Abstract：
:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, were selected and used as primers in PCR and RT-PCR. We first applied the restriction primers to the plasmid DNA and bacterial (Pseudomonas) and plant (Cymbidium) genomic DNAs. We observed positive DNA amplifications with the recombinant plasmid DNA and bacterial and plant genomic DNAs. Purified viral RNA was used for template in the RT-PCR with the primers and successful DNA amplification was obtained. These results suggest that the 6-mer restriction primers can be useful for new applications in PCR.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Ryu KH,Choi SH,Lee JSdoi
10.1385/MB:14:1:01keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
1-3issue
1eissn
1073-6085issn
1559-0305pii
MB:14:1:01journal_volume
14pub_type
杂志文章abstract：:PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9932-3
更新日期：2016-05-01 00:00:00
abstract：:Modern sugarcane cultivars have complex genetic characteristics and low fertility that render their genetic improvement through traditional breeding difficult. Genetic engineering methodology to introduce foreign genes provides new opportunities for the genetic improvement of sugarcane cultivars. One of prerequisites ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:28:2:113
更新日期：2004-10-01 00:00:00
abstract：:Tea, a beverage crop, is a rich source of polyphenols and polysaccharides which greatly attribute to its importance. However, oxidation and precipitation of these compounds during nucleic acids extraction is a limitation to molecular biology and genomic studies. On isolation of total RNA from root tissue using establi...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9476-5
更新日期：2012-09-01 00:00:00
abstract：:The GacS/A system in Azotobacter vinelandii regulates alginate and alkylresorcinols production through RsmZ1, a small regulatory RNA (sRNA) that releases the translational repression of the algD and arpR mRNAs caused by the RsmA protein. In the Pseudomonadaceae family, the Rsm-sRNAs are grouped into three families: Rs...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0102-7
更新日期：2018-09-01 00:00:00
abstract：:Expression of certain transgenes from an adenovirus vector can be deleterious to its own replication. This can result in the inhibition of virus rescue, reduced viral yields, or, in the worst case, make it impossible to construct a vector expressing the inhibiting transgene product. A gene regulation system based on t...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02686012
更新日期：2007-03-01 00:00:00
abstract：:A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vect...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:32:2:093
更新日期：2006-02-01 00:00:00
abstract：:Host protein synthesis is shut down in the lytic baculovirus expression vector system (BEVS). This also affects host proteins involved in routing secretory proteins through the endoplasmic reticulum (ER)-Golgi system. It has been demonstrated that a secretory alkaline phosphatase-EGFP fusion protein (SEFP) can act as ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9545-4
更新日期：2013-05-01 00:00:00
abstract：:To develop combined vaccine systems, we have generated Edwardsiella tarda ghosts (ETG) displaying a foreign protein on the outer membrane and also Ed. tarda cadaver (ETC) expressing a heterologous protein in the cytoplasm. Green fluorescent protein (GFP) was used as a model foreign protein. A constitutive promoter (Et...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9277-2
更新日期：2010-10-01 00:00:00
abstract：:The REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) gene encodes a member of the ERF/AP2 transcription factor family involved in redox homeostasis. The RRTF1 gene shows tissue-specific responsiveness to various abiotic stress treatments including a response to salt stress in roots. An interesting feature of this respo...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-019-00155-9
更新日期：2019-04-01 00:00:00
abstract：:Indirubin is a strong inhibitor of several eukaryotic cell signaling pathways and shows promise as a treatment for myelocytic leukemia and Alzheimer's disease. The tmoABCDEF operon, encoding the components of a novel toluene 4-monooxygenase from the paint factory soil isolate, Pseudomonas sp. M4, was cloned and expres...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9863-4
更新日期：2015-08-01 00:00:00
abstract：:Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789331
更新日期：1995-06-01 00:00:00
