Construction of adenoviral vectors.

abstract：:Megaprimer-based methodology has been widely applied in site-directed mutagenesis, but rarely used in gene splicing. In this article, we describe a modification of the megaprimer PCR method, which can efficiently create and amplify a specific ligated chimeric gene segment in a PCR reaction and under a common PCR progr...

journal_title：Molecular biotechnology

authors： Chen JR,Lü JJ,Wang HF

更新日期：2008-11-01 00:00:00

abstract：:Association studies using genome scans to identify quantitative trait loci for multifactorial disorders, with anything approaching reasonable power, have been compromised by the need for a very dense array of genetic markers and large numbers of affected individuals. These requirements impose enormous burdens on the g...

journal_title：Molecular biotechnology

authors： Curran S,Hill L,O'Grady G,Turic D,Asherson P,Taylor E,Sham P,Craig I,Vaughan P

更新日期：2002-11-01 00:00:00

abstract：:The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of d...

journal_title：Molecular biotechnology

authors： Thanh T,Omar H,Abdullah MP,Chi VT,Noroozi M,Ky H,Napis S

更新日期：2009-10-01 00:00:00

abstract：:D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...

journal_title：Molecular biotechnology

authors： Li P,Li X,Wen Z,Dong R,Li K,Fan H,Wang R,Wang J,Lv M

更新日期：2020-08-01 00:00:00

abstract：:The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia A...

journal_title：Molecular biotechnology

authors： Meneghetti S,Poljuha D,Frare E,Costacurta A,Morreale G,Bavaresco L,Calò A

更新日期：2012-03-01 00:00:00

abstract：:A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...

journal_title：Molecular biotechnology

authors： Wang TT,Cheng WC,Lee BH

更新日期：1998-10-01 00:00:00

abstract：:Gene reporter systems play a key role in gene expression and regulation studies. This review describes the ideal reporter systems, including reporter expression vector design. It summarizes the many uses of genetic reporters and outlines the currently available and commonly used reporter systems. Each system is descri...

journal_title：Molecular biotechnology

authors： Schenborn E,Groskreutz D

更新日期：1999-11-01 00:00:00

abstract：:The ability to identify disease-associated genes using microarray technology is dependent on isolating high-quality total RNA from the diseased tissue under study. However, it is not always possible to obtain large amounts of affected tissue from patients and therefore the quality and yield of RNA may be compromised. ...

journal_title：Molecular biotechnology

authors： Murray EJ

更新日期：2003-02-01 00:00:00

abstract：:Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches h...

journal_title：Molecular biotechnology

authors： Morokuma D,Xu J,Hino M,Mon H,Merzaban JS,Takahashi M,Kusakabe T,Lee JM

更新日期：2017-05-01 00:00:00

abstract：:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...

journal_title：Molecular biotechnology

authors： Ryu KH,Choi SH,Lee JS

更新日期：2000-01-01 00:00:00

abstract：:NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expans...

journal_title：Molecular biotechnology

authors： Pedroso JF,de Souza Valim V,Pezzi A,Furlan JM,Lenhart G,Sehn F,Zambonato B,Gonçalves AD,Wilke I,Amorin B,da Silva MA,Pedrazzani FS,da Rocha Silla LM

更新日期：2020-05-01 00:00:00

abstract：:Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadv...

journal_title：Molecular biotechnology

authors： Christensen AC

更新日期：2001-03-01 00:00:00

abstract：:Clinical molecular genetics has recently become recognized as a diagnostic discipline. This article covers the evolution, structure, and possible forward development of clinical molecular genetics. Topics covered include general test categories, introducing new tests, laboratory facilities, staffing and training, and ...

journal_title：Molecular biotechnology

authors： Elles R

更新日期：1997-10-01 00:00:00

abstract：:A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vect...

journal_title：Molecular biotechnology

authors： Kang TJ,Kim BG,Yang JY,Yang MS

更新日期：2006-02-01 00:00:00

abstract：:The diversity and complexity of bioinformatics tools currently available for protein sequence analysis can make it difficult to know where to begin when presented with a new sequence. In this article, we present a protocol outlining one approach to sequence analysis that should give as comprehensive a picture as possi...

journal_title：Molecular biotechnology

authors： Russell RB

更新日期：2007-07-01 00:00:00

abstract：:The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those s...

journal_title：Molecular biotechnology

authors： Chuderland D,Seger R

更新日期：2005-01-01 00:00:00

abstract：:Genetically engineered animal models have been and will continue to be invaluable for exploring the basic mechanisms involved in the aging process as well as in extending our understanding of diseases found to be more prevalent in the older human population. Continued development of such in vivo systems will allow sci...

journal_title：Molecular biotechnology

authors： Andersen JK

更新日期：2001-09-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...

