Abstract:
:Association studies using genome scans to identify quantitative trait loci for multifactorial disorders, with anything approaching reasonable power, have been compromised by the need for a very dense array of genetic markers and large numbers of affected individuals. These requirements impose enormous burdens on the genotyping capacity for most laboratories. DNA pooling has been proposed as a possible approach to reduce genotyping costs and effort. We report on the application of the SNaPIT technology to evaluate allele frequencies in pooled DNA samples and conclude that it offers a cost effective, efficient and accurate estimator and provides several advantages over competing technologies in this regard.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Curran S,Hill L,O'Grady G,Turic D,Asherson P,Taylor E,Sham P,Craig I,Vaughan Pdoi
10.1385/mb:22:3:253keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
253-62issue
3eissn
1073-6085issn
1559-0305pii
MB:22:3:253journal_volume
22pub_type
杂志文章abstract::Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher's disease, the most common inherited storage disorder. Treatment consists of enzyme replacement therapy by the administration of recombinant GCR produced in Chinese hamster ovary cells. The production of anti-GCR antibodies has already been...
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pub_type: 杂志文章
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journal_title:Molecular biotechnology
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abstract::The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticu...
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journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
abstract::This review outlines the various methods available for submitting sequence data to the EMBL Nucleotide Sequence Database. Depending on the type of sequence data and the facilities available to the submitter, one method may be more suitable than another. Recent developments have been the World Wide Web submission tool,...
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pub_type: 杂志文章,评审
doi:10.1007/BF02762322
更新日期:1996-08-01 00:00:00
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journal_title:Molecular biotechnology
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pub_type: 杂志文章,评审
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更新日期:2003-11-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1007/s12033-019-00186-2
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pub_type: 杂志文章
doi:10.1385/MB:12:3:237
更新日期:1999-10-01 00:00:00
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journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02760779
更新日期:1997-12-01 00:00:00
abstract::Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9735-3
更新日期:2014-05-01 00:00:00
abstract::Small ubiquitin-related modifier (SUMO) fusion system has been shown to be efficient for enhancing expression and preventing degradation of the target protein. We showed herein that SUMO fusion to human keratinocyte growth factor 2 (hKGF-2) gene was feasible and it significantly enhanced protein expression and its eff...
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journal_title:Molecular biotechnology
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更新日期:2010-11-01 00:00:00
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pub_type: 杂志文章
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