Cloning of Toluene 4-Monooxygenase Genes and Application of Two-Phase System to the Production of the Anticancer Agent, Indirubin.

abstract：:Over the last decade transgenic mouse models have become a common experimental tool for unraveling gene function. During this time there has been a growing expectation that transgenes resemble the in vivo state as much as possible. To this end, a preference away from heterologous promoters has emerged, and transgene c...

journal_title：Molecular biotechnology

authors： Ristevski S

更新日期：2005-02-01 00:00:00

abstract：:Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to oth...

journal_title：Molecular biotechnology

authors： Japelaghi RH,Haddad R,Garoosi GA

更新日期：2011-10-01 00:00:00

abstract：:Tumor necrosis factor (TNF) is a central cytokine in the pathogenesis of septic shock and other inflammatory states. Assay by immunoassay is convenient, but, because of circulating soluble receptors, does not accurately reflect biological activity of the cytokine. This article describes how to perform a bioassay for T...

journal_title：Molecular biotechnology

authors： Evans TJ

更新日期：2000-07-01 00:00:00

abstract：:The Delta12 desaturase represents a diverse gene family in plants and is responsible for conversion of oleic acid (18:1) to linoleic acid (18:2). Several members of this family are known from plants like Arabidopsis and Soybean. Using primers from conserved C- and N-terminal regions, we have cloned a novel Delta12 des...

journal_title：Molecular biotechnology

authors： Khadake RM,Ranjekar PK,Harsulkar AM

更新日期：2009-06-01 00:00:00

abstract：:Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase ge...

journal_title：Molecular biotechnology

authors： Banerjee A,Dubey S,Kaul P,Barse B,Piotrowski M,Banerjee UC

更新日期：2009-01-01 00:00:00

abstract：:The GacS/A system in Azotobacter vinelandii regulates alginate and alkylresorcinols production through RsmZ1, a small regulatory RNA (sRNA) that releases the translational repression of the algD and arpR mRNAs caused by the RsmA protein. In the Pseudomonadaceae family, the Rsm-sRNAs are grouped into three families: Rs...

journal_title：Molecular biotechnology

authors： López-Pliego L,García-Ramírez L,Cruz-Gómez EA,Domínguez-Ojeda P,López-Pastrana A,Fuentes-Ramírez LE,Núñez C,Castañeda M

更新日期：2018-09-01 00:00:00

abstract：:Virus-like particles (VLPs) have vast potential for applications in nanoscience and nanomedicine. These biological nanoparticles may be used for medical imaging, vaccination, or tissue-specific delivery of drugs or other bioactive molecules. VLPs of Human parvovirus B19 (B19 V) can be assembled in vitro from the recom...

journal_title：Molecular biotechnology

authors： Sánchez-Rodríguez SP,Enrriquez-Avila JV,Soto-Fajardo JM,Peña-Montes C,Bustos-Jaimes I

更新日期：2015-04-01 00:00:00

abstract：:Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches h...

journal_title：Molecular biotechnology

authors： Morokuma D,Xu J,Hino M,Mon H,Merzaban JS,Takahashi M,Kusakabe T,Lee JM

更新日期：2017-05-01 00:00:00

abstract：:Pasteurella multocida is the main cause of haemorrhagic septicaemia (HS) outbreak in livestock, such as cattle and buffaloes. Conventional vaccines such as alum-precipitated or oil-adjuvant broth bacterins were injected subcutaneously to provide protection against HS. However, the immunity developed is only for short ...

journal_title：Molecular biotechnology

authors： Chelliah S,Velappan RD,Lim KT,Swee CWK,Nor Rashid N,Rothan HA,Kabir N,Ismail S

更新日期：2020-05-01 00:00:00

abstract：:Gene reporter systems play a key role in gene expression and regulation studies. This review describes the ideal reporter systems, including reporter expression vector design. It summarizes the many uses of genetic reporters and outlines the currently available and commonly used reporter systems. Each system is descri...

journal_title：Molecular biotechnology

authors： Schenborn E,Groskreutz D

更新日期：1999-11-01 00:00:00

abstract：:Lentivirus-mediated RNA interference (RNAi) is a potent experimental tool for investigating gene functions in vitro and in vivo. It has advantages that transgenic technology lacks. However, in vivo applications are difficult to apply in the central nervous system of non-model organisms due to the lack of a standard br...

journal_title：Molecular biotechnology

authors： Chen Q,Zhu T,Jones G,Zhang J,Sun Y

更新日期：2013-06-01 00:00:00

abstract：:The mapping of genetic loci within organisms has been accelerated by the advent of Radiation Hybrid (RH) panels. These panels are available for humans and non-humans including mice, baboon, rat, and canine. This article contains a general protocol for the use of the Genebridge 4 whole genome RH panel to map a human lo...

journal_title：Molecular biotechnology

authors： Friedman JS,Walter MA

更新日期：2001-10-01 00:00:00

abstract：:Genetically engineered animal models have been and will continue to be invaluable for exploring the basic mechanisms involved in the aging process as well as in extending our understanding of diseases found to be more prevalent in the older human population. Continued development of such in vivo systems will allow sci...

journal_title：Molecular biotechnology

authors： Andersen JK

更新日期：2001-09-01 00:00:00

abstract：:N-Succinyl-amino acid racemase (NSAAR), long referred to as N-acyl- or N-acetyl-amino acid racemase, is an enolase superfamily member whose biotechnological potential was discovered decades ago, due to its use in the industrial dynamic kinetic resolution methodology first known as "Acylase Process". In previous works,...

