解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency of gRNA need to be developed. Three gRNAs with ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00290-8
更新日期:2021-02-01 00:00:00
abstract::Adeno-associated virus (AAV) is a small, non-enveloped virus used as vector in gene therapy, mainly produced in human cells and in baculovirus systems. Intense studies on these platforms led to the production of vectors with titers between 103 and 105 viral genomes (vg) per cells. In spite of this, vector yields need ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00294-4
更新日期:2021-02-01 00:00:00
abstract::Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00281-9
更新日期:2021-01-01 00:00:00
abstract::DNA damage is one of the most impactful events in living organisms, leading to DNA sequence changes (mutation) and disruption of biological processes. A study has identified a protein called Damage Suppressor Protein (Dsup) in the tardigrade Ramazzotius varieornatus that has shown to reduce the effects of radiation da...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00273-9
更新日期:2020-12-01 00:00:00
abstract::D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00257-9
更新日期:2020-08-01 00:00:00
abstract::NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expans...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00250-2
更新日期:2020-05-01 00:00:00
abstract::Pasteurella multocida is the main cause of haemorrhagic septicaemia (HS) outbreak in livestock, such as cattle and buffaloes. Conventional vaccines such as alum-precipitated or oil-adjuvant broth bacterins were injected subcutaneously to provide protection against HS. However, the immunity developed is only for short ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00244-0
更新日期:2020-05-01 00:00:00
abstract::Classic toxicology studies often utilize in vivo animal models. Newer approaches employing in vitro organ-specific cellular models have been developed in recent years to help accelerate the speed and reduce the cost of traditional toxicology testing. Toward the goal of supporting in vitro cellular model research with ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00245-z
更新日期:2020-04-01 00:00:00
abstract::Plants synthesize amino acids by collateral metabolic pathways using primary elements carbon and oxygen from air, hydrogen from water in soil and nitrogen from soil. Following synthesis, amino acids are immediately used for metabolism, transient storage or transported to the phloem. Different families of transporters ...
journal_title:Molecular biotechnology
pub_type: 杂志文章,收录出版
doi:10.1007/s12033-020-00240-4
更新日期:2020-03-01 00:00:00
abstract::CD151 is an abundantly expressed eukaryotic transmembrane protein on the cell surface. It is involved in cell adhesion, angiogenesis and signal transduction as well in disease conditions such as cancer and viral infections. However, the molecular mechanism of CD151 activation is poorly understood due to the lack of st...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00212-3
更新日期:2019-12-01 00:00:00
abstract::In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00194-2
更新日期:2019-09-01 00:00:00
abstract::Lactic acid bacteria (LAB) are functional starter cultures in food and dairy industry and are also regarded as power houses for bioprocess and fermentation technology. Due to extensive applications in food and medical applications, intensive research and developmental activities are going on throughout the world to un...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-019-00186-2
更新日期:2019-08-01 00:00:00
abstract::Tall fescue plants are widely exposed to white grubs. Regarding the rate of damage caused by the white grubs to tall fescue and difficulty of its ecological and economical control, production of resistant cultivars is a priority. In this experiment, for the first time, we report production of transgenic lines resistan...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00183-5
更新日期:2019-07-01 00:00:00
abstract::D-Allulose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity. Using D-psicose epimerase and L-rhamnulose kinase, a method enabling high conversio...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00174-6
更新日期:2019-06-01 00:00:00
abstract::The REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) gene encodes a member of the ERF/AP2 transcription factor family involved in redox homeostasis. The RRTF1 gene shows tissue-specific responsiveness to various abiotic stress treatments including a response to salt stress in roots. An interesting feature of this respo...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00155-9
更新日期:2019-04-01 00:00:00
abstract::Ageing is a complex and progressive phenomenon, during which the accumulation of morphological and chemical changes seriously compromises the capacity of the cells to proliferate and fulfil their biological tasks. The increase in the average age of the world population, associated with a higher occurrence of age-relat...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00154-w
更新日期:2019-03-01 00:00:00
abstract::Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0140-1
更新日期:2019-02-01 00:00:00
abstract::Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achieveme...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-018-0133-0
