Abstract:
:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of proteins, with relevant interaction mechanism are described. Practical protocols of sample quality, capacity and scaled up and scaled down operations are discussed. Possibilities of pyrogen removal from high value blood proteins and their simultaneous recovery in the pure form, using histidine immobilized sorbents are described.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Vijayalakshmi MAdoi
10.1007/BF02761713subject
Has Abstractpub_date
1996-12-01 00:00:00pages
347-57issue
3eissn
1073-6085issn
1559-0305journal_volume
6pub_type
杂志文章abstract::An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained b...
journal_title:Molecular biotechnology
pub_type: 杂志文章
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journal_title:Molecular biotechnology
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abstract::ATP-binding cassette transporter G2 (ABCG2) gene encodes a protein that has a wide variety of substrates and is responsible for the active secretion of clinically and toxicologically important molecules into milk. Although known in many species, this marks the first time this gene product has been reported in goats. I...
journal_title:Molecular biotechnology
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doi:10.1007/s12033-013-9653-9
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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更新日期:1995-08-01 00:00:00
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
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更新日期:2012-10-01 00:00:00
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更新日期:2014-02-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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更新日期:1996-06-01 00:00:00
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doi:10.1007/s12033-017-0046-3
更新日期:2018-01-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
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更新日期:2019-06-01 00:00:00
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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更新日期:1999-11-01 00:00:00
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
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doi:10.1007/s12033-018-0080-9
更新日期:2018-05-01 00:00:00
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journal_title:Molecular biotechnology
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更新日期:2002-05-01 00:00:00
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journal_title:Molecular biotechnology
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journal_title:Molecular biotechnology
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9066-3
更新日期:2008-09-01 00:00:00