Comparative effect of human platelet derivatives on proliferation and osteogenic differentiation of menstrual blood-derived stem cells.

abstract：:Lactococcus lactis is industrially important microorganism used in many dairy fermentations. Numerous genes and gene expression signals from this organism have now been identified and characterized. Recently, several naturally occurring, inducible gene-expression systems have also been described in L. lactis. The main...

journal_title：Molecular biotechnology

authors： Djordjevic GM,Klaenhammer TR

更新日期：1998-04-01 00:00:00

abstract：:Papaya ringspot virus (PRSV) causes severe economic losses in both cucurbits and papaya throughout the tropics and subtropics. Development of PRSV-resistant transgenic plants faces a major hurdle in achieving resistance against geographically distinct isolates. One of the major reasons of failing to achieve the broad-...

journal_title：Molecular biotechnology

authors： Mangrauthia SK,Singh P,Praveen S

更新日期：2010-01-01 00:00:00

abstract：:Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...

journal_title：Molecular biotechnology

authors： Shamriz S,Ofoghi H

更新日期：2019-02-01 00:00:00

abstract：:Alternaria blight caused by Alternaria brassicicola (Schwein.) Wiltshire and A. brassicae (Berk.) Sacc., is one of the most important disease of rapeseed-mustard, characterized by the formation of spots on leaves, stem, and siliquae with premature defoliation and stunting of growth. These two species are very difficul...

journal_title：Molecular biotechnology

authors： Singh R,Kumar S,Kashyap PL,Srivastava AK,Mishra S,Sharma AK

更新日期：2014-11-01 00:00:00

abstract：:The Delta12 desaturase represents a diverse gene family in plants and is responsible for conversion of oleic acid (18:1) to linoleic acid (18:2). Several members of this family are known from plants like Arabidopsis and Soybean. Using primers from conserved C- and N-terminal regions, we have cloned a novel Delta12 des...

journal_title：Molecular biotechnology

authors： Khadake RM,Ranjekar PK,Harsulkar AM

更新日期：2009-06-01 00:00:00

abstract：:Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on ...

journal_title：Molecular biotechnology

authors： Shen WH,Escudero J,Hohn B

更新日期：1999-12-01 00:00:00

abstract：:Clinical molecular genetics has recently become recognized as a diagnostic discipline. This article covers the evolution, structure, and possible forward development of clinical molecular genetics. Topics covered include general test categories, introducing new tests, laboratory facilities, staffing and training, and ...

journal_title：Molecular biotechnology

authors： Elles R

更新日期：1997-10-01 00:00:00

abstract：:Association studies using genome scans to identify quantitative trait loci for multifactorial disorders, with anything approaching reasonable power, have been compromised by the need for a very dense array of genetic markers and large numbers of affected individuals. These requirements impose enormous burdens on the g...

journal_title：Molecular biotechnology

authors： Curran S,Hill L,O'Grady G,Turic D,Asherson P,Taylor E,Sham P,Craig I,Vaughan P

更新日期：2002-11-01 00:00:00

abstract：:In this study, human alpha-1,4-N-acetylglucosaminyltransferase (alpha4GnT) fused with GFP(uv) (GFP(uv)-alpha4GnT) was expressed using both a transformed cell system and silkworm larvae. A Tn-pXgp-GFP(uv)-alpha4GnT cell line, isolated after expression vector transfection, produced 106 mU/ml of alpha4GnT activity in sus...

journal_title：Molecular biotechnology

authors： Nakajima M,Kato T,Kanamasa S,Park EY

更新日期：2009-09-01 00:00:00

abstract：:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...

journal_title：Molecular biotechnology

authors： Ryu KH,Choi SH,Lee JS

更新日期：2000-01-01 00:00:00

abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...

journal_title：Molecular biotechnology

authors： Rotman G,Vanagaite L,Collins FS,Shiloh Y

更新日期：1995-04-01 00:00:00

abstract：:Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...

journal_title：Molecular biotechnology

authors： Díaz-Dinamarca DA,Jerias JI,Soto DA,Soto JA,Díaz NV,Leyton YY,Villegas RA,Kalergis AM,Vásquez AE

更新日期：2018-03-01 00:00:00

abstract：:The aim of this study was to evaluate the usefulness of replicating but non disseminating adenovirus vectors (AdVs) as vaccine vector using human rotavirus (HRV) as a model pathogen. HRV VP7, VP4, or VP4Δ (N-terminal 336 amino acids of VP4) structural proteins as well as the VP4Δ::VP7 chimeric fusion protein were expr...

journal_title：Molecular biotechnology

authors： Girard A,Roques E,St-Louis MC,Massie B,Archambault D

更新日期：2013-07-01 00:00:00

abstract：:D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...

journal_title：Molecular biotechnology

authors： Li P,Li X,Wen Z,Dong R,Li K,Fan H,Wang R,Wang J,Lv M

更新日期：2020-08-01 00:00:00

abstract：:Efficient display of antibody on filamentous phage M13 coat is crucial for successful biopanning selections. We applied a directed evolution strategy to improve the oligovalent display of a poorly behaving Fab fragment fused to phage gene-3 for minor coat protein (g3p). The Fab displaying clones were enriched from a r...

journal_title：Molecular biotechnology

authors： Huovinen T,Sanmark H,Ylä-Pelto J,Vehniäinen M,Lamminmäki U

更新日期：2010-03-01 00:00:00

abstract：:The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...

