Long-term engraftment and angiogenic properties of lentivirally transduced adipose tissue-derived stromal cells.

abstract：:Biobanks, more formally known as biological resource centers (BRCs), form an "unsung" yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and cell-lines would be effectively impossible. BRCs...

journal_title：Molecular biotechnology

authors： Day JG,Stacey GN

更新日期：2008-10-01 00:00:00

abstract：:Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...

journal_title：Molecular biotechnology

authors： Samuel RVM,Farrukh SY,Rehmat S,Hanif MU,Ahmed SS,Musharraf SG,Durrani FG,Saleem M,Gul R

更新日期：2018-08-01 00:00:00

abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...

journal_title：Molecular biotechnology

authors： Yang Y,Wang Q,Guo D

更新日期：2008-09-01 00:00:00

abstract：:The recombinant acidstable α-amylase (Ba-amy) of acidophilic bacterium Bacillus acidicola TSAS1 has been produced extracellularly using a combination of cloning (E. coli and P. pastoris) and physico-chemical treatment strategies. A total of 150,000 U/L of Ba-amy were attained under constitutive promoter in P. pastoris...

journal_title：Molecular biotechnology

authors： Parashar D,Satyanarayana T

更新日期：2016-11-01 00:00:00

abstract：:In this article, four types of standards were assessed in a SYBR Green-based real-time PCR procedure for the quantification of Staphylococcus aureus (S. aureus) in DNA samples. The standards were purified S. aureus genomic DNA (type A), circular plasmid DNA containing a thermonuclease (nuc) gene fragment (type B), DNA...

journal_title：Molecular biotechnology

authors： Martinon A,Cronin UP,Wilkinson MG

更新日期：2012-01-01 00:00:00

abstract：:The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...

journal_title：Molecular biotechnology

authors： Isik N,White L,Barnes R,Poynton CJ,Mills KI

更新日期：2003-07-01 00:00:00

abstract：:The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...

journal_title：Molecular biotechnology

authors： Devon RS,Brookes AJ

更新日期：1996-06-01 00:00:00

abstract：:The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...

journal_title：Molecular biotechnology

authors： Kulichkova VA,Artamonova TO,Zaykova JJ,Ermolaeva JB,Khodorkovskii MA,Barlev NA,Tomilin AN,Tsimokha AS

更新日期：2015-01-01 00:00:00

abstract：:Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utiliz...

journal_title：Molecular biotechnology

authors： Wang X,Tian W,Li Y

更新日期：2008-01-01 00:00:00

abstract：:Terminal Restriction Fragment Length Polymorphism (T-RFLP) or Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (FluRFLP) have made a significant impact on the way in which PCR products amplified from mixed community DNA extracts have been assessed. Technically, these approaches are essent...

journal_title：Molecular biotechnology

authors： Bruce KD,Hughes MR

更新日期：2000-11-01 00:00:00

abstract：:Human papillomavirus (HPV) is the causal agent of cervical cancer, one of the most common causes of death in women worldwide, and its E7 antigen is the major candidate for a therapeutic vaccine. The large scale production of E7 by molecular farming that would lead to the development of a safe and inexpensive vaccine i...

journal_title：Molecular biotechnology

authors： Morgenfeld M,Lentz E,Segretin ME,Alfano EF,Bravo-Almonacid F

更新日期：2014-11-01 00:00:00

abstract：:Neuroblastoma is the most common extracranial solid tumor of infancy. Amplification of MYCN oncogene is found in approximately 20 % of all neuroblastoma patients and correlates with advanced disease stages, rapid tumor progression, and poor prognosis, making this gene an obvious therapeutic target. However, being a tr...

journal_title：Molecular biotechnology

authors： Sidarovich V,Adami V,Quattrone A

更新日期：2014-07-01 00:00:00

abstract：:Cyclooxygenase (COX)-2 plays an important role in the development of cancer and has been recognized as a potential therapeutic target. Because nonsteroidal anti-inflammatory drugs (NSAIDs) are able to inhibit the activity of this enzyme, the potential efficacy of such drugs for purposes of cancer prevention or therapy...

journal_title：Molecular biotechnology

authors： Liu YT,Kardosh A,Cooc J,Schönthal AH

更新日期：2006-11-01 00:00:00

abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...

journal_title：Molecular biotechnology

authors： Novo C,Domingos A,Karmali A

更新日期：2001-02-01 00:00:00

abstract：:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...

journal_title：Molecular biotechnology

authors： Kalwy S,Rance J,Young R

更新日期：2006-10-01 00:00:00

abstract：:An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained b...

journal_title：Molecular biotechnology

authors： Weber K,Bolander ME,Sarkar G

更新日期：1998-02-01 00:00:00

abstract：:Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor, that has been used as a therapeutic agent in facilitating bone marrow and stem cell transplantation and in other clinical cases like neutropenia. Although biologically active recombinant GM-CSF has been successfully produced in...

