Biological significance of RNA editing in cells.

abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...

journal_title：Molecular biotechnology

authors： Novo C,Domingos A,Karmali A

更新日期：2001-02-01 00:00:00

abstract：:Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...

journal_title：Molecular biotechnology

authors： Díaz-Dinamarca DA,Jerias JI,Soto DA,Soto JA,Díaz NV,Leyton YY,Villegas RA,Kalergis AM,Vásquez AE

更新日期：2018-03-01 00:00:00

abstract：:Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadv...

journal_title：Molecular biotechnology

authors： Christensen AC

更新日期：2001-03-01 00:00:00

abstract：:Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utiliz...

journal_title：Molecular biotechnology

authors： Wang X,Tian W,Li Y

更新日期：2008-01-01 00:00:00

abstract：:In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of ...

journal_title：Molecular biotechnology

authors： Takan JP,Chipili J,Muthumeenakshi S,Talbot NJ,Manyasa EO,Bandyopadhyay R,Sere Y,Nutsugah SK,Talhinhas P,Hossain M,Brown AE,Sreenivasaprasad S

更新日期：2012-02-01 00:00:00

abstract：:Jet-injection has become an applicable technology among other established nonviral delivery systems, such as particle bombardment or in vivo electroporation. The low-volume jet injector employed in this study uses compressed air to inject solutions of 1.5-10 microL containing naked DNA into the desired tissue. The nov...

journal_title：Molecular biotechnology

authors： Walther W,Stein U,Fichtner I,Voss C,Schmidt T,Schleef M,Nellessen T,Schlag PM

更新日期：2002-06-01 00:00:00

abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...

journal_title：Molecular biotechnology

authors： Yang Y,Wang Q,Guo D

更新日期：2008-09-01 00:00:00

abstract：:Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...

journal_title：Molecular biotechnology

authors： Madonna R,Delli Pizzi S,Tartaro A,De Caterina R

更新日期：2014-05-01 00:00:00

abstract：:Costimulatory signal(s) in addition to engagement of T cell receptor is important for optimal activation of T lymphocytes. During last decade several dozens of new costimulatory molecules, including 4-1BB, CD40, OX40, CD27, and ICOS, have been identified. It is likely that in Post-Genome Era more molecules with potent...

journal_title：Molecular biotechnology

authors： Chapoval AI,Zhu G,Chen L

更新日期：2002-07-01 00:00:00

abstract：:The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organ...

journal_title：Molecular biotechnology

authors： Gatter M,Gatter T,Matthäus F

更新日期：2012-10-01 00:00:00

abstract：:Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic analyses of the gut of C. curvignathus revealed...

journal_title：Molecular biotechnology

authors： Woon JS,King PJH,Mackeen MM,Mahadi NM,Wan Seman WMK,Broughton WJ,Abdul Murad AM,Abu Bakar FD

更新日期：2017-07-01 00:00:00

abstract：:Recombinant adenovirus vectors have proven to be useful tools in facilitating gene transfer. Construction of such vectors requires a knowledge of the adenovirus genome structure and its life cycle. A commonly used recombinant adenovirus involves deletion of the E1 region; such a recombinant is traditionally produced b...

journal_title：Molecular biotechnology

authors： Davis AR,Wivel NA,Palladino JL,Tao L,Wilson JM

更新日期：2001-05-01 00:00:00

abstract：:The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...

journal_title：Molecular biotechnology

authors： Devon RS,Brookes AJ

更新日期：1996-06-01 00:00:00

abstract：:The significance of actin cytoskeleton on cell growth was historically studied using toxic drugs, such as cytochalasin. However, it is possible that unpredictable effects of these agents may have influenced the reported observations. In our study, we have established a drug-free system using cofilin overexpression to ...

journal_title：Molecular biotechnology

authors： Lee YJ,Keng PC

更新日期：2005-09-01 00:00:00

abstract：:The recombinant acidstable α-amylase (Ba-amy) of acidophilic bacterium Bacillus acidicola TSAS1 has been produced extracellularly using a combination of cloning (E. coli and P. pastoris) and physico-chemical treatment strategies. A total of 150,000 U/L of Ba-amy were attained under constitutive promoter in P. pastoris...

journal_title：Molecular biotechnology

authors： Parashar D,Satyanarayana T

更新日期：2016-11-01 00:00:00

abstract：:The induction of several secondary metabolites in plants is one of the most commonly observed effects after the external addition of methyl jasmonate (MeJA). After the elicitation of Catharanthus roseus hairy roots with different concentrations of MeJA, changes in the accumulation of alkaloids such as ajmalicine, serp...

