Abstract：
:Cyclooxygenase (COX)-2 plays an important role in the development of cancer and has been recognized as a potential therapeutic target. Because nonsteroidal anti-inflammatory drugs (NSAIDs) are able to inhibit the activity of this enzyme, the potential efficacy of such drugs for purposes of cancer prevention or therapy is an area of intense research. Therefore, it is of critical importance to unequivocally determine the expression levels of COX-2 protein in tumor cells. In this regard, there are several conflicting reports in the literature where the same type of tumor cell lines were reported as COX-2 positive and as COX-2 negative. We found that during Western blot analysis of COX-2 positive and COX-2 negative cells, different antibodies to COX-2 protein are able to generate strong signals, which are false-positives and can be confused with COX-2. Thus, we believe that some of the conflicting reports on COX-2 expression in tumor cell lines could be the result of improper interpretation of the Western blot signals. Here, we present some of these pitfalls and suggest the inclusion of appropriate controls to unequivocally identify COX-2 protein levels.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Liu YT,Kardosh A,Cooc J,Schönthal AHdoi
10.1385/MB:34:3:329subject
Has Abstractpub_date
2006-11-01 00:00:00pages
329-35issue
3eissn
1073-6085issn
1559-0305pii
MB:34:3:329journal_volume
34pub_type
杂志文章abstract：:Electroporation of cells in the presence of DNA is widely used for the introduction of transgenes either stably or transiently into bacterial, fungal, animal, and plant cells. A review of the literature shows that electroporation parameters are often reported in an incomplete or incorrect manner, forcing researchers t...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02821542
更新日期：1997-02-01 00:00:00
abstract：:We report a rapid and simple method for isolating the 5'-end of plant genes from genomic DNA by polymerase chain reaction (PCR) with TATA-box-based degenerate primers (TDPs). The TDPs were specially designed according to the TATA box, which is conserved in the promoter region of most plant genes. The unknown 5'-ends o...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9323-0
更新日期：2011-02-01 00:00:00
abstract：:Immobilized-metal-ion affinity chromatography (IMAC) is used extensively for phosphopeptide enrichment in phosphoproteomics. However, the effect of nucleic acids in protein samples on phosphopeptide enrichment by IMAC has not yet been well clarified. In this study, we demonstrate that IMAC beads possess a strong adsor...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9176-6
更新日期：2009-09-01 00:00:00
abstract：:Virus crystallography can provide atomic resolution structures for intact isometric virus particles and components thereof. The methodology is illustrated by reference to a particularly complex example, the core of the bluetongue virus (700 A). ...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:12:1:13
更新日期：1999-08-01 00:00:00
abstract：:Virus-like particles (VLPs) have vast potential for applications in nanoscience and nanomedicine. These biological nanoparticles may be used for medical imaging, vaccination, or tissue-specific delivery of drugs or other bioactive molecules. VLPs of Human parvovirus B19 (B19 V) can be assembled in vitro from the recom...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9823-4
更新日期：2015-04-01 00:00:00
abstract：:Human papillomavirus (HPV) is the causal agent of cervical cancer, one of the most common causes of death in women worldwide, and its E7 antigen is the major candidate for a therapeutic vaccine. The large scale production of E7 by molecular farming that would lead to the development of a safe and inexpensive vaccine i...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9781-x
更新日期：2014-11-01 00:00:00
abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789104
更新日期：1995-04-01 00:00:00
abstract：:DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:20:3:315
更新日期：2002-03-01 00:00:00
abstract：:This article reviews some of the issues involved in evaluating vaccines in humans. Vaccine trials are required for licensure and are essential for demonstrating a vaccine's safety and protective efficacy. The formal framework of phase I, II, and III trials is described, with particular emphasis on the choice of hypoth...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:17:1:43
更新日期：2001-01-01 00:00:00
abstract：:DNA damage is one of the most impactful events in living organisms, leading to DNA sequence changes (mutation) and disruption of biological processes. A study has identified a protein called Damage Suppressor Protein (Dsup) in the tardigrade Ramazzotius varieornatus that has shown to reduce the effects of radiation da...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00273-9
更新日期：2020-12-01 00:00:00
abstract：:D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00257-9
更新日期：2020-08-01 00:00:00
abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9066-3
更新日期：2008-09-01 00:00:00
