Abstract:
:Transgenic rice seed can be utilized as a bioreactor to produce high-value recombinant proteins. Mouse interleukin 4 (mIL-4) and mIL-6 were specifically expressed as secretory proteins in rice endosperm by ligating the N-terminal glutelin B-1 (GluB-1) signal peptide and the C-terminal KDEL endoplasmic reticulum retention signal under control of the endosperm-specific GluB-1 promoter. In the transgenic rice seed, mIL-4 and mIL-6 accumulated in levels up to 0.43 mg/g grain and 0.16 mg/g grain, respectively. The reducing agents and detergents required for extraction from the transgenic rice seeds differed between the two proteins, indicating differences in their intracellular localization within the endosperm cell. Purified mIL-4 and mIL-6 exhibited high activity and very low endotoxin contamination.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Fujiwara Y,Yang L,Takaiwa F,Sekikawa Kdoi
10.1007/s12033-016-9920-7subject
Has Abstractpub_date
2016-04-01 00:00:00pages
223-31issue
4eissn
1073-6085issn
1559-0305pii
10.1007/s12033-016-9920-7journal_volume
58pub_type
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