journal_title：Molecular biotechnology

authors： Ruiz-May E,Galaz-Avalos RM,Loyola-Vargas VM

更新日期：2009-03-01 00:00:00

abstract：:In the context of clinical gene transfer using viral vectors, the risk of memory antivector immunity is often poorly appreciated. The immunological past of the patient, the site of injection, and the vector dose will play intertwined and decisive roles in the safety and efficacy of treatment. To circumvent the drawbac...

journal_title：Molecular biotechnology

authors： Perreau M,Kremer EJ

更新日期：2006-10-01 00:00:00

abstract：:L-Cysteine desulfhydrase (DES; EC 4.4.1.1) is the most important enzyme that catalyzes the decomposition of L-cysteine to pyruvate, ammonia, and hydrogen sulfide (H2S), the latter of which has recently been recognized as the third gasotransmitter for multiple signaling events in plants. Previous results showed the exi...

journal_title：Molecular biotechnology

authors： Xie Y,Lai D,Mao Y,Zhang W,Shen W,Guan R

更新日期：2013-07-01 00:00:00

abstract：:Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches h...

journal_title：Molecular biotechnology

authors： Morokuma D,Xu J,Hino M,Mon H,Merzaban JS,Takahashi M,Kusakabe T,Lee JM

更新日期：2017-05-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...

journal_title：Molecular biotechnology

authors： Hristodorov D,Fischer R,Joerissen H,Müller-Tiemann B,Apeler H,Linden L

更新日期：2013-03-01 00:00:00

abstract：:In this review I briefly describe the technique of protein engineering and indicate how the present state of knowledge allows proteins to be mutated to increase or decrease stability. I discuss experiments on both model proteins and those of relevance to the food industry and show how hydrophobic forces are a major dr...

journal_title：Molecular biotechnology

authors： Goodenough PW

更新日期：1995-10-01 00:00:00

abstract：:DNA damage is one of the most impactful events in living organisms, leading to DNA sequence changes (mutation) and disruption of biological processes. A study has identified a protein called Damage Suppressor Protein (Dsup) in the tardigrade Ramazzotius varieornatus that has shown to reduce the effects of radiation da...

journal_title：Molecular biotechnology

authors： Kirke J,Jin XL,Zhang XH

更新日期：2020-12-01 00:00:00

abstract：:Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...

journal_title：Molecular biotechnology

authors： Dabrowski S,Maciuńska J,Synowiecki J

更新日期：1998-12-01 00:00:00

abstract：:The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...

journal_title：Molecular biotechnology

authors： Long C,Cheng Y,Cui J,Liu J,Gan L,Zeng B,Long M

更新日期：2018-01-01 00:00:00

abstract：:The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...

journal_title：Molecular biotechnology

authors： Kulichkova VA,Artamonova TO,Zaykova JJ,Ermolaeva JB,Khodorkovskii MA,Barlev NA,Tomilin AN,Tsimokha AS

更新日期：2015-01-01 00:00:00

abstract：:A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...

journal_title：Molecular biotechnology

authors： Wang TT,Cheng WC,Lee BH

更新日期：1998-10-01 00:00:00

abstract：:Sample preparation is crucial for obtaining high-quality proteins for the purpose of electrophoretic separation and further analysis from tissues that contain high levels of interfering compounds. Hypericum perforatum is a medicinal plant that contains high amounts of phenolic compounds, of which hypericins, hyperfori...

journal_title：Molecular biotechnology

authors： Karppinen K,Taulavuori E,Hohtola A

更新日期：2010-11-01 00:00:00

abstract：:Brn-3a, a member of the POU gene family (so-called because of the similarity with the group of transcription factors Pit, Oct, and Unc), was found in neuronal cells engaged in the transcription activity of the p1 and p2 promoters of the most powerful antiapoptotic gene, namely, Bcl-2. The alternative splicing of Brn-3...

journal_title：Molecular biotechnology

authors： Chiarugi V,Del Rosso M,Magnelli L

更新日期：2002-10-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field ...

journal_title：Molecular biotechnology

authors： Jurinke C,Oeth P,van den Boom D

更新日期：2004-02-01 00:00:00

abstract：:A synthetic gene encoding a single chain Fv fragment of an antibody directed against the nuclear inclusion a (NIa) protein of potato virus Y (PVY) was used to transform two commercial potato cultivars (Claustar and BF15). The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavag...

journal_title：Molecular biotechnology

authors： Gargouri-Bouzid R,Jaoua L,Rouis S,Saïdi MN,Bouaziz D,Ellouz R

更新日期：2006-06-01 00:00:00