Abstract:
:Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technology but also on results of empirical studies of the human genome and on genetic epidemiology studies of real drugs in patient populations. This review discusses some of the recent advances in pharmacogenomic research and development over the last few years that include understanding the architecture of the human genome, the creation of population deoxyribonucleic acid (DNA)/data banks, assessment of the clinical validity of genetic markers, and experience with regulatory aspects of pharmacogenomics.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
McCarthy JJdoi
10.1385/MB:25:3:275keywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
275-82issue
3eissn
1073-6085issn
1559-0305pii
MB:25:3:275journal_volume
25pub_type
杂志文章,评审abstract::Murraya koenigii (L.) Spreng. (Rutaceae), is an aromatic plant and much valued for its flavor, nutritive and medicinal properties. In this study, three DNA fingerprinting methods viz., random amplification of polymorphic DNA (RAPD), directed amplification of minisatellite DNA (DAMD), and inter-simple sequence repeat (...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9500-4
更新日期:2013-02-01 00:00:00
abstract::Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ab...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9850-9
更新日期:2015-06-01 00:00:00
abstract::Over the last decade transgenic mouse models have become a common experimental tool for unraveling gene function. During this time there has been a growing expectation that transgenes resemble the in vivo state as much as possible. To this end, a preference away from heterologous promoters has emerged, and transgene c...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:29:2:153
更新日期:2005-02-01 00:00:00
abstract::Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last y...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-011-9402-x
更新日期:2011-11-01 00:00:00
abstract::In this article, four types of standards were assessed in a SYBR Green-based real-time PCR procedure for the quantification of Staphylococcus aureus (S. aureus) in DNA samples. The standards were purified S. aureus genomic DNA (type A), circular plasmid DNA containing a thermonuclease (nuc) gene fragment (type B), DNA...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9417-3
更新日期:2012-01-01 00:00:00
abstract::The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:24:3:229
更新日期:2003-07-01 00:00:00
abstract::Identification of expressed sequence tags (ESTs) in large genomic segments is an important step in positional cloning and genomic mapping studies. A simple and efficient polymerase chain reaction (PCR)-based approach is described here to identify coding sequences in large genomic fragments of DNA cloned in vectors suc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02760779
更新日期:1997-12-01 00:00:00
abstract::Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0021-z
更新日期:2017-08-01 00:00:00
abstract::An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained b...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02752699
更新日期:1998-02-01 00:00:00
abstract::Recent human clinical trials results demonstrated successful treatment for certain genetic forms of cystic fibrosis (CF). To extend treatment opportunities to those afflicted with other genetic forms of CF disease, structural and biophysical characterization of CF transmembrane conductance regulator (CFTR) is urgently...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9830-5
更新日期:2015-05-01 00:00:00
abstract::G-quartets are square planar arrangements of four guanine bases, which can form extraordinarily stable stacks when present in nucleic acid sequences. Such G-quadruplex structures were long regarded as an in vitro phenomenon, but the widespread presence of suitable sequences in genomes and the identification of protein...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-011-9395-5
更新日期:2011-10-01 00:00:00
abstract::An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could repr...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9516-9
更新日期:2013-01-01 00:00:00
abstract::Angiogenesis has been considered an important target for cancer therapy. The inhibition of angiogenesis represents a promising strategy for anti-cancer treatment, tumor growth inhibition, and metastasis. Vasostatin 30 (Vs30), and the 14.1 kDa vasoinhibin (Vi-II-14.1) are two peptides with remarkable anti-tumor and ant...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9642-4
更新日期:2013-07-01 00:00:00
abstract::In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00194-2
更新日期:2019-09-01 00:00:00
abstract::The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02761713
更新日期:1996-12-01 00:00:00
abstract::Brucellosis, one of the most important re-emerging zoonoses in many countries, is caused by bacteria belonging to the genus Brucella. Furthermore these bacteria represent potential biological warfare agents and the identification of species and biovars of field strains may be crucial for tracing back source of infecti...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9659-3
