Analysis of HOX gene expression patterns in human breast cancer.

abstract：:Alternaria blight caused by Alternaria brassicicola (Schwein.) Wiltshire and A. brassicae (Berk.) Sacc., is one of the most important disease of rapeseed-mustard, characterized by the formation of spots on leaves, stem, and siliquae with premature defoliation and stunting of growth. These two species are very difficul...

journal_title：Molecular biotechnology

authors： Singh R,Kumar S,Kashyap PL,Srivastava AK,Mishra S,Sharma AK

更新日期：2014-11-01 00:00:00

abstract：:Water-soluble quinoprotein glucose dehydrogenase (PQQGDH-B) is a dimeric enzyme whose application for glucose sensing is the focus of much attention. We attempted to increase the thermal stability of PQQGDH-B by introducing a disulfide bond at the dimer interface. The Ser residue at position 415 was selected for subst...

journal_title：Molecular biotechnology

authors： Igarashi S,Sode K

更新日期：2003-06-01 00:00:00

abstract：:The simple use of nonisotopic hybridization probes to detect complementary sequences provides valuable information in a large number of research and commercial applications. In hybridization assays, the four "S's (speed, simplicity, sensitivity, and specificity) are important criteria for determining the choice of pro...

journal_title：Molecular biotechnology

authors： Ruth JL

更新日期：1996-10-01 00:00:00

abstract：:Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...

journal_title：Molecular biotechnology

authors： Dabrowski S,Maciuńska J,Synowiecki J

更新日期：1998-12-01 00:00:00

abstract：:A new lipase gene designated as SlLipA was isolated from Serratia liquefaciens S33 DB-1 by the genomic-walking method. The cloned gene contained an open reading frame (ORF) of 1,845 bp encoding 615 amino acids with a conserved GXSXG motif. Genome sequence analysis showed that an aldo/keto reductase gene closed to the ...

journal_title：Molecular biotechnology

authors： Yao H,Yu S,Zhang L,Zuo K,Ling H,Zhang F,Tang K

更新日期：2008-02-01 00:00:00

abstract：:There is a growing pharmaceutical market for protein-based drugs for use in therapy and diagnosis. The rapid developments in molecular and cell biology have resulted in production of expression systems for manufacturing of recombinant proteins and monoclonal antibodies. These proteins are glycosylated when expressed i...

journal_title：Molecular biotechnology

authors： Nilsson B

更新日期：1994-12-01 00:00:00

abstract：:A functional assay for proteolytic processing of the amyloid precursor protein (APP) was set up in yeast. This consisted of a membrane-bound chimeric protein containing the beta-secretase cleaved C-terminal fragment of APP fused to the Ga14 transcription factor. Using this chimera in a GAL-reporter yeast strain, an ex...

journal_title：Molecular biotechnology

authors： Gunyuzlu PL,White WH,Davis GL,Hollis GF,Toyn JH

更新日期：2000-05-01 00:00:00

abstract：:Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achieveme...

journal_title：Molecular biotechnology

authors： Li R,Kang G,Hu M,Huang H

更新日期：2019-01-01 00:00:00

abstract：:Hereditary cancers account for approximately 10 % of breast and ovarian cancers. Mutations of the BRCA1 and BRCA2 genes, encoding two proteins involved in DNA repair, underlie most cases of such hereditary cancers. Women with BRCA mutations develop breast cancer in 50-80 % of cases and ovarian cancer in 10-40 % of cas...

journal_title：Molecular biotechnology

authors： Vuttariello E,Borra M,Calise C,Mauriello E,Greggi S,Vecchione A,Biffali E,Chiappetta G

更新日期：2013-07-01 00:00:00

abstract：:Lentivirus-mediated RNA interference (RNAi) is a potent experimental tool for investigating gene functions in vitro and in vivo. It has advantages that transgenic technology lacks. However, in vivo applications are difficult to apply in the central nervous system of non-model organisms due to the lack of a standard br...

