Isolation of a novel lipase gene from Serratia liquefaciens S33 DB-1, functional expression in Pichia pastoris and its properties.

abstract：:A naturally secreted Gaussia luciferase (Gluc) has been utilized as a reporter for bioluminescence imaging (BLI) evaluation. However, the potential application of Gluc for in vivo monitoring of systemic protein delivery, as well as its natural biodistribution, has not been studied. To examine Gluc secretion and uptake...

journal_title：Molecular biotechnology

authors： El-Amouri SS,Cao P,Miao C,Pan D

更新日期：2013-01-01 00:00:00

abstract：:A short (43-bp) A/T-rich stretch of DNA located in the intergenic region between the baiA2 and baiF genes from Eubacterium sp. strain VPI 12708 was amplified by polymerase chain reaction (PCR) and inserted in front of the Shine-Dalgarno (SD) sequences of three inefficiently-expressed Eubacterium sp. strain VPI 12708 g...

journal_title：Molecular biotechnology

authors： Mallonee DH,Hylemon PB

更新日期：1999-02-01 00:00:00

abstract：:D-Allulose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity. Using D-psicose epimerase and L-rhamnulose kinase, a method enabling high conversio...

journal_title：Molecular biotechnology

authors： Xiao Q,Niu J,Liu H,Liu Y,Zhou X

更新日期：2019-06-01 00:00:00

abstract：:Water-soluble quinoprotein glucose dehydrogenase (PQQGDH-B) is a dimeric enzyme whose application for glucose sensing is the focus of much attention. We attempted to increase the thermal stability of PQQGDH-B by introducing a disulfide bond at the dimer interface. The Ser residue at position 415 was selected for subst...

journal_title：Molecular biotechnology

authors： Igarashi S,Sode K

更新日期：2003-06-01 00:00:00

abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...

journal_title：Molecular biotechnology

authors： Rotman G,Vanagaite L,Collins FS,Shiloh Y

更新日期：1995-04-01 00:00:00

abstract：:Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but ...

journal_title：Molecular biotechnology

authors： Yuan YJ,Ma ZY,Wu JC

更新日期：2002-02-01 00:00:00

abstract：:A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...

journal_title：Molecular biotechnology

authors： Kapros T,Robertson AJ,Waterborg JH

更新日期：1994-08-01 00:00:00

abstract：:Lactic acid bacteria (LAB) are functional starter cultures in food and dairy industry and are also regarded as power houses for bioprocess and fermentation technology. Due to extensive applications in food and medical applications, intensive research and developmental activities are going on throughout the world to un...

journal_title：Molecular biotechnology

authors： Vivek N,Hazeena SH,Rajesh RO,Godan TK,Anjali KB,Nair LM,Mohan B,Nair SC,Sindhu R,Pandey A,Binod P

更新日期：2019-08-01 00:00:00

abstract：:Megaprimer-based methodology has been widely applied in site-directed mutagenesis, but rarely used in gene splicing. In this article, we describe a modification of the megaprimer PCR method, which can efficiently create and amplify a specific ligated chimeric gene segment in a PCR reaction and under a common PCR progr...

journal_title：Molecular biotechnology

authors： Chen JR,Lü JJ,Wang HF

更新日期：2008-11-01 00:00:00

abstract：:To develop combined vaccine systems, we have generated Edwardsiella tarda ghosts (ETG) displaying a foreign protein on the outer membrane and also Ed. tarda cadaver (ETC) expressing a heterologous protein in the cytoplasm. Green fluorescent protein (GFP) was used as a model foreign protein. A constitutive promoter (Et...

journal_title：Molecular biotechnology

authors： Choi SH,Nam YK,Kim KH

更新日期：2010-10-01 00:00:00

abstract：:A total of 41 accessions of Aglianico belonging to three different biotypes (Taburno, Taurasi, and Vulture) and 9 accessions of Sirica grapes were sampled from diverse areas of Campania (Italy). All accessions were first genotyped using 21 microsatellite markers (SSR) to evaluate possible homonymies, synonymies, and t...

journal_title：Molecular biotechnology

authors： De Lorenzis G,Imazio S,Biagini B,Failla O,Scienza A

更新日期：2013-06-01 00:00:00

abstract：:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...

journal_title：Molecular biotechnology

authors： Wang XW,Xiong AS,Yao QH,Zhang Z,Qiao YS

更新日期：2010-01-01 00:00:00

abstract：:The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...

journal_title：Molecular biotechnology

authors： Kulichkova VA,Artamonova TO,Zaykova JJ,Ermolaeva JB,Khodorkovskii MA,Barlev NA,Tomilin AN,Tsimokha AS

更新日期：2015-01-01 00:00:00

abstract：:Molecular biological methods that use antibodies and nucleic acids to detect specific foodborne bacterial pathogens were scarcely known a decade and a half ago. Few scientists could have predicted that these tools of basic research would come to dominate the field of food diagnostics. Today, a large number of cleverly...

journal_title：Molecular biotechnology

authors： Feng P

更新日期：1997-06-01 00:00:00

abstract：:A gene encoding maltogenic amylase from acidic Bacillus sp. US149 (maUS149) was cloned, sequenced and over-expressed in Escherichia coli. The nucleotide sequence analysis revealed an open reading frame (ORF) of 1749 bp encoding a protein of 582 residues. The alignment of deduced amino acid sequence revealed a relative...

journal_title：Molecular biotechnology

authors： Mabrouk SB,Messaoud EB,Ayadi D,Jemli S,Roy A,Mezghani M,Bejar S

更新日期：2008-03-01 00:00:00

abstract：:The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those s...

