Abstract:
:The major online single nucleotide polymorphism (SNP) databases freely available as research tools for genetic analysis are explained, reviewed, and compared. An outline is given of the search strategies that can be used with the most extensive current SNP databases: National Centre for Biotechnology Information (NCBI) dbSNP and HapMap to help the user secure the most appropriate data for the research needs of clinical genetics and population genetics research. A range of online tools that can be useful in designing SNP genotyping assays are also detailed.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Phillips Cdoi
10.1385/mb:35:1:65subject
Has Abstractpub_date
2007-01-01 00:00:00pages
65-97issue
1eissn
1073-6085issn
1559-0305pii
MB:35:1:65journal_volume
35pub_type
杂志文章,评审abstract::Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:25:1:95
更新日期:2003-09-01 00:00:00
abstract::Enterotoxigenic strains of Escherichia coli produce a heat-labile holotoxin (LT), which causes diarrhea. We engineered corn seeds to produce LT-B, the nontoxic subunit of LT, to serve as a plant-derived vaccine to traveler's diarrhea and as an adjuvant for co-administered proteins. We previously demonstrated that a st...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:32:1:031
更新日期:2006-01-01 00:00:00
abstract::Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9923-4
更新日期:2016-04-01 00:00:00
abstract::Epoxide hydrolases (EHs; 3.3.2.x) catalyze the enantioselective ring opening of racemic epoxides to the corresponding enantiopure vicinal diols and remaining equivalent unreacted epoxides. These epoxides and diols are used for the synthesis of chiral drug intermediates. With an upsurge in the methods for identificatio...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-017-9996-8
更新日期:2017-03-01 00:00:00
abstract::Derivation of cardiomyocytes directly from patients' own fibroblasts could offer a new therapeutic approach for those with ischemic heart disease. An essential step toward clinical application is to establish safe conversion of human fibroblasts into a cardiac fate. Here we aimed to efficiently and safely generate car...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0007-x
更新日期:2017-06-01 00:00:00
abstract::Brucellosis, one of the most important re-emerging zoonoses in many countries, is caused by bacteria belonging to the genus Brucella. Furthermore these bacteria represent potential biological warfare agents and the identification of species and biovars of field strains may be crucial for tracing back source of infecti...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9659-3
更新日期:2013-10-01 00:00:00
abstract::Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0094-3
更新日期:2018-08-01 00:00:00
abstract::Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated temperatures, including enhanced reaction rates, increased substrate solubility and decreased risks of contamination. Furthermore, thermophilic ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9975-5
更新日期:2016-11-01 00:00:00
abstract::Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase ge...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9094-z
更新日期:2009-01-01 00:00:00
abstract::Transgenic rice seed can be utilized as a bioreactor to produce high-value recombinant proteins. Mouse interleukin 4 (mIL-4) and mIL-6 were specifically expressed as secretory proteins in rice endosperm by ligating the N-terminal glutelin B-1 (GluB-1) signal peptide and the C-terminal KDEL endoplasmic reticulum retent...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9920-7
更新日期:2016-04-01 00:00:00
abstract::In an effort to develop microalgae as a robust system for the production of valuable proteins, we analyzed some of the factors affecting recombinant protein expression in the chloroplast of the green alga Chlamydomonas reinhardtii. We monitored mRNA accumulation, protein synthesis, and protein turnover for three codon...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9348-4
更新日期:2011-05-01 00:00:00
abstract::The diversity and complexity of bioinformatics tools currently available for protein sequence analysis can make it difficult to know where to begin when presented with a new sequence. In this article, we present a protocol outlining one approach to sequence analysis that should give as comprehensive a picture as possi...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-007-0032-2
更新日期:2007-07-01 00:00:00
abstract::Indirubin is a strong inhibitor of several eukaryotic cell signaling pathways and shows promise as a treatment for myelocytic leukemia and Alzheimer's disease. The tmoABCDEF operon, encoding the components of a novel toluene 4-monooxygenase from the paint factory soil isolate, Pseudomonas sp. M4, was cloned and expres...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9863-4
更新日期:2015-08-01 00:00:00
abstract::Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9888-8
更新日期:2015-12-01 00:00:00
abstract::IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalyti...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-007-0003-7
更新日期:2007-06-01 00:00:00
abstract::L-Cysteine desulfhydrase (DES; EC 4.4.1.1) is the most important enzyme that catalyzes the decomposition of L-cysteine to pyruvate, ammonia, and hydrogen sulfide (H2S), the latter of which has recently been recognized as the third gasotransmitter for multiple signaling events in plants. Previous results showed the exi...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9621-9
更新日期:2013-07-01 00:00:00
abstract::Recombinant proteins are useful tools in biological research, drug development, and drug screening. Specially designed expression vectors have been developed to introduce cDNA for recombinant protein expression in mammalian cells. We have combined a dicistronic mRNA design for expression of the recombinant protein, us...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02745860
更新日期:1998-08-01 00:00:00
abstract::Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials us...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:21:1:057
更新日期:2002-05-01 00:00:00
abstract::The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9799-0
更新日期:2015-01-01 00:00:00
abstract::Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technol...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:25:3:275
更新日期:2003-11-01 00:00:00
abstract::The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9479-2
更新日期:2012-10-01 00:00:00
abstract::A functional assay for proteolytic processing of the amyloid precursor protein (APP) was set up in yeast. This consisted of a membrane-bound chimeric protein containing the beta-secretase cleaved C-terminal fragment of APP fused to the Ga14 transcription factor. Using this chimera in a GAL-reporter yeast strain, an ex...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:15:1:29
更新日期:2000-05-01 00:00:00
abstract::Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:14:1:01
更新日期:2000-01-01 00:00:00
abstract::Lentivirus-mediated RNA interference (RNAi) is a potent experimental tool for investigating gene functions in vitro and in vivo. It has advantages that transgenic technology lacks. However, in vivo applications are difficult to apply in the central nervous system of non-model organisms due to the lack of a standard br...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9596-6
更新日期:2013-06-01 00:00:00
abstract::Adeno-associated virus (AAV) is a small, non-enveloped virus used as vector in gene therapy, mainly produced in human cells and in baculovirus systems. Intense studies on these platforms led to the production of vectors with titers between 103 and 105 viral genomes (vg) per cells. In spite of this, vector yields need ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00294-4
更新日期:2021-02-01 00:00:00
abstract::Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to oth...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9384-8
更新日期:2011-10-01 00:00:00
abstract::Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789104
更新日期:1995-04-01 00:00:00
abstract::UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9546-3
更新日期:2013-05-01 00:00:00
abstract::Previous studies have shown that mRNA-electroporated dendritic cells (DCs) are able to process and present tumor-associated antigens, leading to the activation of tumor-specific T cells in vitro and in vivo. However, the optimal maturation state of antigen loading and half-life of the mRNA-translated protein product a...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9071-6
更新日期:2008-10-01 00:00:00
abstract::As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:14:3:197
更新日期:2000-03-01 00:00:00