Enhanced protein secretion from insect cells by co-expression of the chaperone calreticulin and translation initiation factor eIF4E.

abstract：:Murraya koenigii (L.) Spreng. (Rutaceae), is an aromatic plant and much valued for its flavor, nutritive and medicinal properties. In this study, three DNA fingerprinting methods viz., random amplification of polymorphic DNA (RAPD), directed amplification of minisatellite DNA (DAMD), and inter-simple sequence repeat (...

journal_title：Molecular biotechnology

authors： Verma S,Rana TS

更新日期：2013-02-01 00:00:00

abstract：:Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases ...

journal_title：Molecular biotechnology

authors： Dimopoulou M,Claisse O,Dutilh L,Miot-Sertier C,Ballestra P,Lucas PM,Dols-Lafargue M

更新日期：2017-08-01 00:00:00

abstract：:RNA editing is one of the post-transcriptional RNA processes. RNA editing generates RNA and protein diversity in eukaryotes and results in specific amino acid substitutions, deletions, and changes in gene expression levels. Adenosine-to-inosine RNA editing represents the most important class of editing in human and af...

journal_title：Molecular biotechnology

authors： Tang W,Fei Y,Page M

更新日期：2012-09-01 00:00:00

abstract：:Hereditary cancers account for approximately 10 % of breast and ovarian cancers. Mutations of the BRCA1 and BRCA2 genes, encoding two proteins involved in DNA repair, underlie most cases of such hereditary cancers. Women with BRCA mutations develop breast cancer in 50-80 % of cases and ovarian cancer in 10-40 % of cas...

journal_title：Molecular biotechnology

authors： Vuttariello E,Borra M,Calise C,Mauriello E,Greggi S,Vecchione A,Biffali E,Chiappetta G

更新日期：2013-07-01 00:00:00

abstract：:This article describes the construction, expression, and purification of RNase single-chain antibody fusion proteins. To construct a fusion protein, the gene for each moiety, the RNase and the binding ligand, is modified separately to contain complementary DNA encoding a 13 amino acid spacer that separates the RNase f...

journal_title：Molecular biotechnology

authors： Newton DL,Rybak SM

更新日期：2002-01-01 00:00:00

abstract：:Adeno-associated virus (AAV) is a small, non-enveloped virus used as vector in gene therapy, mainly produced in human cells and in baculovirus systems. Intense studies on these platforms led to the production of vectors with titers between 103 and 105 viral genomes (vg) per cells. In spite of this, vector yields need ...

journal_title：Molecular biotechnology

authors： Galli A,Iaia I,Milella MS,Cipriani F,Della Latta V,Giacca M,Zentilin L,Cervelli T

更新日期：2021-02-01 00:00:00

abstract：:Alternaria blight caused by Alternaria brassicicola (Schwein.) Wiltshire and A. brassicae (Berk.) Sacc., is one of the most important disease of rapeseed-mustard, characterized by the formation of spots on leaves, stem, and siliquae with premature defoliation and stunting of growth. These two species are very difficul...

journal_title：Molecular biotechnology

authors： Singh R,Kumar S,Kashyap PL,Srivastava AK,Mishra S,Sharma AK

更新日期：2014-11-01 00:00:00

abstract：:Terminal Restriction Fragment Length Polymorphism (T-RFLP) or Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (FluRFLP) have made a significant impact on the way in which PCR products amplified from mixed community DNA extracts have been assessed. Technically, these approaches are essent...

journal_title：Molecular biotechnology

authors： Bruce KD,Hughes MR

更新日期：2000-11-01 00:00:00

abstract：:Transgenic rice seed can be utilized as a bioreactor to produce high-value recombinant proteins. Mouse interleukin 4 (mIL-4) and mIL-6 were specifically expressed as secretory proteins in rice endosperm by ligating the N-terminal glutelin B-1 (GluB-1) signal peptide and the C-terminal KDEL endoplasmic reticulum retent...

journal_title：Molecular biotechnology

authors： Fujiwara Y,Yang L,Takaiwa F,Sekikawa K

更新日期：2016-04-01 00:00:00

abstract：:In this study, human alpha-1,4-N-acetylglucosaminyltransferase (alpha4GnT) fused with GFP(uv) (GFP(uv)-alpha4GnT) was expressed using both a transformed cell system and silkworm larvae. A Tn-pXgp-GFP(uv)-alpha4GnT cell line, isolated after expression vector transfection, produced 106 mU/ml of alpha4GnT activity in sus...

journal_title：Molecular biotechnology

authors： Nakajima M,Kato T,Kanamasa S,Park EY

更新日期：2009-09-01 00:00:00

abstract：:Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achieveme...

journal_title：Molecular biotechnology

authors： Li R,Kang G,Hu M,Huang H

更新日期：2019-01-01 00:00:00

abstract：:Compounds based on a flavonoid (di-phenolic) ring structure are emerging as a potentially important new class of pharmaceutical compounds with a broad range of biological activities, most prominent of which are their potential role as anticancer agents. These compounds exert a wide range of upregulating and downregula...

journal_title：Molecular biotechnology

authors： Brown DM,Kelly GE,Husband AJ

更新日期：2005-07-01 00:00:00

abstract：:Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...

journal_title：Molecular biotechnology

authors： Tülek A,Karataş E,Çakar MM,Aydın D,Yılmazcan Ö,Binay B

更新日期：2021-01-01 00:00:00

abstract：:In this study, we investigated the relative expression of the Rous sarcoma virus (RSV) promoter-driven expression of enhanced green fluorescent protein (EGFP) in fibroblasts of transgenic quails. We analyzed the direct influence of CpG methylation of the RSV promoter on the transcriptional activity of delivered transg...