journal_title：Molecular biotechnology

authors： Chen Q,Zhu T,Jones G,Zhang J,Sun Y

更新日期：2013-06-01 00:00:00

abstract：:A sensitive method for staining proteins after transfer from polyacrylamide gels to nitrocellulose paper is described. Transferred proteins are first derivatized by reaction of the nitrocellulose replica with sulfosuccinimidobiotin and are then reacted sequentially with streptavidin, rabbit anti-streptavidin, and hors...

journal_title：Molecular biotechnology

authors： Santora KE,Nelson SA,Lewis KA,LaRochelle WJ

更新日期：2000-06-01 00:00:00

abstract：:G-quartets are square planar arrangements of four guanine bases, which can form extraordinarily stable stacks when present in nucleic acid sequences. Such G-quadruplex structures were long regarded as an in vitro phenomenon, but the widespread presence of suitable sequences in genomes and the identification of protein...

journal_title：Molecular biotechnology

authors： Bryan TM,Baumann P

更新日期：2011-10-01 00:00:00

abstract：:Efficient display of antibody on filamentous phage M13 coat is crucial for successful biopanning selections. We applied a directed evolution strategy to improve the oligovalent display of a poorly behaving Fab fragment fused to phage gene-3 for minor coat protein (g3p). The Fab displaying clones were enriched from a r...

journal_title：Molecular biotechnology

authors： Huovinen T,Sanmark H,Ylä-Pelto J,Vehniäinen M,Lamminmäki U

更新日期：2010-03-01 00:00:00

abstract：:Internal ribosome entry site (IRES) elements are highly structured RNA sequences that function to recruit ribosomes for the initiation of translation. In contrast to the canonical cap-binding, the mechanism of IRES-mediated translation initiation is still poorly understood. Translation initiation of the coxsackievirus...

journal_title：Molecular biotechnology

authors： Souii A,Ben M'hadheb-Gharbi M,Gharbi J

更新日期：2013-10-01 00:00:00

abstract：:To develop combined vaccine systems, we have generated Edwardsiella tarda ghosts (ETG) displaying a foreign protein on the outer membrane and also Ed. tarda cadaver (ETC) expressing a heterologous protein in the cytoplasm. Green fluorescent protein (GFP) was used as a model foreign protein. A constitutive promoter (Et...

journal_title：Molecular biotechnology

authors： Choi SH,Nam YK,Kim KH

更新日期：2010-10-01 00:00:00

abstract：:A sandwich-type enzyme-linked immunosorbent assay (ELISA) was established for monitoring the secretion of ZZ-fusion proteins. Two antibodies, a monoclonal mouse anti-human proinsulin and a rabbit anti-bovine IgG (strongly binding to the ZZ-domain), were used to quantify the secretion of recombinant human ZZ-proinsulin...

journal_title：Molecular biotechnology

authors： Mergulhão FJ,Monteiro GA,Cabral JM,Taipa MA

更新日期：2001-11-01 00:00:00

abstract：:The major online single nucleotide polymorphism (SNP) databases freely available as research tools for genetic analysis are explained, reviewed, and compared. An outline is given of the search strategies that can be used with the most extensive current SNP databases: National Centre for Biotechnology Information (NCBI...

journal_title：Molecular biotechnology

authors： Phillips C

更新日期：2007-01-01 00:00:00

abstract：:Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ab...

journal_title：Molecular biotechnology

authors： Szurgot I,Jedynak M,Podsiadla-Bialoskorska M,Piwowarski J,Szolajska E,Chroboczek J

更新日期：2015-06-01 00:00:00

abstract：:The ability to identify disease-associated genes using microarray technology is dependent on isolating high-quality total RNA from the diseased tissue under study. However, it is not always possible to obtain large amounts of affected tissue from patients and therefore the quality and yield of RNA may be compromised. ...

journal_title：Molecular biotechnology

authors： Murray EJ

更新日期：2003-02-01 00:00:00

abstract：:Ageing is a complex and progressive phenomenon, during which the accumulation of morphological and chemical changes seriously compromises the capacity of the cells to proliferate and fulfil their biological tasks. The increase in the average age of the world population, associated with a higher occurrence of age-relat...

