Abstract:
:A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vector and was under the control of the ubiquitin promoter and transformed into tobacco plants by Agrobacterium-mediated transformation. Transgenic plantlets were selected in a medium supplemented with 5 mg/L PPT. Polymerase chain reaction analysis confirmed stable integration of the synthetic CTB gene into a chromosomal DNA. A high level of CTB (1.8% of total soluble protein) was expressed in transgenic plants, which was 18-fold higher than that under the control of the expressed CaMV 35S promoter with native gene. The transgenic plants when transferred to a greenhouse proved to be resistant to 2% PPT.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Kang TJ,Kim BG,Yang JY,Yang MSdoi
10.1385/MB:32:2:093keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
93-100issue
2eissn
1073-6085issn
1559-0305pii
MB:32:2:093journal_volume
32pub_type
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