journal_title：Molecular biotechnology

authors： Soriano-Maldonado P,Andújar-Sánchez M,Clemente-Jiménez JM,Rodríguez-Vico F,Las Heras-Vázquez FJ,Martínez-Rodríguez S

更新日期：2015-05-01 00:00:00

abstract：:Papaya ringspot virus (PRSV) causes severe economic losses in both cucurbits and papaya throughout the tropics and subtropics. Development of PRSV-resistant transgenic plants faces a major hurdle in achieving resistance against geographically distinct isolates. One of the major reasons of failing to achieve the broad-...

journal_title：Molecular biotechnology

authors： Mangrauthia SK,Singh P,Praveen S

更新日期：2010-01-01 00:00:00

abstract：:Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...

journal_title：Molecular biotechnology

authors： Pegoraro M,Matić S,Pergolizzi B,Iannarelli L,Rossi AM,Morra M,Noris E

更新日期：2017-10-01 00:00:00

abstract：:Previous studies have shown that mRNA-electroporated dendritic cells (DCs) are able to process and present tumor-associated antigens, leading to the activation of tumor-specific T cells in vitro and in vivo. However, the optimal maturation state of antigen loading and half-life of the mRNA-translated protein product a...

journal_title：Molecular biotechnology

authors： Met O,Eriksen J,Svane IM

更新日期：2008-10-01 00:00:00

abstract：:Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials us...

journal_title：Molecular biotechnology

authors： Darling A

更新日期：2002-05-01 00:00:00

abstract：:The aim of this study was to evaluate the usefulness of replicating but non disseminating adenovirus vectors (AdVs) as vaccine vector using human rotavirus (HRV) as a model pathogen. HRV VP7, VP4, or VP4Δ (N-terminal 336 amino acids of VP4) structural proteins as well as the VP4Δ::VP7 chimeric fusion protein were expr...

journal_title：Molecular biotechnology

authors： Girard A,Roques E,St-Louis MC,Massie B,Archambault D

更新日期：2013-07-01 00:00:00

abstract：:Expression of certain transgenes from an adenovirus vector can be deleterious to its own replication. This can result in the inhibition of virus rescue, reduced viral yields, or, in the worst case, make it impossible to construct a vector expressing the inhibiting transgene product. A gene regulation system based on t...

journal_title：Molecular biotechnology

authors： Gall JG,Lizonova A,EttyReddy D,McVey D,Zuber M,Kovesdi I,Aughtman B,King CR,Brough DE

更新日期：2007-03-01 00:00:00

abstract：:Several mutations were introduced into the Cry1Ac toxin gene, resulting in four variants with altered sequences that were responsible for low expression of the toxin in transgenic plants. These variants were as follows: V1, with modified three A/T-rich regions, including the first signal of transcription termination; ...

journal_title：Molecular biotechnology

authors： Misztal LH,Mostowska A,Skibinska M,Bajsa J,Musial WG,Jarmolowski A

更新日期：2004-01-01 00:00:00

abstract：:Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...

journal_title：Molecular biotechnology

authors： Shamriz S,Ofoghi H

更新日期：2019-02-01 00:00:00

abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...

journal_title：Molecular biotechnology

authors： Rotman G,Vanagaite L,Collins FS,Shiloh Y

更新日期：1995-04-01 00:00:00

abstract：:Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...

journal_title：Molecular biotechnology

authors： Djurovic S,Iversen N,Jeansson S,Hoover F,Christensen G

更新日期：2004-09-01 00:00:00

abstract：:The biosensor field has grown enormously since the first demonstration of the biosensor concept by Leland C. Clark, Jr. in 1962. Today's biosensor market is dominated by glucose biosensors, mass-produced enzyme electrodes for the rapid self-diagnosis of blood glucose levels by diabetes sufferers. Here we take a histor...

journal_title：Molecular biotechnology

authors： Newman JD,Setford SJ

更新日期：2006-03-01 00:00:00

abstract：:A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vect...

journal_title：Molecular biotechnology

authors： Kang TJ,Kim BG,Yang JY,Yang MS

更新日期：2006-02-01 00:00:00

abstract：:Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...

journal_title：Molecular biotechnology

authors： Madonna R,Delli Pizzi S,Tartaro A,De Caterina R

更新日期：2014-05-01 00:00:00

abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...

journal_title：Molecular biotechnology

authors： do Nascimento NC,Menguer PK,Sperotto RA,de Almeida MR,Fett-Neto AG

更新日期：2013-05-01 00:00:00

abstract：:Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency of gRNA need to be developed. Three gRNAs with ...

journal_title：Molecular biotechnology

authors： Kim DH,Lee J,Suh Y,Lee K

更新日期：2021-02-01 00:00:00

abstract：:Adeno-associated virus (AAV) is a small, non-enveloped virus used as vector in gene therapy, mainly produced in human cells and in baculovirus systems. Intense studies on these platforms led to the production of vectors with titers between 103 and 105 viral genomes (vg) per cells. In spite of this, vector yields need ...

journal_title：Molecular biotechnology

authors： Galli A,Iaia I,Milella MS,Cipriani F,Della Latta V,Giacca M,Zentilin L,Cervelli T

更新日期：2021-02-01 00:00:00