Genomic imprinting and chromatin insulation in Beckwith-Wiedemann syndrome.

abstract：:Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...

journal_title：Molecular biotechnology

authors： Naik V,Kardani J,Roy I

更新日期：2016-04-01 00:00:00

abstract：:Tumor necrosis factor (TNF) is a central cytokine in the pathogenesis of septic shock and other inflammatory states. Assay by immunoassay is convenient, but, because of circulating soluble receptors, does not accurately reflect biological activity of the cytokine. This article describes how to perform a bioassay for T...

journal_title：Molecular biotechnology

authors： Evans TJ

更新日期：2000-07-01 00:00:00

abstract：:Pasteurella multocida is the main cause of haemorrhagic septicaemia (HS) outbreak in livestock, such as cattle and buffaloes. Conventional vaccines such as alum-precipitated or oil-adjuvant broth bacterins were injected subcutaneously to provide protection against HS. However, the immunity developed is only for short ...

journal_title：Molecular biotechnology

authors： Chelliah S,Velappan RD,Lim KT,Swee CWK,Nor Rashid N,Rothan HA,Kabir N,Ismail S

更新日期：2020-05-01 00:00:00

abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...

journal_title：Molecular biotechnology

authors： Yang Y,Wang Q,Guo D

更新日期：2008-09-01 00:00:00

abstract：:In this study, human alpha-1,4-N-acetylglucosaminyltransferase (alpha4GnT) fused with GFP(uv) (GFP(uv)-alpha4GnT) was expressed using both a transformed cell system and silkworm larvae. A Tn-pXgp-GFP(uv)-alpha4GnT cell line, isolated after expression vector transfection, produced 106 mU/ml of alpha4GnT activity in sus...

journal_title：Molecular biotechnology

authors： Nakajima M,Kato T,Kanamasa S,Park EY

更新日期：2009-09-01 00:00:00

abstract：:A total of 41 accessions of Aglianico belonging to three different biotypes (Taburno, Taurasi, and Vulture) and 9 accessions of Sirica grapes were sampled from diverse areas of Campania (Italy). All accessions were first genotyped using 21 microsatellite markers (SSR) to evaluate possible homonymies, synonymies, and t...

journal_title：Molecular biotechnology

authors： De Lorenzis G,Imazio S,Biagini B,Failla O,Scienza A

更新日期：2013-06-01 00:00:00

abstract：:The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticu...

journal_title：Molecular biotechnology

authors： Cai H,Tian S,Dong H

更新日期：2012-10-01 00:00:00

abstract：:Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but ...

journal_title：Molecular biotechnology

authors： Yuan YJ,Ma ZY,Wu JC

更新日期：2002-02-01 00:00:00

abstract：:In the context of clinical gene transfer using viral vectors, the risk of memory antivector immunity is often poorly appreciated. The immunological past of the patient, the site of injection, and the vector dose will play intertwined and decisive roles in the safety and efficacy of treatment. To circumvent the drawbac...

journal_title：Molecular biotechnology

authors： Perreau M,Kremer EJ

更新日期：2006-10-01 00:00:00

abstract：:Sample preparation is crucial for obtaining high-quality proteins for the purpose of electrophoretic separation and further analysis from tissues that contain high levels of interfering compounds. Hypericum perforatum is a medicinal plant that contains high amounts of phenolic compounds, of which hypericins, hyperfori...

journal_title：Molecular biotechnology

authors： Karppinen K,Taulavuori E,Hohtola A

更新日期：2010-11-01 00:00:00

abstract：:Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases ...

journal_title：Molecular biotechnology

authors： Dimopoulou M,Claisse O,Dutilh L,Miot-Sertier C,Ballestra P,Lucas PM,Dols-Lafargue M

更新日期：2017-08-01 00:00:00

abstract：:Brn-3a, a member of the POU gene family (so-called because of the similarity with the group of transcription factors Pit, Oct, and Unc), was found in neuronal cells engaged in the transcription activity of the p1 and p2 promoters of the most powerful antiapoptotic gene, namely, Bcl-2. The alternative splicing of Brn-3...

journal_title：Molecular biotechnology

authors： Chiarugi V,Del Rosso M,Magnelli L

更新日期：2002-10-01 00:00:00

abstract：:Lactococcus lactis is industrially important microorganism used in many dairy fermentations. Numerous genes and gene expression signals from this organism have now been identified and characterized. Recently, several naturally occurring, inducible gene-expression systems have also been described in L. lactis. The main...

