Abstract:
:The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organism and the option to introduce restriction enzyme target sites of choice. C.U.R.R.F. (Codon Usage regarding Restriction Finder) is a free Java(®)-based software program which is able to detect possible restriction sites in both coding and non-coding DNA sequences by introducing multiple silent or non-silent mutations, respectively. The deviation of an alternative sequence containing a desired restriction motive from the sequence with the optimal codon usage is considered during the search of potential restriction sites in coding DNA and mRNA sequences as well as protein sequences. C.U.R.R.F is available at http://www.zvm.tu-dresden.de/die_tu_dresden/fakultaeten/fakultaet_mathematik_und_naturwissenschaften/fachrichtung_biologie/mikrobiologie/allgemeine_mikrobiologie/currf.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Gatter M,Gatter T,Matthäus Fdoi
10.1007/s12033-011-9479-2subject
Has Abstractpub_date
2012-10-01 00:00:00pages
123-8issue
2eissn
1073-6085issn
1559-0305journal_volume
52pub_type
杂志文章abstract::Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9531-x
更新日期:2013-03-01 00:00:00
abstract::The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia A...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9423-5
更新日期:2012-03-01 00:00:00
abstract::A sandwich-type enzyme-linked immunosorbent assay (ELISA) was established for monitoring the secretion of ZZ-fusion proteins. Two antibodies, a monoclonal mouse anti-human proinsulin and a rabbit anti-bovine IgG (strongly binding to the ZZ-domain), were used to quantify the secretion of recombinant human ZZ-proinsulin...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:19:3:239
更新日期:2001-11-01 00:00:00
abstract::A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vect...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:32:2:093
更新日期:2006-02-01 00:00:00
abstract::Association studies using genome scans to identify quantitative trait loci for multifactorial disorders, with anything approaching reasonable power, have been compromised by the need for a very dense array of genetic markers and large numbers of affected individuals. These requirements impose enormous burdens on the g...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/mb:22:3:253
更新日期:2002-11-01 00:00:00
abstract::Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02740841
更新日期:1998-12-01 00:00:00
abstract::In this study cationic β-cyclodextrin-chitosan-mediated nanoparticles were used to transfer pmCherry-C1 into glioblastoma cells and their transfection efficiency were compared to lipofectamine and electroporation. Physicochemical characteristics of nanoparticles were evaluated by photon correlation spectroscopy and sc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9927-0
更新日期:2016-04-01 00:00:00
abstract::Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789331
更新日期:1995-06-01 00:00:00
abstract::In this study, human alpha-1,4-N-acetylglucosaminyltransferase (alpha4GnT) fused with GFP(uv) (GFP(uv)-alpha4GnT) was expressed using both a transformed cell system and silkworm larvae. A Tn-pXgp-GFP(uv)-alpha4GnT cell line, isolated after expression vector transfection, produced 106 mU/ml of alpha4GnT activity in sus...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9174-8
更新日期:2009-09-01 00:00:00
abstract::D-Allulose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity. Using D-psicose epimerase and L-rhamnulose kinase, a method enabling high conversio...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00174-6
更新日期:2019-06-01 00:00:00
abstract::Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to oth...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9384-8
更新日期:2011-10-01 00:00:00
abstract::As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:14:3:197
更新日期:2000-03-01 00:00:00
abstract::Immobilized-metal-ion affinity chromatography (IMAC) is used extensively for phosphopeptide enrichment in phosphoproteomics. However, the effect of nucleic acids in protein samples on phosphopeptide enrichment by IMAC has not yet been well clarified. In this study, we demonstrate that IMAC beads possess a strong adsor...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9176-6
更新日期:2009-09-01 00:00:00
abstract::Tall fescue plants are widely exposed to white grubs. Regarding the rate of damage caused by the white grubs to tall fescue and difficulty of its ecological and economical control, production of resistant cultivars is a priority. In this experiment, for the first time, we report production of transgenic lines resistan...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00183-5
更新日期:2019-07-01 00:00:00
abstract::In order to further study the B subunit of the Escherichia coli heat-labile enterotoxin (LTB), we obtained the LTB gene from pathogenic E. coli, cloned it into the pET22b (+) prokaryotic expression vector, and expressed it as a fusion protein with His tag in E. coli BL21 (DE3). The recombinant LTB was expressed and pu...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9101-4
更新日期:2009-02-01 00:00:00
abstract::The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:24:3:229
更新日期:2003-07-01 00:00:00
abstract::Tumor necrosis factor (TNF) is a central cytokine in the pathogenesis of septic shock and other inflammatory states. Assay by immunoassay is convenient, but, because of circulating soluble receptors, does not accurately reflect biological activity of the cytokine. This article describes how to perform a bioassay for T...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:15:3:243
更新日期:2000-07-01 00:00:00
abstract::Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00281-9
更新日期:2021-01-01 00:00:00
abstract::D-Stereospecific amidohydrolase (DAH) from Streptomyces sp. 82F2 has potential utility for the synthesis of D/L configuration dipeptides by an aminolysis reaction. Structural comparison of DAH with substrate-bound D-amino acid amidase revealed that three residues located in the active site pocket of DAH (Thr145, Ala26...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0104-5
更新日期:2018-09-01 00:00:00
abstract::NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expans...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00250-2
更新日期:2020-05-01 00:00:00
abstract::An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could repr...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9516-9
更新日期:2013-01-01 00:00:00
abstract::Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials us...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:21:1:057
更新日期:2002-05-01 00:00:00
abstract::Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9923-4
更新日期:2016-04-01 00:00:00
abstract::The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0046-3
更新日期:2018-01-01 00:00:00
abstract::Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:20:2:137
更新日期:2002-02-01 00:00:00
abstract::Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last y...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-011-9402-x
更新日期:2011-11-01 00:00:00
abstract::Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant geno...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0075-6
更新日期:2018-06-01 00:00:00
abstract::The use of synthetic genes may constitute a successful approach for the heterologous production and functional expression of bacterial antimicrobial peptides (bacteriocins) by recombinant yeasts. In this work, synthetic genes with adapted codon usage designed from the mature amino acid sequence of the bacteriocin ente...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9731-7
更新日期:2014-06-01 00:00:00
abstract::Genetically engineered animal models have been and will continue to be invaluable for exploring the basic mechanisms involved in the aging process as well as in extending our understanding of diseases found to be more prevalent in the older human population. Continued development of such in vivo systems will allow sci...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:19:1:045
更新日期:2001-09-01 00:00:00
abstract::Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:13:2:165
更新日期:1999-12-01 00:00:00