journal_title：Molecular biotechnology

authors： Tito A,Barbulova A,Zappelli C,Leone M,Ruvo M,Mercurio FA,Chambery A,Russo R,Colucci MG,Apone F

更新日期：2019-03-01 00:00:00

abstract：:Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher's disease, the most common inherited storage disorder. Treatment consists of enzyme replacement therapy by the administration of recombinant GCR produced in Chinese hamster ovary cells. The production of anti-GCR antibodies has already been...

journal_title：Molecular biotechnology

authors： Novo JB,Oliveira ML,Magalhães GS,Morganti L,Raw I,Ho PL

更新日期：2010-11-01 00:00:00

abstract：:The induction of several secondary metabolites in plants is one of the most commonly observed effects after the external addition of methyl jasmonate (MeJA). After the elicitation of Catharanthus roseus hairy roots with different concentrations of MeJA, changes in the accumulation of alkaloids such as ajmalicine, serp...

journal_title：Molecular biotechnology

authors： Ruiz-May E,Galaz-Avalos RM,Loyola-Vargas VM

更新日期：2009-03-01 00:00:00

abstract：:The rhoptry 2 protein (Rop2) is an interesting protein of Toxoplasma gondii that is involved in the parasite invasion of host cell, it has three T-cell epitopes and high antigenic value. However, the expression of Rop2 as a recombinant protein in Escherichia coli is not an easy task, showing low levels of expression o...

journal_title：Molecular biotechnology

authors： Nigro M,Martin V,Kaufer F,Carral L,Angel SO,Pszenny V

更新日期：2001-07-01 00:00:00

abstract：:Recombinant proteins are useful tools in biological research, drug development, and drug screening. Specially designed expression vectors have been developed to introduce cDNA for recombinant protein expression in mammalian cells. We have combined a dicistronic mRNA design for expression of the recombinant protein, us...

journal_title：Molecular biotechnology

authors： Pu H,Cashion LM,Kretschmer PJ,Liu Z

更新日期：1998-08-01 00:00:00

abstract：:We report a rapid and simple method for isolating the 5'-end of plant genes from genomic DNA by polymerase chain reaction (PCR) with TATA-box-based degenerate primers (TDPs). The TDPs were specially designed according to the TATA box, which is conserved in the promoter region of most plant genes. The unknown 5'-ends o...

journal_title：Molecular biotechnology

authors： Guo Y,Ma L,Ji Y,Pu G,Liu B,Du Z,Li G,Ye H,Wang H

更新日期：2011-02-01 00:00:00

abstract：:Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant geno...

journal_title：Molecular biotechnology

authors： Zvirin T,Magrisso L,Yaari A,Shoseyov O

更新日期：2018-06-01 00:00:00

abstract：:A total of 41 accessions of Aglianico belonging to three different biotypes (Taburno, Taurasi, and Vulture) and 9 accessions of Sirica grapes were sampled from diverse areas of Campania (Italy). All accessions were first genotyped using 21 microsatellite markers (SSR) to evaluate possible homonymies, synonymies, and t...

journal_title：Molecular biotechnology

authors： De Lorenzis G,Imazio S,Biagini B,Failla O,Scienza A

更新日期：2013-06-01 00:00:00

abstract：:As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...

journal_title：Molecular biotechnology

authors： Hitt DC,Booth JL,Dandapani V,Pennington LR,Gimble JM,Metcalf J

更新日期：2000-03-01 00:00:00

abstract：:This article presents a simple and rapid method for removal of unincorporated label and proteins from DNA sequencing reactions by using Wizard purification resin. This method can be successfully applied for preparation of end-labeled oligonucleotides free of unincorporated label, which is important in experiments (inc...

journal_title：Molecular biotechnology

authors： Kaczorowski T,Sektas M

更新日期：1996-04-01 00:00:00

abstract：:Identification of expressed sequence tags (ESTs) in large genomic segments is an important step in positional cloning and genomic mapping studies. A simple and efficient polymerase chain reaction (PCR)-based approach is described here to identify coding sequences in large genomic fragments of DNA cloned in vectors suc...

journal_title：Molecular biotechnology

authors： Parimoo S

更新日期：1997-12-01 00:00:00