Abstract:
:In this review I briefly describe the technique of protein engineering and indicate how the present state of knowledge allows proteins to be mutated to increase or decrease stability. I discuss experiments on both model proteins and those of relevance to the food industry and show how hydrophobic forces are a major driving force for folding as well as having a major role in thermostability. I also indicate the large contribution that hydrogen bonding, electrostatic interactions and, in a less well predicted way, disulfide bridges make to thermostability.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Goodenough PWdoi
10.1007/BF02921609subject
Has Abstractpub_date
1995-10-01 00:00:00pages
151-66issue
2eissn
1073-6085issn
1559-0305journal_volume
4pub_type
杂志文章,评审abstract::This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9365-3
更新日期:2011-07-01 00:00:00
abstract::The mapping of genetic loci within organisms has been accelerated by the advent of Radiation Hybrid (RH) panels. These panels are available for humans and non-humans including mice, baboon, rat, and canine. This article contains a general protocol for the use of the Genebridge 4 whole genome RH panel to map a human lo...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:19:2:205
更新日期:2001-10-01 00:00:00
abstract::In mammals, CpG methylation is one of the mechanisms of epigenetic control over the linear sequence of bases of deoxyribonucleic acid (DNA); about 70% of CpG dinucleotides are methylated. The actual signal that triggers DNA methylation is not known, although repetitive DNA has been shown to be an attractive template f...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:26:3:215
更新日期:2004-03-01 00:00:00
abstract::Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789104
更新日期:1995-04-01 00:00:00
abstract::Plants synthesize amino acids by collateral metabolic pathways using primary elements carbon and oxygen from air, hydrogen from water in soil and nitrogen from soil. Following synthesis, amino acids are immediately used for metabolism, transient storage or transported to the phloem. Different families of transporters ...
journal_title:Molecular biotechnology
pub_type: 杂志文章,收录出版
doi:10.1007/s12033-020-00240-4
更新日期:2020-03-01 00:00:00
abstract::Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0140-1
更新日期:2019-02-01 00:00:00
abstract::In this article, four types of standards were assessed in a SYBR Green-based real-time PCR procedure for the quantification of Staphylococcus aureus (S. aureus) in DNA samples. The standards were purified S. aureus genomic DNA (type A), circular plasmid DNA containing a thermonuclease (nuc) gene fragment (type B), DNA...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9417-3
更新日期:2012-01-01 00:00:00
abstract::Murraya koenigii (L.) Spreng. (Rutaceae), is an aromatic plant and much valued for its flavor, nutritive and medicinal properties. In this study, three DNA fingerprinting methods viz., random amplification of polymorphic DNA (RAPD), directed amplification of minisatellite DNA (DAMD), and inter-simple sequence repeat (...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9500-4
更新日期:2013-02-01 00:00:00
abstract::The electron microscopic study of DNA-protein complexes can yield valuable information that is often not easily available by other methods. In this article we give a number of examples that were chosen to illustrate the utility of electron microscopy. Along with the strategy used are protocols that allow such experime...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:16:1:77
更新日期:2000-09-01 00:00:00
abstract::Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:28:1:21
更新日期:2004-09-01 00:00:00
abstract::Megaprimer-based methodology has been widely applied in site-directed mutagenesis, but rarely used in gene splicing. In this article, we describe a modification of the megaprimer PCR method, which can efficiently create and amplify a specific ligated chimeric gene segment in a PCR reaction and under a common PCR progr...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9078-z
更新日期:2008-11-01 00:00:00
abstract::A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vect...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:32:2:093
更新日期:2006-02-01 00:00:00
abstract::Previous studies have shown that mRNA-electroporated dendritic cells (DCs) are able to process and present tumor-associated antigens, leading to the activation of tumor-specific T cells in vitro and in vivo. However, the optimal maturation state of antigen loading and half-life of the mRNA-translated protein product a...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9071-6
更新日期:2008-10-01 00:00:00
abstract::Transgenic rice seed can be utilized as a bioreactor to produce high-value recombinant proteins. Mouse interleukin 4 (mIL-4) and mIL-6 were specifically expressed as secretory proteins in rice endosperm by ligating the N-terminal glutelin B-1 (GluB-1) signal peptide and the C-terminal KDEL endoplasmic reticulum retent...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9920-7
