Abstract:
:A sandwich-type enzyme-linked immunosorbent assay (ELISA) was established for monitoring the secretion of ZZ-fusion proteins. Two antibodies, a monoclonal mouse anti-human proinsulin and a rabbit anti-bovine IgG (strongly binding to the ZZ-domain), were used to quantify the secretion of recombinant human ZZ-proinsulin to the growth medium of Escherichia coli cultures. The method here reported conjugates the advantages of sandwich-type ELISA assays, namely, high sensitivity, specificity, and throughput, with the possibility of quantifying small protein molecules (e.g., peptides). A further advantage of gene fusion techniques integrating both downstream processing and product detection and quantitation is highlighted. The method is capable of detecting levels of 0.05 ng of ZZ-proinsulin.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Mergulhão FJ,Monteiro GA,Cabral JM,Taipa MAdoi
10.1385/MB:19:3:239keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
239-44issue
3eissn
1073-6085issn
1559-0305pii
MB:19:3:239journal_volume
19pub_type
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