journal_title：Molecular biotechnology

authors： Chuderland D,Seger R

更新日期：2005-01-01 00:00:00

abstract：:The Embrapa 5.1 genetically modified (GM) common bean was approved for commercialization in Brazil. Methods for the quantification of this new genetically modified organism (GMO) are necessary. The development of a suitable endogenous reference is essential for GMO quantification by real-time PCR. Based on this, a new...

journal_title：Molecular biotechnology

authors： Venturelli GL,Brod FC,Rossi GB,Zimmermann NF,Oliveira JP,Faria JC,Arisi AC

更新日期：2014-11-01 00:00:00

abstract：:Human immunodeficiency virus types 1 and 2 (HIV-1, -2), the etiological agents of AIDS, are retroviruses that replicate in CD4+ T lymphocytes, monocytes, and macrophages. Early anti-HIV therapies were directed at the step in the virus life cycle that was considered the most readily blocked: the transcription of viral ...

journal_title：Molecular biotechnology

authors： Patience C,Moore J,Boyd M

更新日期：1994-02-01 00:00:00

abstract：:Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...

journal_title：Molecular biotechnology

authors： Dabrowski S,Maciuńska J,Synowiecki J

更新日期：1998-12-01 00:00:00

abstract：:Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadv...

journal_title：Molecular biotechnology

authors： Christensen AC

更新日期：2001-03-01 00:00:00

abstract：:In this study cationic β-cyclodextrin-chitosan-mediated nanoparticles were used to transfer pmCherry-C1 into glioblastoma cells and their transfection efficiency were compared to lipofectamine and electroporation. Physicochemical characteristics of nanoparticles were evaluated by photon correlation spectroscopy and sc...

journal_title：Molecular biotechnology

authors： Eslaminejad T,Nematollahi-Mahani SN,Ansari M

更新日期：2016-04-01 00:00:00

abstract：:In this review I briefly describe the technique of protein engineering and indicate how the present state of knowledge allows proteins to be mutated to increase or decrease stability. I discuss experiments on both model proteins and those of relevance to the food industry and show how hydrophobic forces are a major dr...

journal_title：Molecular biotechnology

authors： Goodenough PW

更新日期：1995-10-01 00:00:00

abstract：:Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor, that has been used as a therapeutic agent in facilitating bone marrow and stem cell transplantation and in other clinical cases like neutropenia. Although biologically active recombinant GM-CSF has been successfully produced in...

journal_title：Molecular biotechnology

authors： Bhattacharya P,Pandey G,Srivastava P,Mukherjee KJ

更新日期：2005-06-01 00:00:00

abstract：:Derivation of cardiomyocytes directly from patients' own fibroblasts could offer a new therapeutic approach for those with ischemic heart disease. An essential step toward clinical application is to establish safe conversion of human fibroblasts into a cardiac fate. Here we aimed to efficiently and safely generate car...

journal_title：Molecular biotechnology

authors： Ghazizadeh Z,Rassouli H,Fonoudi H,Alikhani M,Talkhabi M,Darbandi-Azar A,Chen S,Baharvand H,Aghdami N,Salekdeh GH

更新日期：2017-06-01 00:00:00

abstract：:Murraya koenigii (L.) Spreng. (Rutaceae), is an aromatic plant and much valued for its flavor, nutritive and medicinal properties. In this study, three DNA fingerprinting methods viz., random amplification of polymorphic DNA (RAPD), directed amplification of minisatellite DNA (DAMD), and inter-simple sequence repeat (...

journal_title：Molecular biotechnology

authors： Verma S,Rana TS

更新日期：2013-02-01 00:00:00

abstract：:As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...

journal_title：Molecular biotechnology

authors： Hitt DC,Booth JL,Dandapani V,Pennington LR,Gimble JM,Metcalf J

更新日期：2000-03-01 00:00:00

abstract：:Enterotoxigenic strains of Escherichia coli produce a heat-labile holotoxin (LT), which causes diarrhea. We engineered corn seeds to produce LT-B, the nontoxic subunit of LT, to serve as a plant-derived vaccine to traveler's diarrhea and as an adjuvant for co-administered proteins. We previously demonstrated that a st...

journal_title：Molecular biotechnology

authors： Karaman S,Cunnick J,Wang K

更新日期：2006-01-01 00:00:00

abstract：:Virus-like particles (VLPs) have vast potential for applications in nanoscience and nanomedicine. These biological nanoparticles may be used for medical imaging, vaccination, or tissue-specific delivery of drugs or other bioactive molecules. VLPs of Human parvovirus B19 (B19 V) can be assembled in vitro from the recom...

journal_title：Molecular biotechnology

authors： Sánchez-Rodríguez SP,Enrriquez-Avila JV,Soto-Fajardo JM,Peña-Montes C,Bustos-Jaimes I

更新日期：2015-04-01 00:00:00

abstract：:The aim of this study was to evaluate the usefulness of replicating but non disseminating adenovirus vectors (AdVs) as vaccine vector using human rotavirus (HRV) as a model pathogen. HRV VP7, VP4, or VP4Δ (N-terminal 336 amino acids of VP4) structural proteins as well as the VP4Δ::VP7 chimeric fusion protein were expr...

journal_title：Molecular biotechnology

authors： Girard A,Roques E,St-Louis MC,Massie B,Archambault D

更新日期：2013-07-01 00:00:00

abstract：:Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...

journal_title：Molecular biotechnology

authors： Arneaud SL,Porter JR

更新日期：2015-12-01 00:00:00