Oligovalent Fab display on M13 phage improved by directed evolution.

abstract：:Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...

journal_title：Molecular biotechnology

authors： Madonna R,Delli Pizzi S,Tartaro A,De Caterina R

更新日期：2014-05-01 00:00:00

abstract：:The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia A...

journal_title：Molecular biotechnology

authors： Meneghetti S,Poljuha D,Frare E,Costacurta A,Morreale G,Bavaresco L,Calò A

更新日期：2012-03-01 00:00:00

abstract：:The use of synthetic genes may constitute a successful approach for the heterologous production and functional expression of bacterial antimicrobial peptides (bacteriocins) by recombinant yeasts. In this work, synthetic genes with adapted codon usage designed from the mature amino acid sequence of the bacteriocin ente...

journal_title：Molecular biotechnology

authors： Jiménez JJ,Borrero J,Gútiez L,Arbulu S,Herranz C,Cintas LM,Hernández PE

更新日期：2014-06-01 00:00:00

abstract：:RNA interference methodology suppresses specific gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. Lipofectamine RNAiMAX, a new transfection reagent, has been confirmed high efficiency in delivering small interfering RNA (siRNA) into mesenchymal stem ce...

journal_title：Molecular biotechnology

authors： Zhao M,Yang H,Jiang X,Zhou W,Zhu B,Zeng Y,Yao K,Ren C

更新日期：2008-09-01 00:00:00

abstract：:Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases ...

journal_title：Molecular biotechnology

authors： Dimopoulou M,Claisse O,Dutilh L,Miot-Sertier C,Ballestra P,Lucas PM,Dols-Lafargue M

更新日期：2017-08-01 00:00:00

abstract：:Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated temperatures, including enhanced reaction rates, increased substrate solubility and decreased risks of contamination. Furthermore, thermophilic ...

journal_title：Molecular biotechnology

authors： Mandelli F,Gonçalves TA,Gandin CA,Oliveira AC,Oliveira Neto M,Squina FM

更新日期：2016-11-01 00:00:00

abstract：:This article presents a simple and rapid method for removal of unincorporated label and proteins from DNA sequencing reactions by using Wizard purification resin. This method can be successfully applied for preparation of end-labeled oligonucleotides free of unincorporated label, which is important in experiments (inc...

journal_title：Molecular biotechnology

authors： Kaczorowski T,Sektas M

更新日期：1996-04-01 00:00:00

abstract：:The REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) gene encodes a member of the ERF/AP2 transcription factor family involved in redox homeostasis. The RRTF1 gene shows tissue-specific responsiveness to various abiotic stress treatments including a response to salt stress in roots. An interesting feature of this respo...

journal_title：Molecular biotechnology

authors： Soliman ERS,Meyer P

更新日期：2019-04-01 00:00:00

abstract：:Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...

journal_title：Molecular biotechnology

authors： Pegoraro M,Matić S,Pergolizzi B,Iannarelli L,Rossi AM,Morra M,Noris E

更新日期：2017-10-01 00:00:00

abstract：:We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...

journal_title：Molecular biotechnology

authors： Todorov T,Todorova A,Georgieva B,Mitev V

更新日期：2010-06-01 00:00:00

abstract：:The ability to identify disease-associated genes using microarray technology is dependent on isolating high-quality total RNA from the diseased tissue under study. However, it is not always possible to obtain large amounts of affected tissue from patients and therefore the quality and yield of RNA may be compromised. ...

journal_title：Molecular biotechnology

authors： Murray EJ

更新日期：2003-02-01 00:00:00

abstract：:Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...

journal_title：Molecular biotechnology

authors： Noble JE,Knight AE,Reason AJ,Di Matola A,Bailey MJ

更新日期：2007-10-01 00:00:00

abstract：:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...

journal_title：Molecular biotechnology

authors： Ryu KH,Choi SH,Lee JS

更新日期：2000-01-01 00:00:00

abstract：:Neuroblastoma is the most common extracranial solid tumor of infancy. Amplification of MYCN oncogene is found in approximately 20 % of all neuroblastoma patients and correlates with advanced disease stages, rapid tumor progression, and poor prognosis, making this gene an obvious therapeutic target. However, being a tr...

journal_title：Molecular biotechnology

authors： Sidarovich V,Adami V,Quattrone A

更新日期：2014-07-01 00:00:00

abstract：:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...

journal_title：Molecular biotechnology

authors： Wang XW,Xiong AS,Yao QH,Zhang Z,Qiao YS

更新日期：2010-01-01 00:00:00

abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...

journal_title：Molecular biotechnology

authors： Novo C,Domingos A,Karmali A

更新日期：2001-02-01 00:00:00

abstract：:Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...

journal_title：Molecular biotechnology

authors： Naik V,Kardani J,Roy I

更新日期：2016-04-01 00:00:00

abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...