更新日期:2019-01-01 00:00:00
abstract::D-Stereospecific amidohydrolase (DAH) from Streptomyces sp. 82F2 has potential utility for the synthesis of D/L configuration dipeptides by an aminolysis reaction. Structural comparison of DAH with substrate-bound D-amino acid amidase revealed that three residues located in the active site pocket of DAH (Thr145, Ala26...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0104-5
更新日期:2018-09-01 00:00:00
abstract::The GacS/A system in Azotobacter vinelandii regulates alginate and alkylresorcinols production through RsmZ1, a small regulatory RNA (sRNA) that releases the translational repression of the algD and arpR mRNAs caused by the RsmA protein. In the Pseudomonadaceae family, the Rsm-sRNAs are grouped into three families: Rs...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0102-7
更新日期:2018-09-01 00:00:00
abstract::In this study, we investigated expression and dimerization of an ER-associated degradation (ERAD) substrate, a null Hong Kong variant of α-1-antitrypsin (NHK) using immunoblotting assay and a novel NanoLuc complementary reporter system called the NanoBiT (NB) assay. This NB-tagged NHK made it possible to monitor the i...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0092-5
更新日期:2018-08-01 00:00:00
abstract::Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0094-3
更新日期:2018-08-01 00:00:00
abstract::Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant geno...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0075-6
更新日期:2018-06-01 00:00:00
abstract::The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0080-9
更新日期:2018-05-01 00:00:00
abstract::Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0065-8
更新日期:2018-03-01 00:00:00
abstract::The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0046-3
更新日期:2018-01-01 00:00:00
abstract::Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0030-y
更新日期:2017-10-01 00:00:00
abstract::Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0021-z
更新日期:2017-08-01 00:00:00
abstract::Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic analyses of the gut of C. curvignathus revealed...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0015-x
更新日期:2017-07-01 00:00:00
abstract::Derivation of cardiomyocytes directly from patients' own fibroblasts could offer a new therapeutic approach for those with ischemic heart disease. An essential step toward clinical application is to establish safe conversion of human fibroblasts into a cardiac fate. Here we aimed to efficiently and safely generate car...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0007-x
更新日期:2017-06-01 00:00:00
abstract::Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches h...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0003-1
更新日期:2017-05-01 00:00:00
abstract::Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study, a thermostable pectin methylesterase (CtPME) from Clostridium thermocellum belonging to family 8 carbohydrate...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-9997-7
更新日期:2017-05-01 00:00:00
abstract::Epoxide hydrolases (EHs; 3.3.2.x) catalyze the enantioselective ring opening of racemic epoxides to the corresponding enantiopure vicinal diols and remaining equivalent unreacted epoxides. These epoxides and diols are used for the synthesis of chiral drug intermediates. With an upsurge in the methods for identificatio...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-017-9996-8
更新日期:2017-03-01 00:00:00
abstract::Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated temperatures, including enhanced reaction rates, increased substrate solubility and decreased risks of contamination. Furthermore, thermophilic ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9975-5
更新日期:2016-11-01 00:00:00
abstract::The recombinant acidstable α-amylase (Ba-amy) of acidophilic bacterium Bacillus acidicola TSAS1 has been produced extracellularly using a combination of cloning (E. coli and P. pastoris) and physico-chemical treatment strategies. A total of 150,000 U/L of Ba-amy were attained under constitutive promoter in P. pastoris...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9970-x
更新日期:2016-11-01 00:00:00
abstract::PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9932-3
更新日期:2016-05-01 00:00:00
abstract::Transgenic rice seed can be utilized as a bioreactor to produce high-value recombinant proteins. Mouse interleukin 4 (mIL-4) and mIL-6 were specifically expressed as secretory proteins in rice endosperm by ligating the N-terminal glutelin B-1 (GluB-1) signal peptide and the C-terminal KDEL endoplasmic reticulum retent...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9920-7
更新日期:2016-04-01 00:00:00
abstract::Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9923-4
更新日期:2016-04-01 00:00:00
abstract::In this study cationic β-cyclodextrin-chitosan-mediated nanoparticles were used to transfer pmCherry-C1 into glioblastoma cells and their transfection efficiency were compared to lipofectamine and electroporation. Physicochemical characteristics of nanoparticles were evaluated by photon correlation spectroscopy and sc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9927-0
更新日期:2016-04-01 00:00:00
abstract::Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-015-9888-8
更新日期:2015-12-01 00:00:00