journal_title：Molecular biotechnology

authors： Bhattacharya P,Pandey G,Srivastava P,Mukherjee KJ

更新日期：2005-06-01 00:00:00

abstract：:There is a growing pharmaceutical market for protein-based drugs for use in therapy and diagnosis. The rapid developments in molecular and cell biology have resulted in production of expression systems for manufacturing of recombinant proteins and monoclonal antibodies. These proteins are glycosylated when expressed i...

journal_title：Molecular biotechnology

authors： Nilsson B

更新日期：1994-12-01 00:00:00

abstract：:Recombinant proteins are useful tools in biological research, drug development, and drug screening. Specially designed expression vectors have been developed to introduce cDNA for recombinant protein expression in mammalian cells. We have combined a dicistronic mRNA design for expression of the recombinant protein, us...

journal_title：Molecular biotechnology

authors： Pu H,Cashion LM,Kretschmer PJ,Liu Z

更新日期：1998-08-01 00:00:00

abstract：:Host protein synthesis is shut down in the lytic baculovirus expression vector system (BEVS). This also affects host proteins involved in routing secretory proteins through the endoplasmic reticulum (ER)-Golgi system. It has been demonstrated that a secretory alkaline phosphatase-EGFP fusion protein (SEFP) can act as ...

journal_title：Molecular biotechnology

authors： Teng CY,Chang SL,van Oers MM,Wu TY

更新日期：2013-05-01 00:00:00

abstract：:Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study, a thermostable pectin methylesterase (CtPME) from Clostridium thermocellum belonging to family 8 carbohydrate...

journal_title：Molecular biotechnology

authors： Rajulapati V,Goyal A

更新日期：2017-05-01 00:00:00

abstract：:G-quartets are square planar arrangements of four guanine bases, which can form extraordinarily stable stacks when present in nucleic acid sequences. Such G-quadruplex structures were long regarded as an in vitro phenomenon, but the widespread presence of suitable sequences in genomes and the identification of protein...

journal_title：Molecular biotechnology

authors： Bryan TM,Baumann P

更新日期：2011-10-01 00:00:00

abstract：:Several mutations were introduced into the Cry1Ac toxin gene, resulting in four variants with altered sequences that were responsible for low expression of the toxin in transgenic plants. These variants were as follows: V1, with modified three A/T-rich regions, including the first signal of transcription termination; ...

journal_title：Molecular biotechnology

authors： Misztal LH,Mostowska A,Skibinska M,Bajsa J,Musial WG,Jarmolowski A

更新日期：2004-01-01 00:00:00

abstract：:Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...

journal_title：Molecular biotechnology

authors： Naik V,Kardani J,Roy I

更新日期：2016-04-01 00:00:00

abstract：:This article describes the construction, expression, and purification of RNase single-chain antibody fusion proteins. To construct a fusion protein, the gene for each moiety, the RNase and the binding ligand, is modified separately to contain complementary DNA encoding a 13 amino acid spacer that separates the RNase f...

journal_title：Molecular biotechnology

authors： Newton DL,Rybak SM

更新日期：2002-01-01 00:00:00

abstract：:The concept of photoprotection by dietary means is gaining momentum. Plant constituents such as carotenoids and flavonoids are involved in protection against excess light in plants and contribute to the prevention of UV damage in humans. As micronutrients, they are ingested with the diet and are distributed into light...

journal_title：Molecular biotechnology

authors： Stahl W,Sies H

更新日期：2007-09-01 00:00:00

abstract：:Murraya koenigii (L.) Spreng. (Rutaceae), is an aromatic plant and much valued for its flavor, nutritive and medicinal properties. In this study, three DNA fingerprinting methods viz., random amplification of polymorphic DNA (RAPD), directed amplification of minisatellite DNA (DAMD), and inter-simple sequence repeat (...

journal_title：Molecular biotechnology

authors： Verma S,Rana TS

更新日期：2013-02-01 00:00:00

abstract：:IMGT, the International ImMunoGeneTics information system ( http://imgt.cines.fr ), was created in 1989 by the Laboratoire d'ImmunoGénétique Moléculaire (LIGM) (Université Montpellier 2 and CNRS) at Montpellier, France, in order to standardize and manage the complexity of immunogenetics data. IMGT is recognized as the...

journal_title：Molecular biotechnology

authors： Lefranc MP

更新日期：2008-09-01 00:00:00

abstract：:RNA interference methodology suppresses specific gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. Lipofectamine RNAiMAX, a new transfection reagent, has been confirmed high efficiency in delivering small interfering RNA (siRNA) into mesenchymal stem ce...

journal_title：Molecular biotechnology

authors： Zhao M,Yang H,Jiang X,Zhou W,Zhu B,Zeng Y,Yao K,Ren C

更新日期：2008-09-01 00:00:00

abstract：:An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could repr...

journal_title：Molecular biotechnology

authors： Wang Z,Wang HY,Feng H

更新日期：2013-01-01 00:00:00