journal_title：Molecular biotechnology

authors： Yang Y,Wang Q,Guo D

更新日期：2008-09-01 00:00:00

abstract：:The simple use of nonisotopic hybridization probes to detect complementary sequences provides valuable information in a large number of research and commercial applications. In hybridization assays, the four "S's (speed, simplicity, sensitivity, and specificity) are important criteria for determining the choice of pro...

journal_title：Molecular biotechnology

authors： Ruth JL

更新日期：1996-10-01 00:00:00

abstract：:In mammals, CpG methylation is one of the mechanisms of epigenetic control over the linear sequence of bases of deoxyribonucleic acid (DNA); about 70% of CpG dinucleotides are methylated. The actual signal that triggers DNA methylation is not known, although repetitive DNA has been shown to be an attractive template f...

journal_title：Molecular biotechnology

authors： Pena RN,Webster J,Kwan S,Korbel J,Whitelaw BA

更新日期：2004-03-01 00:00:00

abstract：:De novo design provides an in silico toolkit for the design of novel small molecular structures to a set of specified structural constraints. With the avalanche of bioinformatics data, de novo design is ideally suited for exploring molecules that could be useful for chemical genomics. The design process involves manip...

journal_title：Molecular biotechnology

authors： Dean PM

更新日期：2007-11-01 00:00:00

abstract：:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...

journal_title：Molecular biotechnology

authors： Vijayalakshmi MA

更新日期：1996-12-01 00:00:00

abstract：:Lactic acid bacteria (LAB) are generally sensitive to hydrogen peroxide (H(2)O(2)), Lactobacillus sakei YSI8 is one of the very few LAB strains able to degrade H(2)O(2) through the action of a heme-dependent catalase. Lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product ferment...

journal_title：Molecular biotechnology

authors： An H,Zhou H,Huang Y,Wang G,Luan C,Mou J,Luo Y,Hao Y

更新日期：2010-06-01 00:00:00

abstract：:The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...

journal_title：Molecular biotechnology

authors： Long C,Cheng Y,Cui J,Liu J,Gan L,Zeng B,Long M

更新日期：2018-01-01 00:00:00

abstract：:Water-soluble quinoprotein glucose dehydrogenase (PQQGDH-B) is a dimeric enzyme whose application for glucose sensing is the focus of much attention. We attempted to increase the thermal stability of PQQGDH-B by introducing a disulfide bond at the dimer interface. The Ser residue at position 415 was selected for subst...

journal_title：Molecular biotechnology

authors： Igarashi S,Sode K

更新日期：2003-06-01 00:00:00

abstract：:While conducting our research on mutations in the human blood platelet glycoprotein Ib-alpha (GPIbalpha) gene, we detected an unusual deletion of 84 bp. This deletion took place in vitro, during PCR and between two direct repeats. It was observed that the deletion could be detected either by the direct sequencing of t...

journal_title：Molecular biotechnology

authors： Hadj Kacem B,Gargouri J,Gargouri A

更新日期：2008-09-01 00:00:00

abstract：:A new lipase gene designated as SlLipA was isolated from Serratia liquefaciens S33 DB-1 by the genomic-walking method. The cloned gene contained an open reading frame (ORF) of 1,845 bp encoding 615 amino acids with a conserved GXSXG motif. Genome sequence analysis showed that an aldo/keto reductase gene closed to the ...

journal_title：Molecular biotechnology

authors： Yao H,Yu S,Zhang L,Zuo K,Ling H,Zhang F,Tang K

更新日期：2008-02-01 00:00:00

abstract：:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...

journal_title：Molecular biotechnology

authors： Bekli S,Aktas B,Gencer D,Aslim B

更新日期：2019-09-01 00:00:00

abstract：:Angiogenesis has been considered an important target for cancer therapy. The inhibition of angiogenesis represents a promising strategy for anti-cancer treatment, tumor growth inhibition, and metastasis. Vasostatin 30 (Vs30), and the 14.1 kDa vasoinhibin (Vi-II-14.1) are two peptides with remarkable anti-tumor and ant...

journal_title：Molecular biotechnology

authors： Vazquez Rodriguez G,Gonzalez C,De Leon Rodriguez A

更新日期：2013-07-01 00:00:00

abstract：:Derivation of cardiomyocytes directly from patients' own fibroblasts could offer a new therapeutic approach for those with ischemic heart disease. An essential step toward clinical application is to establish safe conversion of human fibroblasts into a cardiac fate. Here we aimed to efficiently and safely generate car...

journal_title：Molecular biotechnology

authors： Ghazizadeh Z,Rassouli H,Fonoudi H,Alikhani M,Talkhabi M,Darbandi-Azar A,Chen S,Baharvand H,Aghdami N,Salekdeh GH

更新日期：2017-06-01 00:00:00