Abstract：
:The two asparagine-linked glycosylation sites of recombinant coagulation factor VIIa have been characterized by glycosidase digestions, size-exclusion chromatography (SEC), and mass spectrometry (MS). Nine structures were characterized as core fucosylated bi-and triantennary structures with 0-3 sialic-acid residues which were alpha 2-3 linked to galactose exclusively. Three of the structures had one or two galactose residues substituted by N-acetylgalactosamine. Significant differences were found between the oligosaccharide profiles for the two glycosylation sites in rFVIIa. At Asn322, the degree of sialylation was lower and higher amounts of structures containing N-acetylgalactosamine were found compared to Asn145.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Klausen NK,Bayne S,Palm Lsubject
Has Abstractpub_date
1998-06-01 00:00:00pages
195-204issue
3eissn
1073-6085issn
1559-0305journal_volume
9pub_type
杂志文章abstract：:Clinical molecular genetics has recently become recognized as a diagnostic discipline. This article covers the evolution, structure, and possible forward development of clinical molecular genetics. Topics covered include general test categories, introducing new tests, laboratory facilities, staffing and training, and ...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02752254
更新日期：1997-10-01 00:00:00
abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9066-3
更新日期：2008-09-01 00:00:00
abstract：:The advent of recombinant antibody technology led to an enormous revival in the use of antibodies as diagnostic and therapeutic tools for fighting cancer. This review provides a brief historical sketch of the development of recombinant antibodies for the diagnosis and immunotherapy of cancer and summarizes the most si...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:25:1:1
更新日期：2003-09-01 00:00:00
abstract：:Genes are recognized as undergoing genomic imprinting when they are capable of being expressed only from the paternal or only from the maternal chromosome. The process can occur coordinately within large physical domains in mammalian chromosomes. One interesting facet of the study of genomic imprinting is that it offe...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02915809
更新日期：1999-04-01 00:00:00
abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789104
更新日期：1995-04-01 00:00:00
abstract：:The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0080-9
更新日期：2018-05-01 00:00:00
abstract：:The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9799-0
更新日期：2015-01-01 00:00:00
abstract：:The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes needed for efficient solubilization of native cellulose, the major component of plants. In this work, we reported the molecular characterization of an important glycosyl-hydrolase enzyme classified as endo-β-1,4-glucanase. ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9714-0
更新日期：2014-04-01 00:00:00
abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:17:2:119
更新日期：2001-02-01 00:00:00
abstract：:Human adipose tissue-derived stromal cells (ADSCs) are being evaluated for cardiovascular repair. We developed an ex vivo method for producing angiogenic ADSCs transduced with a self-inactivating lentiviral vector (LV) expressing the enhanced green fluorescence protein (EGFP) from an internal cytomegalovirus (CMV) pro...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9537-4
更新日期：2013-05-01 00:00:00
abstract：:A shuttle vector pZL1 which can replicate both in Gluconobacter oxydans and Escherichia coli was constructed based on G. oxydans DSM2003 cryptic plasmid pGOX3, a homology of G. oxydans 621H pGOX3, and E. coli cloning vector pUC18. It was found to be stably maintained in G. oxydans during the serial subcultures in the ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9293-2
更新日期：2010-11-01 00:00:00
abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9546-3
更新日期：2013-05-01 00:00:00
abstract：:Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ab...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9850-9
更新日期：2015-06-01 00:00:00
abstract：:Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9888-8
更新日期：2015-12-01 00:00:00
abstract：:In mammals, CpG methylation is one of the mechanisms of epigenetic control over the linear sequence of bases of deoxyribonucleic acid (DNA); about 70% of CpG dinucleotides are methylated. The actual signal that triggers DNA methylation is not known, although repetitive DNA has been shown to be an attractive template f...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:26:3:215
更新日期：2004-03-01 00:00:00
abstract：:Despite the known importance of the composition of culture media and culture conditions on Bacillus thuringiensis growth and toxicity, very few reviews are concerned with this subject. This article reviews some aspects of the microbiology of Bacillus thuringiensis, and how toxicity is affected by the composition of gr...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02907471
更新日期：1995-08-01 00:00:00
abstract：:Characterization of novel plant gene promoters underpins the development of transgenic crop plants. Here, we report a novel 5' regulatory sequence (Eriubi D7) of the ubiquitin gene from Erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. A 3.2-kb regulatory sequence of u...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9875-0
更新日期：2015-09-01 00:00:00
abstract：:Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant geno...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0075-6
更新日期：2018-06-01 00:00:00
abstract：:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-019-00194-2
更新日期：2019-09-01 00:00:00
abstract：:The recombinant acidstable α-amylase (Ba-amy) of acidophilic bacterium Bacillus acidicola TSAS1 has been produced extracellularly using a combination of cloning (E. coli and P. pastoris) and physico-chemical treatment strategies. A total of 150,000 U/L of Ba-amy were attained under constitutive promoter in P. pastoris...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9970-x
更新日期：2016-11-01 00:00:00
abstract：:Indirubin is a strong inhibitor of several eukaryotic cell signaling pathways and shows promise as a treatment for myelocytic leukemia and Alzheimer's disease. The tmoABCDEF operon, encoding the components of a novel toluene 4-monooxygenase from the paint factory soil isolate, Pseudomonas sp. M4, was cloned and expres...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9863-4
更新日期：2015-08-01 00:00:00
abstract：:The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0046-3
更新日期：2018-01-01 00:00:00
abstract：:The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02900362
更新日期：1996-06-01 00:00:00
abstract：:D-Stereospecific amidohydrolase (DAH) from Streptomyces sp. 82F2 has potential utility for the synthesis of D/L configuration dipeptides by an aminolysis reaction. Structural comparison of DAH with substrate-bound D-amino acid amidase revealed that three residues located in the active site pocket of DAH (Thr145, Ala26...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0104-5
更新日期：2018-09-01 00:00:00
abstract：:Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0094-3
更新日期：2018-08-01 00:00:00
abstract：:In an effort to develop microalgae as a robust system for the production of valuable proteins, we analyzed some of the factors affecting recombinant protein expression in the chloroplast of the green alga Chlamydomonas reinhardtii. We monitored mRNA accumulation, protein synthesis, and protein turnover for three codon...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9348-4
更新日期：2011-05-01 00:00:00
abstract：:Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utiliz...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-0073-6
更新日期：2008-01-01 00:00:00
abstract：:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9204-6
更新日期：2010-01-01 00:00:00
abstract：:A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vect...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:32:2:093
更新日期：2006-02-01 00:00:00
abstract：:Biobanks, more formally known as biological resource centers (BRCs), form an "unsung" yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and cell-lines would be effectively impossible. BRCs...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-008-9099-7
更新日期：2008-10-01 00:00:00
