006-01-01 00:00:00

abstract：:Hypermethylated in Cancer-1 (HIC-1) is a novel tumor suppressor plays crucial role in tumor formation through loss of function by hypermethylation. HIC-1 is known as transcriptional factor whereas little known about its structure and function. Requirement felt to clone and express full coding protein and reveal variou...

journal_title：Molecular biotechnology

authors： Kumar S

更新日期：2014-11-01 00:00:00

abstract：:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...

journal_title：Molecular biotechnology

authors： Kalwy S,Rance J,Young R

更新日期：2006-10-01 00:00:00

abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...

journal_title：Molecular biotechnology

authors： Rotman G,Vanagaite L,Collins FS,Shiloh Y

更新日期：1995-04-01 00:00:00

abstract：:Identification of expressed sequence tags (ESTs) in large genomic segments is an important step in positional cloning and genomic mapping studies. A simple and efficient polymerase chain reaction (PCR)-based approach is described here to identify coding sequences in large genomic fragments of DNA cloned in vectors suc...

journal_title：Molecular biotechnology

authors： Parimoo S

更新日期：1997-12-01 00:00:00

abstract：:Lactic acid bacteria (LAB) are generally sensitive to hydrogen peroxide (H(2)O(2)), Lactobacillus sakei YSI8 is one of the very few LAB strains able to degrade H(2)O(2) through the action of a heme-dependent catalase. Lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product ferment...

journal_title：Molecular biotechnology

authors： An H,Zhou H,Huang Y,Wang G,Luan C,Mou J,Luo Y,Hao Y

更新日期：2010-06-01 00:00:00

abstract：:The concept of photoprotection by dietary means is gaining momentum. Plant constituents such as carotenoids and flavonoids are involved in protection against excess light in plants and contribute to the prevention of UV damage in humans. As micronutrients, they are ingested with the diet and are distributed into light...

journal_title：Molecular biotechnology

authors： Stahl W,Sies H

更新日期：2007-09-01 00:00:00

abstract：:As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...

journal_title：Molecular biotechnology

authors： Hitt DC,Booth JL,Dandapani V,Pennington LR,Gimble JM,Metcalf J

更新日期：2000-03-01 00:00:00

abstract：:The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utilized this property o...

journal_title：Molecular biotechnology

authors： Fuchs B,Zhang K,Rock MG,Bolander ME,Sarkar G

更新日期：1999-10-01 00:00:00

abstract：:To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most pro...

journal_title：Molecular biotechnology

authors： Gach JS,Quendler H,Ferko B,Katinger H,Kunert R

更新日期：2008-06-01 00:00:00

abstract：:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...

journal_title：Molecular biotechnology

authors： Vijayalakshmi MA

更新日期：1996-12-01 00:00:00

abstract：:Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency of gRNA need to be developed. Three gRNAs with ...

journal_title：Molecular biotechnology

authors： Kim DH,Lee J,Suh Y,Lee K

更新日期：2021-02-01 00:00:00

abstract：:Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but ...

journal_title：Molecular biotechnology

authors： Yuan YJ,Ma ZY,Wu JC

更新日期：2002-02-01 00:00:00

abstract：:Association studies using genome scans to identify quantitative trait loci for multifactorial disorders, with anything approaching reasonable power, have been compromised by the need for a very dense array of genetic markers and large numbers of affected individuals. These requirements impose enormous burdens on the g...

journal_title：Molecular biotechnology

authors： Curran S,Hill L,O'Grady G,Turic D,Asherson P,Taylor E,Sham P,Craig I,Vaughan P

更新日期：2002-11-01 00:00:00

abstract：:Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technol...

journal_title：Molecular biotechnology

authors： McCarthy JJ

更新日期：2003-11-01 00:00:00

abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...

journal_title：Molecular biotechnology

authors： Yang Y,Wang Q,Guo D

更新日期：2008-09-01 00:00:00