Abstract:
:Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood progenitors, particularly plastic adherent or CD14+ monocytes, in media supplemented with GM-CSF and IL-4. These DC may then be matured, if desired, and loaded with antigen, such as tumor-associated peptides, prior to administration. We describe the scheme that we are currently using to generate peptide-loaded dendritic cells for our clinical trials of cancer immunotherapy.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Morse MA,Clay T,Colling K,Lyerly HKdoi
10.1385/MB:25:1:95keywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
95-9issue
1eissn
1073-6085issn
1559-0305pii
MB:25:1:95journal_volume
25pub_type
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