Coincidence cloning. Taking the coincidences out of genome analysis.

abstract：:Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...

journal_title：Molecular biotechnology

authors： Díaz-Dinamarca DA,Jerias JI,Soto DA,Soto JA,Díaz NV,Leyton YY,Villegas RA,Kalergis AM,Vásquez AE

更新日期：2018-03-01 00:00:00

abstract：:The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach...

journal_title：Molecular biotechnology

authors： Lou H,Ye Z,Yun F,Lin J,Guo L,Chen B,Mu Z

更新日期：2018-05-01 00:00:00

abstract：:The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...

journal_title：Molecular biotechnology

authors： Long C,Cheng Y,Cui J,Liu J,Gan L,Zeng B,Long M

更新日期：2018-01-01 00:00:00

abstract：:Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor, that has been used as a therapeutic agent in facilitating bone marrow and stem cell transplantation and in other clinical cases like neutropenia. Although biologically active recombinant GM-CSF has been successfully produced in...

journal_title：Molecular biotechnology

authors： Bhattacharya P,Pandey G,Srivastava P,Mukherjee KJ

更新日期：2005-06-01 00:00:00

abstract：:Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...

journal_title：Molecular biotechnology

authors： Shamriz S,Ofoghi H

更新日期：2019-02-01 00:00:00

abstract：:A sensitive method for staining proteins after transfer from polyacrylamide gels to nitrocellulose paper is described. Transferred proteins are first derivatized by reaction of the nitrocellulose replica with sulfosuccinimidobiotin and are then reacted sequentially with streptavidin, rabbit anti-streptavidin, and hors...

journal_title：Molecular biotechnology

authors： Santora KE,Nelson SA,Lewis KA,LaRochelle WJ

更新日期：2000-06-01 00:00:00

abstract：:Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant geno...

journal_title：Molecular biotechnology

authors： Zvirin T,Magrisso L,Yaari A,Shoseyov O

更新日期：2018-06-01 00:00:00

abstract：:A sandwich-type enzyme-linked immunosorbent assay (ELISA) was established for monitoring the secretion of ZZ-fusion proteins. Two antibodies, a monoclonal mouse anti-human proinsulin and a rabbit anti-bovine IgG (strongly binding to the ZZ-domain), were used to quantify the secretion of recombinant human ZZ-proinsulin...

journal_title：Molecular biotechnology

authors： Mergulhão FJ,Monteiro GA,Cabral JM,Taipa MA

更新日期：2001-11-01 00:00:00

abstract：:We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...

journal_title：Molecular biotechnology

authors： Todorov T,Todorova A,Georgieva B,Mitev V

更新日期：2010-06-01 00:00:00

abstract：:The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utilized this property o...

journal_title：Molecular biotechnology

authors： Fuchs B,Zhang K,Rock MG,Bolander ME,Sarkar G

更新日期：1999-10-01 00:00:00

abstract：:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...

journal_title：Molecular biotechnology

authors： Vijayalakshmi MA

更新日期：1996-12-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field ...

journal_title：Molecular biotechnology

authors： Jurinke C,Oeth P,van den Boom D

更新日期：2004-02-01 00:00:00

abstract：:The diversity and complexity of bioinformatics tools currently available for protein sequence analysis can make it difficult to know where to begin when presented with a new sequence. In this article, we present a protocol outlining one approach to sequence analysis that should give as comprehensive a picture as possi...

journal_title：Molecular biotechnology

authors： Russell RB

更新日期：2007-07-01 00:00:00

abstract：:Despite the known importance of the composition of culture media and culture conditions on Bacillus thuringiensis growth and toxicity, very few reviews are concerned with this subject. This article reviews some aspects of the microbiology of Bacillus thuringiensis, and how toxicity is affected by the composition of gr...

journal_title：Molecular biotechnology

authors： Avignone-Rossa C,Mignone CF

更新日期：1995-08-01 00:00:00

abstract：:The ability to identify disease-associated genes using microarray technology is dependent on isolating high-quality total RNA from the diseased tissue under study. However, it is not always possible to obtain large amounts of affected tissue from patients and therefore the quality and yield of RNA may be compromised. ...

journal_title：Molecular biotechnology

authors： Murray EJ

更新日期：2003-02-01 00:00:00

abstract：:In this study, we investigated expression and dimerization of an ER-associated degradation (ERAD) substrate, a null Hong Kong variant of α-1-antitrypsin (NHK) using immunoblotting assay and a novel NanoLuc complementary reporter system called the NanoBiT (NB) assay. This NB-tagged NHK made it possible to monitor the i...

