Abstract：
:IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalytic potential of the antibody classes with minimal effects of noncovalent interactions occurring at sites remote from the reaction center. The IgA preparations hydrolyzed Glu-Ala-Arg-AMC at rates 3-orders of magnitude greater than IgG preparations from the same individuals. The cleavage occurred preferentially on the C terminal side of a basic residue. The activity was confirmed using monoclonal IgAs isolated from patients with multiple myeloma. Active site-directed inhibitors of serine proteases inhibited the catalytic activity and were bound irreversibly by the IgA, suggesting the involvement of a serine protease-like mechanism similar to that utilized by previously described IgM antibodies. These observations suggest that mechanisms underlying B cell clonal selection favor the retention and improvement of catalytic activity in the IgA, but not the IgG compartment of the immune response.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Mitsuda Y,Planque S,Hara M,Kyle R,Taguchi H,Nishiyama Y,Paul Sdoi
10.1007/s12033-007-0003-7subject
Has Abstractpub_date
2007-06-01 00:00:00pages
113-22issue
2eissn
1073-6085issn
1559-0305pii
MB:36:2:113journal_volume
36pub_type
杂志文章abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...
journal_title：Molecular biotechnology
pub_type： 杂志文章
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更新日期：2001-02-01 00:00:00
abstract：:This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be den...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02779017
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abstract：:Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02740841
更新日期：1998-12-01 00:00:00
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journal_title：Molecular biotechnology
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doi：10.1007/s12033-020-00290-8
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:31:1:001
更新日期：2005-09-01 00:00:00
abstract：:Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789331
更新日期：1995-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9516-9
更新日期：2013-01-01 00:00:00
abstract：:Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...
journal_title：Molecular biotechnology
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更新日期：2018-03-01 00:00:00
abstract：:Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...
journal_title：Molecular biotechnology
pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
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journal_title：Molecular biotechnology
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更新日期：2006-11-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9231-3
更新日期：2010-03-01 00:00:00
abstract：:Considering the fact that Prays oleae is one of the most pathogenic insects to the olive tree in the Mediterranean basin, particularly in Tunisia, the mode of action of Cry insecticidal toxins of Bacillus thuringiensis kurstaki in Prays oleae midgut was investigated. The proteolysis of Bacillus thuringiensis delta-end...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02686109
更新日期：2007-02-01 00:00:00
abstract：:The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia A...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9423-5
更新日期：2012-03-01 00:00:00
abstract：:We describe an expression system for high-yield production of recombinant soluble human FasL (rsh- FasL) in CHO cells. After one round of selection for gene amplification, cell lines producing rsh-FasL up to 60 microg/L x 10(6) cells in 24 h were obtained. Cell lines were grown in protein-free medium as suspension cul...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:23:3:189
更新日期：2003-03-01 00:00:00
abstract：:Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase ge...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9094-z
更新日期：2009-01-01 00:00:00
abstract：:Immobilized-metal-ion affinity chromatography (IMAC) is used extensively for phosphopeptide enrichment in phosphoproteomics. However, the effect of nucleic acids in protein samples on phosphopeptide enrichment by IMAC has not yet been well clarified. In this study, we demonstrate that IMAC beads possess a strong adsor...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9176-6
更新日期：2009-09-01 00:00:00
abstract：:ATP-binding cassette transporter G2 (ABCG2) gene encodes a protein that has a wide variety of substrates and is responsible for the active secretion of clinically and toxicologically important molecules into milk. Although known in many species, this marks the first time this gene product has been reported in goats. I...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-9024-5
更新日期：2008-05-01 00:00:00
abstract：:In this study, we investigated expression and dimerization of an ER-associated degradation (ERAD) substrate, a null Hong Kong variant of α-1-antitrypsin (NHK) using immunoblotting assay and a novel NanoLuc complementary reporter system called the NanoBiT (NB) assay. This NB-tagged NHK made it possible to monitor the i...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0092-5
更新日期：2018-08-01 00:00:00
abstract：:An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained b...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02752699
更新日期：1998-02-01 00:00:00
abstract：:Menstrual blood has been recognized as an easily accessible and inexpensive source of stem cells, in recent years. To establish a safe and efficient protocol for development of menstrual blood-derived stem cells (MenSCs) into osteoblasts, the effect of substitution of fetal bovine serum (FBS) with human platelet deriv...
journal_title：Molecular biotechnology
pub_type： 杂志文章
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更新日期：2014-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12033-007-0032-2
更新日期：2007-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-04-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9545-4
更新日期：2013-05-01 00:00:00
abstract：:Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9888-8
更新日期：2015-12-01 00:00:00
abstract：:A short (43-bp) A/T-rich stretch of DNA located in the intergenic region between the baiA2 and baiF genes from Eubacterium sp. strain VPI 12708 was amplified by polymerase chain reaction (PCR) and inserted in front of the Shine-Dalgarno (SD) sequences of three inefficiently-expressed Eubacterium sp. strain VPI 12708 g...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789174
更新日期：1999-02-01 00:00:00
abstract：:Lentiviral transduction is a highly efficient DNA delivery method for RNA interference applications. However, obtaining high lentiviral titers of shRNA and miRNA encoding vectors is challenging, since shRNA and miRNA cassettes have been shown to reduce lentiviral titers. In this study, we compare four commercially ava...
journal_title：Molecular biotechnology
pub_type： 杂志文章
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更新日期：2015-06-01 00:00:00
abstract：:The simple use of nonisotopic hybridization probes to detect complementary sequences provides valuable information in a large number of research and commercial applications. In hybridization assays, the four "S's (speed, simplicity, sensitivity, and specificity) are important criteria for determining the choice of pro...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02740771
更新日期：1996-10-01 00:00:00
abstract：:Characterization of novel plant gene promoters underpins the development of transgenic crop plants. Here, we report a novel 5' regulatory sequence (Eriubi D7) of the ubiquitin gene from Erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. A 3.2-kb regulatory sequence of u...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9875-0
更新日期：2015-09-01 00:00:00
abstract：:In the context of clinical gene transfer using viral vectors, the risk of memory antivector immunity is often poorly appreciated. The immunological past of the patient, the site of injection, and the vector dose will play intertwined and decisive roles in the safety and efficacy of treatment. To circumvent the drawbac...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:34:2:247
更新日期：2006-10-01 00:00:00
abstract：:The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02761713
更新日期：1996-12-01 00:00:00