abstract：:The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes needed for efficient solubilization of native cellulose, the major component of plants. In this work, we reported the molecular characterization of an important glycosyl-hydrolase enzyme classified as endo-β-1,4-glucanase. ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9714-0
更新日期：2014-04-01 00:00:00
abstract：:A gene encoding maltogenic amylase from acidic Bacillus sp. US149 (maUS149) was cloned, sequenced and over-expressed in Escherichia coli. The nucleotide sequence analysis revealed an open reading frame (ORF) of 1749 bp encoding a protein of 582 residues. The alignment of deduced amino acid sequence revealed a relative...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-9017-4
更新日期：2008-03-01 00:00:00
abstract：:Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9531-x
更新日期：2013-03-01 00:00:00
abstract：:HOX genes are highly conserved transcription factors that determine the identity of cells and tissues along the anterior-posterior body axis in developing embryos. Aberrations in HOX gene expression have been shown in various tumors. However, the correlation of HOX gene expression patterns with tumorigenesis and cance...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9682-4
更新日期：2014-01-01 00:00:00
abstract：:Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated temperatures, including enhanced reaction rates, increased substrate solubility and decreased risks of contamination. Furthermore, thermophilic ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9975-5
更新日期：2016-11-01 00:00:00
abstract：:The electron microscopic study of DNA-protein complexes can yield valuable information that is often not easily available by other methods. In this article we give a number of examples that were chosen to illustrate the utility of electron microscopy. Along with the strategy used are protocols that allow such experime...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:16:1:77
更新日期：2000-09-01 00:00:00
abstract：:Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0021-z
更新日期：2017-08-01 00:00:00
abstract：:The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0080-9
更新日期：2018-05-01 00:00:00
abstract：:Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00281-9
更新日期：2021-01-01 00:00:00
abstract：:High-quality RNA preparations are critical for further applications such as reverse transcriptase-polymerase chain reaction (RT-PCR) transcript amplifications, and elaboration of cDNA and expressed sequence tag libraries. Melanins are phenolic compounds present in many fungi and apparently play key roles in fungi path...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:34:1:45
更新日期：2006-09-01 00:00:00
abstract：:Gene reporter systems play a key role in gene expression and regulation studies. This review describes the ideal reporter systems, including reporter expression vector design. It summarizes the many uses of genetic reporters and outlines the currently available and commonly used reporter systems. Each system is descri...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:13:1:29
更新日期：1999-11-01 00:00:00
abstract：:A functional assay for proteolytic processing of the amyloid precursor protein (APP) was set up in yeast. This consisted of a membrane-bound chimeric protein containing the beta-secretase cleaved C-terminal fragment of APP fused to the Ga14 transcription factor. Using this chimera in a GAL-reporter yeast strain, an ex...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:15:1:29
更新日期：2000-05-01 00:00:00
abstract：:Tall fescue plants are widely exposed to white grubs. Regarding the rate of damage caused by the white grubs to tall fescue and difficulty of its ecological and economical control, production of resistant cultivars is a priority. In this experiment, for the first time, we report production of transgenic lines resistan...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-019-00183-5
更新日期：2019-07-01 00:00:00
abstract：:A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02760858
更新日期：1998-10-01 00:00:00
abstract：:Expression of certain transgenes from an adenovirus vector can be deleterious to its own replication. This can result in the inhibition of virus rescue, reduced viral yields, or, in the worst case, make it impossible to construct a vector expressing the inhibiting transgene product. A gene regulation system based on t...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02686012
更新日期：2007-03-01 00:00:00
abstract：:Adeno-associated virus (AAV) is a small, non-enveloped virus used as vector in gene therapy, mainly produced in human cells and in baculovirus systems. Intense studies on these platforms led to the production of vectors with titers between 103 and 105 viral genomes (vg) per cells. In spite of this, vector yields need ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00294-4
更新日期：2021-02-01 00:00:00
abstract：:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9204-6
更新日期：2010-01-01 00:00:00
abstract：:Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technol...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:25:3:275
更新日期：2003-11-01 00:00:00
