Abstract:
:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary catabolites. In this study, an efficient method was developed to directly isolate pure LMW RNA from pear peel, a material rich in polyphenolics that is covered with a layer of wax. The method was based on polyethylene glycol (PEG) precipitation combining CTAB buffer which is often used to isolate RNA from polysaccharide-rich and polyphenolics-rich materials. The entire procedure could be completed within 6 h and many samples could be processed at the same time. Few and common chemicals are used with this method. Hence, it could be used as an ordinary method in the laboratory. The developed method was further tested by isolating LMW RNA from Arabidopsis. Using the isolated LMW RNA samples, microRNAs were successfully detected and characterized.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Wang XW,Xiong AS,Yao QH,Zhang Z,Qiao YSdoi
10.1007/s12033-009-9204-6subject
Has Abstractpub_date
2010-01-01 00:00:00pages
61-5issue
1eissn
1073-6085issn
1559-0305journal_volume
44pub_type
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