journal_title：Molecular biotechnology

authors： Jang HJ,Choi JW,Kim YM,Shin SS,Lee K,Han JY

更新日期：2011-11-01 00:00:00

abstract：:In this review I briefly describe the technique of protein engineering and indicate how the present state of knowledge allows proteins to be mutated to increase or decrease stability. I discuss experiments on both model proteins and those of relevance to the food industry and show how hydrophobic forces are a major dr...

journal_title：Molecular biotechnology

authors： Goodenough PW

更新日期：1995-10-01 00:00:00

abstract：:PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...

journal_title：Molecular biotechnology

authors： Lorenzo V,Mascanzoni F,Vitagliano L,Ruvo M,Doti N

更新日期：2016-05-01 00:00:00

abstract：:Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...

journal_title：Molecular biotechnology

authors： Madonna R,Delli Pizzi S,Tartaro A,De Caterina R

更新日期：2014-05-01 00:00:00

abstract：:Considering the fact that Prays oleae is one of the most pathogenic insects to the olive tree in the Mediterranean basin, particularly in Tunisia, the mode of action of Cry insecticidal toxins of Bacillus thuringiensis kurstaki in Prays oleae midgut was investigated. The proteolysis of Bacillus thuringiensis delta-end...

journal_title：Molecular biotechnology

authors： Rouis S,Chakroun M,Saadaoui I,Jaoua S

更新日期：2007-02-01 00:00:00

abstract：:A functional assay for proteolytic processing of the amyloid precursor protein (APP) was set up in yeast. This consisted of a membrane-bound chimeric protein containing the beta-secretase cleaved C-terminal fragment of APP fused to the Ga14 transcription factor. Using this chimera in a GAL-reporter yeast strain, an ex...

journal_title：Molecular biotechnology

authors： Gunyuzlu PL,White WH,Davis GL,Hollis GF,Toyn JH

更新日期：2000-05-01 00:00:00

abstract：:The advent of recombinant antibody technology led to an enormous revival in the use of antibodies as diagnostic and therapeutic tools for fighting cancer. This review provides a brief historical sketch of the development of recombinant antibodies for the diagnosis and immunotherapy of cancer and summarizes the most si...

journal_title：Molecular biotechnology

authors： Krauss J

更新日期：2003-09-01 00:00:00

abstract：:The biosensor field has grown enormously since the first demonstration of the biosensor concept by Leland C. Clark, Jr. in 1962. Today's biosensor market is dominated by glucose biosensors, mass-produced enzyme electrodes for the rapid self-diagnosis of blood glucose levels by diabetes sufferers. Here we take a histor...

journal_title：Molecular biotechnology

authors： Newman JD,Setford SJ

更新日期：2006-03-01 00:00:00

abstract：:Cyclooxygenase (COX)-2 plays an important role in the development of cancer and has been recognized as a potential therapeutic target. Because nonsteroidal anti-inflammatory drugs (NSAIDs) are able to inhibit the activity of this enzyme, the potential efficacy of such drugs for purposes of cancer prevention or therapy...

journal_title：Molecular biotechnology

authors： Liu YT,Kardosh A,Cooc J,Schönthal AH

更新日期：2006-11-01 00:00:00

abstract：:The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...

journal_title：Molecular biotechnology

authors： Kulichkova VA,Artamonova TO,Zaykova JJ,Ermolaeva JB,Khodorkovskii MA,Barlev NA,Tomilin AN,Tsimokha AS

更新日期：2015-01-01 00:00:00

abstract：:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...

journal_title：Molecular biotechnology

authors： Bekli S,Aktas B,Gencer D,Aslim B

更新日期：2019-09-01 00:00:00

abstract：:Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...

journal_title：Molecular biotechnology

authors： Arneaud SL,Porter JR

更新日期：2015-12-01 00:00:00

abstract：:Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study, a thermostable pectin methylesterase (CtPME) from Clostridium thermocellum belonging to family 8 carbohydrate...

journal_title：Molecular biotechnology

authors： Rajulapati V,Goyal A

更新日期：2017-05-01 00:00:00

abstract：:Translation initiation of Coxsackievirus B3 (CVB3) RNA is directed by an internal ribosome entry site (IRES) within the 5' untranslated region. Host cell factors involved in this process include some canonical translation factors and additional RNA-binding proteins. We have, previously, described that the Sabin3-like ...

journal_title：Molecular biotechnology

authors： Souii A,M'hadheb-Gharbi MB,Aouni M,Gharbi J

更新日期：2013-06-01 00:00:00

abstract：:Recombinant proteins are useful tools in biological research, drug development, and drug screening. Specially designed expression vectors have been developed to introduce cDNA for recombinant protein expression in mammalian cells. We have combined a dicistronic mRNA design for expression of the recombinant protein, us...

journal_title：Molecular biotechnology

authors： Pu H,Cashion LM,Kretschmer PJ,Liu Z

更新日期：1998-08-01 00:00:00

abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...

journal_title：Molecular biotechnology

authors： do Nascimento NC,Menguer PK,Sperotto RA,de Almeida MR,Fett-Neto AG

更新日期：2013-05-01 00:00:00

abstract：:Human papillomavirus (HPV) is the causal agent of cervical cancer, one of the most common causes of death in women worldwide, and its E7 antigen is the major candidate for a therapeutic vaccine. The large scale production of E7 by molecular farming that would lead to the development of a safe and inexpensive vaccine i...

journal_title：Molecular biotechnology

authors： Morgenfeld M,Lentz E,Segretin ME,Alfano EF,Bravo-Almonacid F

更新日期：2014-11-01 00:00:00