更新日期:2016-04-01 00:00:00
abstract::The major online single nucleotide polymorphism (SNP) databases freely available as research tools for genetic analysis are explained, reviewed, and compared. An outline is given of the search strategies that can be used with the most extensive current SNP databases: National Centre for Biotechnology Information (NCBI...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/mb:35:1:65
更新日期:2007-01-01 00:00:00
abstract::Water-soluble quinoprotein glucose dehydrogenase (PQQGDH-B) is a dimeric enzyme whose application for glucose sensing is the focus of much attention. We attempted to increase the thermal stability of PQQGDH-B by introducing a disulfide bond at the dimer interface. The Ser residue at position 415 was selected for subst...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:24:2:97
更新日期:2003-06-01 00:00:00
abstract::We describe an expression system for high-yield production of recombinant soluble human FasL (rsh- FasL) in CHO cells. After one round of selection for gene amplification, cell lines producing rsh-FasL up to 60 microg/L x 10(6) cells in 24 h were obtained. Cell lines were grown in protein-free medium as suspension cul...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:23:3:189
更新日期:2003-03-01 00:00:00
abstract::Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9066-3
更新日期:2008-09-01 00:00:00
abstract::Efficient display of antibody on filamentous phage M13 coat is crucial for successful biopanning selections. We applied a directed evolution strategy to improve the oligovalent display of a poorly behaving Fab fragment fused to phage gene-3 for minor coat protein (g3p). The Fab displaying clones were enriched from a r...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9231-3
更新日期:2010-03-01 00:00:00
abstract::This review outlines the various methods available for submitting sequence data to the EMBL Nucleotide Sequence Database. Depending on the type of sequence data and the facilities available to the submitter, one method may be more suitable than another. Recent developments have been the World Wide Web submission tool,...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02762322
更新日期:1996-08-01 00:00:00
abstract::Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency of gRNA need to be developed. Three gRNAs with ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00290-8
更新日期:2021-02-01 00:00:00
abstract::The ability to identify disease-associated genes using microarray technology is dependent on isolating high-quality total RNA from the diseased tissue under study. However, it is not always possible to obtain large amounts of affected tissue from patients and therefore the quality and yield of RNA may be compromised. ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:23:2:171
更新日期:2003-02-01 00:00:00
abstract::HOX genes are highly conserved transcription factors that determine the identity of cells and tissues along the anterior-posterior body axis in developing embryos. Aberrations in HOX gene expression have been shown in various tumors. However, the correlation of HOX gene expression patterns with tumorigenesis and cance...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9682-4
更新日期:2014-01-01 00:00:00
abstract::The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes needed for efficient solubilization of native cellulose, the major component of plants. In this work, we reported the molecular characterization of an important glycosyl-hydrolase enzyme classified as endo-β-1,4-glucanase. ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9714-0
更新日期:2014-04-01 00:00:00
abstract::Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9735-3
更新日期:2014-05-01 00:00:00
abstract::IMGT, the International ImMunoGeneTics information system ( http://imgt.cines.fr ), was created in 1989 by the Laboratoire d'ImmunoGénétique Moléculaire (LIGM) (Université Montpellier 2 and CNRS) at Montpellier, France, in order to standardize and manage the complexity of immunogenetics data. IMGT is recognized as the...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9062-7
更新日期:2008-09-01 00:00:00
abstract::Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9923-4
更新日期:2016-04-01 00:00:00
abstract::Virus crystallography can provide atomic resolution structures for intact isometric virus particles and components thereof. The methodology is illustrated by reference to a particularly complex example, the core of the bluetongue virus (700 A). ...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:12:1:13
更新日期:1999-08-01 00:00:00
abstract::Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: int...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9770-0
更新日期:2014-10-01 00:00:00
abstract::The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:24:3:229
更新日期:2003-07-01 00:00:00