Abstract:
:Many preclinical studies have shown RNA interference (RNAi) as a new promising way to treat various human diseases including cancer and virus infection and there is an increasing demand for the large-scale preparation of short interfering RNAs (siRNAs) at low cost. Data are accumulating to show that endoribonuclease-prepared siRNAs (esiRNAs) are superior to chemically synthesized siRNAs in terms of expense, efficiency, and specificity. Yet all procedures available for esiRNA purification were designed to produce small amount of siRNAs for laboratory use. In this article, a new method of purification of esiRNAs based on ion exchange chromatography and size exclusion chromatography is reported. The esiRNAs prepared with this method are shown here to be of high purity and specifically suppress homologous gene expression without activating interferon response and with higher efficiency than chemically synthesized siRNAs. We can expect that the new method can be scaled up easily to provide large quantities of esiRNAs to meet the requirement of preclinical and clinical studies.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Xuan B,Qian Z,Tan C,Min T,Shen S,Huang Wdoi
10.1385/MB:31:3:203keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
203-9issue
3eissn
1073-6085issn
1559-0305pii
MB:31:3:203journal_volume
31pub_type
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