journal_title：Molecular biotechnology

authors： Djordjevic GM,Klaenhammer TR

更新日期：1998-04-01 00:00:00

abstract：:IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalyti...

journal_title：Molecular biotechnology

authors： Mitsuda Y,Planque S,Hara M,Kyle R,Taguchi H,Nishiyama Y,Paul S

更新日期：2007-06-01 00:00:00

abstract：:Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technol...

journal_title：Molecular biotechnology

authors： McCarthy JJ

更新日期：2003-11-01 00:00:00

abstract：:The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach...

journal_title：Molecular biotechnology

authors： Lou H,Ye Z,Yun F,Lin J,Guo L,Chen B,Mu Z

更新日期：2018-05-01 00:00:00

abstract：:Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...

journal_title：Molecular biotechnology

authors： Arneaud SL,Porter JR

更新日期：2015-12-01 00:00:00

abstract：:Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last y...

journal_title：Molecular biotechnology

authors： Thiele A,Stangl GI,Schutkowski M

更新日期：2011-11-01 00:00:00

abstract：:Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadv...

journal_title：Molecular biotechnology

authors： Christensen AC

更新日期：2001-03-01 00:00:00

abstract：:Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency of gRNA need to be developed. Three gRNAs with ...

journal_title：Molecular biotechnology

authors： Kim DH,Lee J,Suh Y,Lee K

更新日期：2021-02-01 00:00:00

abstract：:The gram-negative anaerobic oral bacterium Porphyromonas gingivalis initiates periodontal disease through fimbrial attachment to saliva-coated oral surfaces. To study the effects of immunomodulation on enhancement of subunit vaccination, the expression in E. coli and immunogenicity of P. gingivalis fimbrial protein (F...

journal_title：Molecular biotechnology

authors： Kim TG,Huy NX,Kim MY,Jeong DK,Jang YS,Yang MS,Langridge WH,Lee JY

更新日期：2009-02-01 00:00:00

abstract：:Recombinant proteins are useful tools in biological research, drug development, and drug screening. Specially designed expression vectors have been developed to introduce cDNA for recombinant protein expression in mammalian cells. We have combined a dicistronic mRNA design for expression of the recombinant protein, us...

journal_title：Molecular biotechnology

authors： Pu H,Cashion LM,Kretschmer PJ,Liu Z

更新日期：1998-08-01 00:00:00

abstract：:The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organ...

journal_title：Molecular biotechnology

authors： Gatter M,Gatter T,Matthäus F

更新日期：2012-10-01 00:00:00

abstract：:Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow ...

journal_title：Molecular biotechnology

authors： Graham FL,Prevec L

更新日期：1995-06-01 00:00:00

abstract：:A sensitive method for staining proteins after transfer from polyacrylamide gels to nitrocellulose paper is described. Transferred proteins are first derivatized by reaction of the nitrocellulose replica with sulfosuccinimidobiotin and are then reacted sequentially with streptavidin, rabbit anti-streptavidin, and hors...

journal_title：Molecular biotechnology

authors： Santora KE,Nelson SA,Lewis KA,LaRochelle WJ

更新日期：2000-06-01 00:00:00

abstract：:Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...

journal_title：Molecular biotechnology

authors： Noble JE,Knight AE,Reason AJ,Di Matola A,Bailey MJ

更新日期：2007-10-01 00:00:00

abstract：:The simple use of nonisotopic hybridization probes to detect complementary sequences provides valuable information in a large number of research and commercial applications. In hybridization assays, the four "S's (speed, simplicity, sensitivity, and specificity) are important criteria for determining the choice of pro...

journal_title：Molecular biotechnology

authors： Ruth JL

更新日期：1996-10-01 00:00:00

abstract：:The REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) gene encodes a member of the ERF/AP2 transcription factor family involved in redox homeostasis. The RRTF1 gene shows tissue-specific responsiveness to various abiotic stress treatments including a response to salt stress in roots. An interesting feature of this respo...

journal_title：Molecular biotechnology

authors： Soliman ERS,Meyer P

更新日期：2019-04-01 00:00:00

abstract：:A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...

journal_title：Molecular biotechnology

authors： Wang TT,Cheng WC,Lee BH

更新日期：1998-10-01 00:00:00

abstract：:Heat shock proteins (HSPs) are upregulated in response to stress and play a protective function in refolding of cellular proteins. In hypertension, the heart is a vital organ that requires examination and investigation, and primary induction of HSPs is predominantly effected. Hypertension results from osmotic imbalanc...

journal_title：Molecular biotechnology

authors： Mala JG,Takeuchi S

更新日期：2009-06-01 00:00:00