Abstract：
:Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials used in manufacture. Additional testing is also performed at various stages of production and, in some cases, on the final product as well. Because of inherent limitations in direct testing methods, the capacity of the downstream purification process to remove/inactivate potential viral contaminants is also studied to give an extra degree of assurance that the final product will be free of infectious viruses.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Darling Adoi
10.1385/MB:21:1:057keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
57-83issue
1eissn
1073-6085issn
1559-0305pii
MB:21:1:057journal_volume
21pub_type
杂志文章,评审abstract：:Menstrual blood has been recognized as an easily accessible and inexpensive source of stem cells, in recent years. To establish a safe and efficient protocol for development of menstrual blood-derived stem cells (MenSCs) into osteoblasts, the effect of substitution of fetal bovine serum (FBS) with human platelet deriv...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9698-9
更新日期：2014-03-01 00:00:00
abstract：:Brucellosis, one of the most important re-emerging zoonoses in many countries, is caused by bacteria belonging to the genus Brucella. Furthermore these bacteria represent potential biological warfare agents and the identification of species and biovars of field strains may be crucial for tracing back source of infecti...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9659-3
更新日期：2013-10-01 00:00:00
abstract：:Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher's disease, the most common inherited storage disorder. Treatment consists of enzyme replacement therapy by the administration of recombinant GCR produced in Chinese hamster ovary cells. The production of anti-GCR antibodies has already been...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9303-4
更新日期：2010-11-01 00:00:00
abstract：:We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9260-y
更新日期：2010-06-01 00:00:00
abstract：:Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase ge...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9094-z
更新日期：2009-01-01 00:00:00
abstract：:The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation, playing important functions in various biological processes. The C-terminus of the β7 (PSMB4) proteasome subunit was tagged with EGFP and with a composite element for affinity purification and TEV cl...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9799-0
更新日期：2015-01-01 00:00:00
abstract：:The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia A...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9423-5
更新日期：2012-03-01 00:00:00
abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9066-3
更新日期：2008-09-01 00:00:00
abstract：:Neuroblastoma is the most common extracranial solid tumor of infancy. Amplification of MYCN oncogene is found in approximately 20 % of all neuroblastoma patients and correlates with advanced disease stages, rapid tumor progression, and poor prognosis, making this gene an obvious therapeutic target. However, being a tr...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9739-z
更新日期：2014-07-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0094-3
更新日期：2018-08-01 00:00:00
abstract：:Gene reporter systems play a key role in gene expression and regulation studies. This review describes the ideal reporter systems, including reporter expression vector design. It summarizes the many uses of genetic reporters and outlines the currently available and commonly used reporter systems. Each system is descri...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:13:1:29
更新日期：1999-11-01 00:00:00
abstract：:In an effort to develop microalgae as a robust system for the production of valuable proteins, we analyzed some of the factors affecting recombinant protein expression in the chloroplast of the green alga Chlamydomonas reinhardtii. We monitored mRNA accumulation, protein synthesis, and protein turnover for three codon...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9348-4
更新日期：2011-05-01 00:00:00
abstract：:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:14:1:01
更新日期：2000-01-01 00:00:00
abstract：:Characterization of novel plant gene promoters underpins the development of transgenic crop plants. Here, we report a novel 5' regulatory sequence (Eriubi D7) of the ubiquitin gene from Erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. A 3.2-kb regulatory sequence of u...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9875-0
更新日期：2015-09-01 00:00:00
abstract：:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-019-00194-2
更新日期：2019-09-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9135-7
更新日期：2009-05-01 00:00:00
abstract：:Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:28:1:21
更新日期：2004-09-01 00:00:00
abstract：:A bacterial artificial chromosome library of the causal agent of the Black Sigatoka leaf spot disease of banana and plantain, Mycosphaerella fijiensis, has been constructed using a non-sphaeroplasting technique and characterized using both homologous and heterologous probes. After first and a second size selection of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-0016-2
更新日期：2007-05-01 00:00:00
abstract：:The Embrapa 5.1 genetically modified (GM) common bean was approved for commercialization in Brazil. Methods for the quantification of this new genetically modified organism (GMO) are necessary. The development of a suitable endogenous reference is essential for GMO quantification by real-time PCR. Based on this, a new...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9786-5
更新日期：2014-11-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02752254
更新日期：1997-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9500-4
更新日期：2013-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:31:2:103
更新日期：2005-10-01 00:00:00
abstract：:The implementation of efficient technologies for the production of recombinant mammalian membrane receptors is an outstanding challenge in understanding receptor-ligand actions and the development of therapeutic antibodies. In order to improve the solubility of recombinant extracellular domains of human membrane recep...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:33:3:199
更新日期：2006-07-01 00:00:00
abstract：:We investigated the efficiency of a single plasmid transfection along the longitudinal axis of the regenerating soleus of young rats. This also reflected transfection efficiency along the fibers because the soleus is a nearly fusiform muscle in young animals. The complete regeneration was induced by notexin and the tr...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9555-2
更新日期：2013-06-01 00:00:00
abstract：:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02761713
更新日期：1996-12-01 00:00:00
abstract：:The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity. In this protocol, we describe the preparation of plasmid and PCR-derived templates. A basic transcription protocol is provided with several optional modifications. The use of RNA probes in Northern blot...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02761751
更新日期：1997-04-01 00:00:00
abstract：:Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02740841
更新日期：1998-12-01 00:00:00
abstract：:Human adipose tissue-derived stromal cells (ADSCs) are being evaluated for cardiovascular repair. We developed an ex vivo method for producing angiogenic ADSCs transduced with a self-inactivating lentiviral vector (LV) expressing the enhanced green fluorescence protein (EGFP) from an internal cytomegalovirus (CMV) pro...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9537-4
更新日期：2013-05-01 00:00:00
abstract：:Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic analyses of the gut of C. curvignathus revealed...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0015-x
更新日期：2017-07-01 00:00:00
abstract：:Alternaria blight caused by Alternaria brassicicola (Schwein.) Wiltshire and A. brassicae (Berk.) Sacc., is one of the most important disease of rapeseed-mustard, characterized by the formation of spots on leaves, stem, and siliquae with premature defoliation and stunting of growth. These two species are very difficul...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9784-7
更新日期：2014-11-01 00:00:00
