Abstract:
:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in this paper. In our study, it has been shown that the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) has a high binding affinity for the leader sequence of SARS-CoV genome. Effect of temperature on the RNA-DNA hybridization was also examined. This method can provide the affinity of interactions with high sensitivity. Therefore, it will be useful in screening binding candidates for a given RNA target motif with one chip.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Yang Y,Wang Q,Guo Ddoi
10.1007/s12033-008-9066-3subject
Has Abstractpub_date
2008-09-01 00:00:00pages
87-93issue
1eissn
1073-6085issn
1559-0305journal_volume
40pub_type
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