更新日期:2013-10-01 00:00:00
abstract::The implementation of efficient technologies for the production of recombinant mammalian membrane receptors is an outstanding challenge in understanding receptor-ligand actions and the development of therapeutic antibodies. In order to improve the solubility of recombinant extracellular domains of human membrane recep...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:33:3:199
更新日期:2006-07-01 00:00:00
abstract::Papaya ringspot virus (PRSV) causes severe economic losses in both cucurbits and papaya throughout the tropics and subtropics. Development of PRSV-resistant transgenic plants faces a major hurdle in achieving resistance against geographically distinct isolates. One of the major reasons of failing to achieve the broad-...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9205-5
更新日期:2010-01-01 00:00:00
abstract::Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase ge...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9094-z
更新日期:2009-01-01 00:00:00
abstract::This article presents a simple and rapid method for removal of unincorporated label and proteins from DNA sequencing reactions by using Wizard purification resin. This method can be successfully applied for preparation of end-labeled oligonucleotides free of unincorporated label, which is important in experiments (inc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789066
更新日期:1996-04-01 00:00:00
abstract::In this study, we investigated the relative expression of the Rous sarcoma virus (RSV) promoter-driven expression of enhanced green fluorescent protein (EGFP) in fibroblasts of transgenic quails. We analyzed the direct influence of CpG methylation of the RSV promoter on the transcriptional activity of delivered transg...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9393-7
更新日期:2011-11-01 00:00:00
abstract::Epoxide hydrolases (EHs; 3.3.2.x) catalyze the enantioselective ring opening of racemic epoxides to the corresponding enantiopure vicinal diols and remaining equivalent unreacted epoxides. These epoxides and diols are used for the synthesis of chiral drug intermediates. With an upsurge in the methods for identificatio...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-017-9996-8
更新日期:2017-03-01 00:00:00
abstract::The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9479-2
更新日期:2012-10-01 00:00:00
abstract::Pasteurella multocida is the main cause of haemorrhagic septicaemia (HS) outbreak in livestock, such as cattle and buffaloes. Conventional vaccines such as alum-precipitated or oil-adjuvant broth bacterins were injected subcutaneously to provide protection against HS. However, the immunity developed is only for short ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00244-0
更新日期:2020-05-01 00:00:00
abstract::A synthetic gene encoding a single chain Fv fragment of an antibody directed against the nuclear inclusion a (NIa) protein of potato virus Y (PVY) was used to transform two commercial potato cultivars (Claustar and BF15). The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavag...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:33:2:133
更新日期:2006-06-01 00:00:00
abstract::The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia A...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9423-5
更新日期:2012-03-01 00:00:00
abstract::IMGT, the International ImMunoGeneTics information system ( http://imgt.cines.fr ), was created in 1989 by the Laboratoire d'ImmunoGénétique Moléculaire (LIGM) (Université Montpellier 2 and CNRS) at Montpellier, France, in order to standardize and manage the complexity of immunogenetics data. IMGT is recognized as the...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9062-7
更新日期:2008-09-01 00:00:00
abstract::Costimulatory signal(s) in addition to engagement of T cell receptor is important for optimal activation of T lymphocytes. During last decade several dozens of new costimulatory molecules, including 4-1BB, CD40, OX40, CD27, and ICOS, have been identified. It is likely that in Post-Genome Era more molecules with potent...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:21:3:259
更新日期:2002-07-01 00:00:00
abstract::To develop combined vaccine systems, we have generated Edwardsiella tarda ghosts (ETG) displaying a foreign protein on the outer membrane and also Ed. tarda cadaver (ETC) expressing a heterologous protein in the cytoplasm. Green fluorescent protein (GFP) was used as a model foreign protein. A constitutive promoter (Et...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9277-2
更新日期:2010-10-01 00:00:00
abstract::We have previously described a microarray platform combining live basophils with protein arrays suitable for high-throughput detection of sensitisation against allergens. During optimisation of this technique, we observed severe losses of adhering cells during the washing steps, particularly after activation. In order...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9689-x
更新日期:2014-02-01 00:00:00