Abstract:
:Genetically engineered animal models have been and will continue to be invaluable for exploring the basic mechanisms involved in the aging process as well as in extending our understanding of diseases found to be more prevalent in the older human population. Continued development of such in vivo systems will allow scientists to further dissect the role genetic and environmental factors play in aging and in age-related disease states and to enhance our understanding of these processes. In this article we discuss techniques involved in the development of such models and review some examples of laboratory mouse strains that have been used to study either normal aging or select diseases associated with aging.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Andersen JKdoi
10.1385/MB:19:1:045keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
45-57issue
1eissn
1073-6085issn
1559-0305pii
MB:19:1:045journal_volume
19pub_type
杂志文章,评审abstract::The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0080-9
更新日期:2018-05-01 00:00:00
abstract::A synthetic gene encoding a single chain Fv fragment of an antibody directed against the nuclear inclusion a (NIa) protein of potato virus Y (PVY) was used to transform two commercial potato cultivars (Claustar and BF15). The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavag...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:33:2:133
更新日期:2006-06-01 00:00:00
abstract::The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia A...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9423-5
更新日期:2012-03-01 00:00:00
abstract::As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:14:3:197
更新日期:2000-03-01 00:00:00
abstract::A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02760858
更新日期:1998-10-01 00:00:00
abstract::Plants synthesize amino acids by collateral metabolic pathways using primary elements carbon and oxygen from air, hydrogen from water in soil and nitrogen from soil. Following synthesis, amino acids are immediately used for metabolism, transient storage or transported to the phloem. Different families of transporters ...
journal_title:Molecular biotechnology
pub_type: 杂志文章,收录出版
doi:10.1007/s12033-020-00240-4
更新日期:2020-03-01 00:00:00
abstract::Several mutations were introduced into the Cry1Ac toxin gene, resulting in four variants with altered sequences that were responsible for low expression of the toxin in transgenic plants. These variants were as follows: V1, with modified three A/T-rich regions, including the first signal of transcription termination; ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/mb:26:1:17
更新日期:2004-01-01 00:00:00
abstract::Human adipose tissue-derived stromal cells (ADSCs) are being evaluated for cardiovascular repair. We developed an ex vivo method for producing angiogenic ADSCs transduced with a self-inactivating lentiviral vector (LV) expressing the enhanced green fluorescence protein (EGFP) from an internal cytomegalovirus (CMV) pro...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9537-4
更新日期:2013-05-01 00:00:00
abstract::Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9888-8
更新日期:2015-12-01 00:00:00
abstract::Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0065-8
更新日期:2018-03-01 00:00:00
abstract::In this study, human alpha-1,4-N-acetylglucosaminyltransferase (alpha4GnT) fused with GFP(uv) (GFP(uv)-alpha4GnT) was expressed using both a transformed cell system and silkworm larvae. A Tn-pXgp-GFP(uv)-alpha4GnT cell line, isolated after expression vector transfection, produced 106 mU/ml of alpha4GnT activity in sus...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9174-8
更新日期:2009-09-01 00:00:00
abstract::Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic analyses of the gut of C. curvignathus revealed...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0015-x
更新日期:2017-07-01 00:00:00
abstract::The gram-negative anaerobic oral bacterium Porphyromonas gingivalis initiates periodontal disease through fimbrial attachment to saliva-coated oral surfaces. To study the effects of immunomodulation on enhancement of subunit vaccination, the expression in E. coli and immunogenicity of P. gingivalis fimbrial protein (F...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9102-3
更新日期:2009-02-01 00:00:00
abstract::Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ab...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9850-9
更新日期:2015-06-01 00:00:00
abstract::The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0046-3
更新日期:2018-01-01 00:00:00
abstract::Lactic acid bacteria (LAB) are functional starter cultures in food and dairy industry and are also regarded as power houses for bioprocess and fermentation technology. Due to extensive applications in food and medical applications, intensive research and developmental activities are going on throughout the world to un...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-019-00186-2
更新日期:2019-08-01 00:00:00
abstract::Hypermethylated in Cancer-1 (HIC-1) is a novel tumor suppressor plays crucial role in tumor formation through loss of function by hypermethylation. HIC-1 is known as transcriptional factor whereas little known about its structure and function. Requirement felt to clone and express full coding protein and reveal variou...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9783-8
更新日期:2014-11-01 00:00:00
abstract::In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9429-z
更新日期:2012-02-01 00:00:00
abstract::The polyamines spermine, spermidine, and putrescine are small organic molecules one or more of which are present in all living organisms. Many natural products contain polyamine residues. Polyamines are synthesized by a highly regulated pathway from arginine or ornithine and also can be transported in and out of cells...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02788682
更新日期:1999-06-01 00:00:00
abstract::CD151 is an abundantly expressed eukaryotic transmembrane protein on the cell surface. It is involved in cell adhesion, angiogenesis and signal transduction as well in disease conditions such as cancer and viral infections. However, the molecular mechanism of CD151 activation is poorly understood due to the lack of st...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00212-3
更新日期:2019-12-01 00:00:00
abstract::A gene encoding maltogenic amylase from acidic Bacillus sp. US149 (maUS149) was cloned, sequenced and over-expressed in Escherichia coli. The nucleotide sequence analysis revealed an open reading frame (ORF) of 1749 bp encoding a protein of 582 residues. The alignment of deduced amino acid sequence revealed a relative...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-007-9017-4
更新日期:2008-03-01 00:00:00
abstract::This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be den...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02779017
更新日期:1995-12-01 00:00:00
abstract::Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated temperatures, including enhanced reaction rates, increased substrate solubility and decreased risks of contamination. Furthermore, thermophilic ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9975-5
更新日期:2016-11-01 00:00:00
abstract::Lactococcus lactis is industrially important microorganism used in many dairy fermentations. Numerous genes and gene expression signals from this organism have now been identified and characterized. Recently, several naturally occurring, inducible gene-expression systems have also been described in L. lactis. The main...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02760814
更新日期:1998-04-01 00:00:00
abstract::Many preclinical studies have shown RNA interference (RNAi) as a new promising way to treat various human diseases including cancer and virus infection and there is an increasing demand for the large-scale preparation of short interfering RNAs (siRNAs) at low cost. Data are accumulating to show that endoribonuclease-p...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:31:3:203
更新日期:2005-11-01 00:00:00
abstract::NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expans...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00250-2
更新日期:2020-05-01 00:00:00
abstract::An endochitinase gene from the Serratia marcescens Nima strain (chiA Nima) was cloned, sequenced, and expressed in Escherichia coli DH5alphaF', and the recombinant protein (ChiA Nima) was purified by hydrophobic interaction chromatography. chiA Nima contains an open reading frame (ORF) that encodes an endochitinase wi...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:31:2:103
更新日期:2005-10-01 00:00:00
abstract::The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02900362
更新日期:1996-06-01 00:00:00
abstract::A shuttle vector pZL1 which can replicate both in Gluconobacter oxydans and Escherichia coli was constructed based on G. oxydans DSM2003 cryptic plasmid pGOX3, a homology of G. oxydans 621H pGOX3, and E. coli cloning vector pUC18. It was found to be stably maintained in G. oxydans during the serial subcultures in the ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-010-9293-2
更新日期:2010-11-01 00:00:00
abstract::Molecular biological methods that use antibodies and nucleic acids to detect specific foodborne bacterial pathogens were scarcely known a decade and a half ago. Few scientists could have predicted that these tools of basic research would come to dominate the field of food diagnostics. Today, a large number of cleverly...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02740817
更新日期:1997-06-01 00:00:00