journal_title：Molecular biotechnology

authors： Devon RS,Brookes AJ

更新日期：1996-06-01 00:00:00

abstract：:N-Succinyl-amino acid racemase (NSAAR), long referred to as N-acyl- or N-acetyl-amino acid racemase, is an enolase superfamily member whose biotechnological potential was discovered decades ago, due to its use in the industrial dynamic kinetic resolution methodology first known as "Acylase Process". In previous works,...

journal_title：Molecular biotechnology

authors： Soriano-Maldonado P,Andújar-Sánchez M,Clemente-Jiménez JM,Rodríguez-Vico F,Las Heras-Vázquez FJ,Martínez-Rodríguez S

更新日期：2015-05-01 00:00:00

abstract：:This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be den...

journal_title：Molecular biotechnology

authors： Molloy PE,Graham BM,Cupit PM,Grant SD,Porter AJ,Cunningham C

更新日期：1995-12-01 00:00:00

abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...

journal_title：Molecular biotechnology

authors： do Nascimento NC,Menguer PK,Sperotto RA,de Almeida MR,Fett-Neto AG

更新日期：2013-05-01 00:00:00

abstract：:A bacterial artificial chromosome library of the causal agent of the Black Sigatoka leaf spot disease of banana and plantain, Mycosphaerella fijiensis, has been constructed using a non-sphaeroplasting technique and characterized using both homologous and heterologous probes. After first and a second size selection of ...

journal_title：Molecular biotechnology

authors： Canto-Canché B,Guillén-Maldonado DK,Peraza-Echeverría L,Conde-Ferráez L,James-Kay A

更新日期：2007-05-01 00:00:00

abstract：:An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could repr...

journal_title：Molecular biotechnology

authors： Wang Z,Wang HY,Feng H

更新日期：2013-01-01 00:00:00

abstract：:This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia...

journal_title：Molecular biotechnology

authors： Meneghetti S,Costacurta A,Frare E,Da Rold G,Migliaro D,Morreale G,Crespan M,Sotés V,Calò A

更新日期：2011-07-01 00:00:00

abstract：:Heat shock proteins (HSPs) are upregulated in response to stress and play a protective function in refolding of cellular proteins. In hypertension, the heart is a vital organ that requires examination and investigation, and primary induction of HSPs is predominantly effected. Hypertension results from osmotic imbalanc...

journal_title：Molecular biotechnology

authors： Mala JG,Takeuchi S

更新日期：2009-06-01 00:00:00

abstract：:Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...

journal_title：Molecular biotechnology

authors： Naik V,Kardani J,Roy I

更新日期：2016-04-01 00:00:00

abstract：:Recent human clinical trials results demonstrated successful treatment for certain genetic forms of cystic fibrosis (CF). To extend treatment opportunities to those afflicted with other genetic forms of CF disease, structural and biophysical characterization of CF transmembrane conductance regulator (CFTR) is urgently...

journal_title：Molecular biotechnology

authors： Hildebrandt E,Mulky A,Ding H,Dai Q,Aleksandrov AA,Bajrami B,Diego PA,Wu X,Ray M,Naren AP,Riordan JR,Yao X,DeLucas LJ,Urbatsch IL,Kappes JC

更新日期：2015-05-01 00:00:00

abstract：:Virus-like particles (VLPs) have vast potential for applications in nanoscience and nanomedicine. These biological nanoparticles may be used for medical imaging, vaccination, or tissue-specific delivery of drugs or other bioactive molecules. VLPs of Human parvovirus B19 (B19 V) can be assembled in vitro from the recom...

journal_title：Molecular biotechnology

authors： Sánchez-Rodríguez SP,Enrriquez-Avila JV,Soto-Fajardo JM,Peña-Montes C,Bustos-Jaimes I

更新日期：2015-04-01 00:00:00

abstract：:Tall fescue plants are widely exposed to white grubs. Regarding the rate of damage caused by the white grubs to tall fescue and difficulty of its ecological and economical control, production of resistant cultivars is a priority. In this experiment, for the first time, we report production of transgenic lines resistan...

journal_title：Molecular biotechnology

authors： Hosseini HR,Salehi H,Alichi M

更新日期：2019-07-01 00:00:00

abstract：:The advent of recombinant antibody technology led to an enormous revival in the use of antibodies as diagnostic and therapeutic tools for fighting cancer. This review provides a brief historical sketch of the development of recombinant antibodies for the diagnosis and immunotherapy of cancer and summarizes the most si...

journal_title：Molecular biotechnology

authors： Krauss J

更新日期：2003-09-01 00:00:00

abstract：:Molecular biological methods that use antibodies and nucleic acids to detect specific foodborne bacterial pathogens were scarcely known a decade and a half ago. Few scientists could have predicted that these tools of basic research would come to dominate the field of food diagnostics. Today, a large number of cleverly...

journal_title：Molecular biotechnology

authors： Feng P

更新日期：1997-06-01 00:00:00

abstract：:A duplex real-time PCR assay with melting curve analysis, using the EvaGreen fluorescence dye, was developed for rapid and reliable identification of bovine and caprine in ruminant feeds. The method merges the use of bovine (Bos taurus) and caprine (Capra hircus) specific primers that amplify small fragments (bovine 9...

journal_title：Molecular biotechnology

authors： Safdar M,Junejo Y,Arman K,Abasıyanık MF

更新日期：2014-08-01 00:00:00