abstract：:IMGT, the International ImMunoGeneTics information system ( http://imgt.cines.fr ), was created in 1989 by the Laboratoire d'ImmunoGénétique Moléculaire (LIGM) (Université Montpellier 2 and CNRS) at Montpellier, France, in order to standardize and manage the complexity of immunogenetics data. IMGT is recognized as the...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9062-7
更新日期：2008-09-01 00:00:00
abstract：:Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches h...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0003-1
更新日期：2017-05-01 00:00:00
abstract：:Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadv...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:17:3:219
更新日期：2001-03-01 00:00:00
abstract：:Immobilized-metal-ion affinity chromatography (IMAC) is used extensively for phosphopeptide enrichment in phosphoproteomics. However, the effect of nucleic acids in protein samples on phosphopeptide enrichment by IMAC has not yet been well clarified. In this study, we demonstrate that IMAC beads possess a strong adsor...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9176-6
更新日期：2009-09-01 00:00:00
abstract：:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02761713
更新日期：1996-12-01 00:00:00
abstract：:Neuroblastoma is the most common extracranial solid tumor of infancy. Amplification of MYCN oncogene is found in approximately 20 % of all neuroblastoma patients and correlates with advanced disease stages, rapid tumor progression, and poor prognosis, making this gene an obvious therapeutic target. However, being a tr...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9739-z
更新日期：2014-07-01 00:00:00
abstract：:The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02900362
更新日期：1996-06-01 00:00:00
abstract：:High-quality RNA preparations are critical for further applications such as reverse transcriptase-polymerase chain reaction (RT-PCR) transcript amplifications, and elaboration of cDNA and expressed sequence tag libraries. Melanins are phenolic compounds present in many fungi and apparently play key roles in fungi path...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:34:1:45
更新日期：2006-09-01 00:00:00
abstract：:RNA editing is one of the post-transcriptional RNA processes. RNA editing generates RNA and protein diversity in eukaryotes and results in specific amino acid substitutions, deletions, and changes in gene expression levels. Adenosine-to-inosine RNA editing represents the most important class of editing in human and af...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-012-9498-7
更新日期：2012-09-01 00:00:00
abstract：:As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:14:3:197
更新日期：2000-03-01 00:00:00
abstract：:Lactic acid bacteria (LAB) are generally sensitive to hydrogen peroxide (H(2)O(2)), Lactobacillus sakei YSI8 is one of the very few LAB strains able to degrade H(2)O(2) through the action of a heme-dependent catalase. Lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product ferment...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9254-9
更新日期：2010-06-01 00:00:00
abstract：:Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0030-y
更新日期：2017-10-01 00:00:00
abstract：:A shuttle vector pZL1 which can replicate both in Gluconobacter oxydans and Escherichia coli was constructed based on G. oxydans DSM2003 cryptic plasmid pGOX3, a homology of G. oxydans 621H pGOX3, and E. coli cloning vector pUC18. It was found to be stably maintained in G. oxydans during the serial subcultures in the ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9293-2
更新日期：2010-11-01 00:00:00
abstract：:NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expans...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00250-2
更新日期：2020-05-01 00:00:00
abstract：:Menstrual blood has been recognized as an easily accessible and inexpensive source of stem cells, in recent years. To establish a safe and efficient protocol for development of menstrual blood-derived stem cells (MenSCs) into osteoblasts, the effect of substitution of fetal bovine serum (FBS) with human platelet deriv...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9698-9
更新日期：2014-03-01 00:00:00
abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:17:2:119
更新日期：2001-02-01 00:00:00
abstract：:The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0046-3
更新日期：2018-01-01 00:00:00
abstract：:The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0080-9
更新日期：2018-05-01 00:00:00
abstract：:De novo design provides an in silico toolkit for the design of novel small molecular structures to a set of specified structural constraints. With the avalanche of bioinformatics data, de novo design is ideally suited for exploring molecules that could be useful for chemical genomics. The design process involves manip...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-007-0037-x
更新日期：2007-11-01 00:00:00