journal_title：Molecular biotechnology

authors： Norisada J,Fujimura K,Amaya F,Kohno H,Hirata Y,Oh-Hashi K

更新日期：2018-08-01 00:00:00

abstract：:L-Cysteine desulfhydrase (DES; EC 4.4.1.1) is the most important enzyme that catalyzes the decomposition of L-cysteine to pyruvate, ammonia, and hydrogen sulfide (H2S), the latter of which has recently been recognized as the third gasotransmitter for multiple signaling events in plants. Previous results showed the exi...

journal_title：Molecular biotechnology

authors： Xie Y,Lai D,Mao Y,Zhang W,Shen W,Guan R

更新日期：2013-07-01 00:00:00

abstract：:Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study, a thermostable pectin methylesterase (CtPME) from Clostridium thermocellum belonging to family 8 carbohydrate...

journal_title：Molecular biotechnology

authors： Rajulapati V,Goyal A

更新日期：2017-05-01 00:00:00

abstract：:An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could repr...

journal_title：Molecular biotechnology

authors： Wang Z,Wang HY,Feng H

更新日期：2013-01-01 00:00:00

abstract：:HOX genes are highly conserved transcription factors that determine the identity of cells and tissues along the anterior-posterior body axis in developing embryos. Aberrations in HOX gene expression have been shown in various tumors. However, the correlation of HOX gene expression patterns with tumorigenesis and cance...

journal_title：Molecular biotechnology

authors： Hur H,Lee JY,Yun HJ,Park BW,Kim MH

更新日期：2014-01-01 00:00:00

abstract：:Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches h...

journal_title：Molecular biotechnology

authors： Morokuma D,Xu J,Hino M,Mon H,Merzaban JS,Takahashi M,Kusakabe T,Lee JM

更新日期：2017-05-01 00:00:00

abstract：:Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials us...

journal_title：Molecular biotechnology

authors： Darling A

更新日期：2002-05-01 00:00:00

abstract：:Indirubin is a strong inhibitor of several eukaryotic cell signaling pathways and shows promise as a treatment for myelocytic leukemia and Alzheimer's disease. The tmoABCDEF operon, encoding the components of a novel toluene 4-monooxygenase from the paint factory soil isolate, Pseudomonas sp. M4, was cloned and expres...

journal_title：Molecular biotechnology

authors： Wongsaroj L,Sallabhan R,Dubbs JM,Mongkolsuk S,Loprasert S

更新日期：2015-08-01 00:00:00

abstract：:To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most pro...

journal_title：Molecular biotechnology

authors： Gach JS,Quendler H,Ferko B,Katinger H,Kunert R

更新日期：2008-06-01 00:00:00

abstract：:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...

journal_title：Molecular biotechnology

authors： Bekli S,Aktas B,Gencer D,Aslim B

更新日期：2019-09-01 00:00:00

abstract：:Human immunodeficiency virus types 1 and 2 (HIV-1, -2), the etiological agents of AIDS, are retroviruses that replicate in CD4+ T lymphocytes, monocytes, and macrophages. Early anti-HIV therapies were directed at the step in the virus life cycle that was considered the most readily blocked: the transcription of viral ...

journal_title：Molecular biotechnology

authors： Patience C,Moore J,Boyd M

更新日期：1994-02-01 00:00:00

abstract：:Genes are recognized as undergoing genomic imprinting when they are capable of being expressed only from the paternal or only from the maternal chromosome. The process can occur coordinately within large physical domains in mammalian chromosomes. One interesting facet of the study of genomic imprinting is that it offe...

journal_title：Molecular biotechnology

authors： Greally JM

更新日期：1999-04-01 00:00:00

abstract：:Immobilized-metal-ion affinity chromatography (IMAC) is used extensively for phosphopeptide enrichment in phosphoproteomics. However, the effect of nucleic acids in protein samples on phosphopeptide enrichment by IMAC has not yet been well clarified. In this study, we demonstrate that IMAC beads possess a strong adsor...

journal_title：Molecular biotechnology

authors： Li Y,Luo Y,Wu S,Gao Y,Liu Y,Zheng D

更新日期：2009-09-01 00:00:00

abstract：:PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...

journal_title：Molecular biotechnology

authors： Lorenzo V,Mascanzoni F,Vitagliano L,Ruvo M,Doti N

更新日期：2016-05-01 00:00:00

abstract：:Tea, a beverage crop, is a rich source of polyphenols and polysaccharides which greatly attribute to its importance. However, oxidation and precipitation of these compounds during nucleic acids extraction is a limitation to molecular biology and genomic studies. On isolation of total RNA from root tissue using establi...

journal_title：Molecular biotechnology

authors： Muoki RC,Paul A,Kumari A,Singh K,Kumar S

更新日期：2012-09-01 00:00:00