journal_title：Molecular biotechnology

authors： Hristodorov D,Fischer R,Joerissen H,Müller-Tiemann B,Apeler H,Linden L

更新日期：2013-03-01 00:00:00

abstract：:Cyclooxygenase (COX)-2 plays an important role in the development of cancer and has been recognized as a potential therapeutic target. Because nonsteroidal anti-inflammatory drugs (NSAIDs) are able to inhibit the activity of this enzyme, the potential efficacy of such drugs for purposes of cancer prevention or therapy...

journal_title：Molecular biotechnology

authors： Liu YT,Kardosh A,Cooc J,Schönthal AH

更新日期：2006-11-01 00:00:00

abstract：:This review outlines the various methods available for submitting sequence data to the EMBL Nucleotide Sequence Database. Depending on the type of sequence data and the facilities available to the submitter, one method may be more suitable than another. Recent developments have been the World Wide Web submission tool,...

journal_title：Molecular biotechnology

authors： Tuli MA,Flores TP,Cameron GN

更新日期：1996-08-01 00:00:00

abstract：:Terminal Restriction Fragment Length Polymorphism (T-RFLP) or Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (FluRFLP) have made a significant impact on the way in which PCR products amplified from mixed community DNA extracts have been assessed. Technically, these approaches are essent...

journal_title：Molecular biotechnology

authors： Bruce KD,Hughes MR

更新日期：2000-11-01 00:00:00

abstract：:The Delta12 desaturase represents a diverse gene family in plants and is responsible for conversion of oleic acid (18:1) to linoleic acid (18:2). Several members of this family are known from plants like Arabidopsis and Soybean. Using primers from conserved C- and N-terminal regions, we have cloned a novel Delta12 des...

journal_title：Molecular biotechnology

authors： Khadake RM,Ranjekar PK,Harsulkar AM

更新日期：2009-06-01 00:00:00

abstract：:Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but ...

journal_title：Molecular biotechnology

authors： Yuan YJ,Ma ZY,Wu JC

更新日期：2002-02-01 00:00:00

abstract：:This article reviews some of the issues involved in evaluating vaccines in humans. Vaccine trials are required for licensure and are essential for demonstrating a vaccine's safety and protective efficacy. The formal framework of phase I, II, and III trials is described, with particular emphasis on the choice of hypoth...

journal_title：Molecular biotechnology

authors： Farrington CP,Miller E

更新日期：2001-01-01 00:00:00

abstract：:Lactococcus lactis is industrially important microorganism used in many dairy fermentations. Numerous genes and gene expression signals from this organism have now been identified and characterized. Recently, several naturally occurring, inducible gene-expression systems have also been described in L. lactis. The main...

journal_title：Molecular biotechnology

authors： Djordjevic GM,Klaenhammer TR

更新日期：1998-04-01 00:00:00

abstract：:The major online single nucleotide polymorphism (SNP) databases freely available as research tools for genetic analysis are explained, reviewed, and compared. An outline is given of the search strategies that can be used with the most extensive current SNP databases: National Centre for Biotechnology Information (NCBI...

journal_title：Molecular biotechnology

authors： Phillips C

更新日期：2007-01-01 00:00:00

abstract：:A sandwich-type enzyme-linked immunosorbent assay (ELISA) was established for monitoring the secretion of ZZ-fusion proteins. Two antibodies, a monoclonal mouse anti-human proinsulin and a rabbit anti-bovine IgG (strongly binding to the ZZ-domain), were used to quantify the secretion of recombinant human ZZ-proinsulin...

journal_title：Molecular biotechnology

authors： Mergulhão FJ,Monteiro GA,Cabral JM,Taipa MA

更新日期：2001-11-01 00:00:00

abstract：:Alternaria blight caused by Alternaria brassicicola (Schwein.) Wiltshire and A. brassicae (Berk.) Sacc., is one of the most important disease of rapeseed-mustard, characterized by the formation of spots on leaves, stem, and siliquae with premature defoliation and stunting of growth. These two species are very difficul...

journal_title：Molecular biotechnology

authors： Singh R,Kumar S,Kashyap PL,Srivastava AK,Mishra S,Sharma AK

更新日期：2014-11-01 00:00:00

abstract：:Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase ge...

journal_title：Molecular biotechnology

authors： Banerjee A,Dubey S,Kaul P,Barse B,Piotrowski M,Banerjee UC

更新日期：2009-01-01 00:00:00

abstract：:Recent human clinical trials results demonstrated successful treatment for certain genetic forms of cystic fibrosis (CF). To extend treatment opportunities to those afflicted with other genetic forms of CF disease, structural and biophysical characterization of CF transmembrane conductance regulator (CFTR) is urgently...

journal_title：Molecular biotechnology

authors： Hildebrandt E,Mulky A,Ding H,Dai Q,Aleksandrov AA,Bajrami B,Diego PA,Wu X,Ray M,Naren AP,Riordan JR,Yao X,DeLucas LJ,Urbatsch IL,Kappes JC

更新日期：2015-05-01 00:00:00