Abstract:
:The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those stimuli are transmitted to the cytosolic and nuclear targets in a rapid and specific manner. In the last few years it has become clear that the specificity and the rapid function of the ERK cascade is largely determined by protein-protein interactions with various signaling components and substrates. This review describes interactions of ERK with its immediate regulators, scaffold proteins, substrates, and localizing proteins, and shows their involvement in the functioning of the ERK cascade. Understanding the full scope of ERK-interactions is important for the development of new drugs for the treatment of cancer and other diseases.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Chuderland D,Seger Rdoi
10.1385/MB:29:1:57keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
57-74issue
1eissn
1073-6085issn
1559-0305pii
MB:29:1:57journal_volume
29pub_type
杂志文章,评审abstract::Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0065-8
更新日期:2018-03-01 00:00:00
abstract::In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00194-2
更新日期:2019-09-01 00:00:00
abstract::Angiogenesis has been considered an important target for cancer therapy. The inhibition of angiogenesis represents a promising strategy for anti-cancer treatment, tumor growth inhibition, and metastasis. Vasostatin 30 (Vs30), and the 14.1 kDa vasoinhibin (Vi-II-14.1) are two peptides with remarkable anti-tumor and ant...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9642-4
更新日期:2013-07-01 00:00:00
abstract::Lactic acid bacteria (LAB) are functional starter cultures in food and dairy industry and are also regarded as power houses for bioprocess and fermentation technology. Due to extensive applications in food and medical applications, intensive research and developmental activities are going on throughout the world to un...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-019-00186-2
更新日期:2019-08-01 00:00:00
abstract::In this article, four types of standards were assessed in a SYBR Green-based real-time PCR procedure for the quantification of Staphylococcus aureus (S. aureus) in DNA samples. The standards were purified S. aureus genomic DNA (type A), circular plasmid DNA containing a thermonuclease (nuc) gene fragment (type B), DNA...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9417-3
更新日期:2012-01-01 00:00:00
abstract::Human papillomavirus (HPV) is the causal agent of cervical cancer, one of the most common causes of death in women worldwide, and its E7 antigen is the major candidate for a therapeutic vaccine. The large scale production of E7 by molecular farming that would lead to the development of a safe and inexpensive vaccine i...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9781-x
更新日期:2014-11-01 00:00:00
abstract::Tall fescue plants are widely exposed to white grubs. Regarding the rate of damage caused by the white grubs to tall fescue and difficulty of its ecological and economical control, production of resistant cultivars is a priority. In this experiment, for the first time, we report production of transgenic lines resistan...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00183-5
更新日期:2019-07-01 00:00:00
abstract::Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0094-3
更新日期:2018-08-01 00:00:00
abstract::Over the last decade transgenic mouse models have become a common experimental tool for unraveling gene function. During this time there has been a growing expectation that transgenes resemble the in vivo state as much as possible. To this end, a preference away from heterologous promoters has emerged, and transgene c...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:29:2:153
更新日期:2005-02-01 00:00:00
abstract::A synthetic gene encoding a single chain Fv fragment of an antibody directed against the nuclear inclusion a (NIa) protein of potato virus Y (PVY) was used to transform two commercial potato cultivars (Claustar and BF15). The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavag...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:33:2:133
更新日期:2006-06-01 00:00:00
abstract::Identification of expressed sequence tags (ESTs) in large genomic segments is an important step in positional cloning and genomic mapping studies. A simple and efficient polymerase chain reaction (PCR)-based approach is described here to identify coding sequences in large genomic fragments of DNA cloned in vectors suc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02760779
更新日期:1997-12-01 00:00:00
abstract::The rhoptry 2 protein (Rop2) is an interesting protein of Toxoplasma gondii that is involved in the parasite invasion of host cell, it has three T-cell epitopes and high antigenic value. However, the expression of Rop2 as a recombinant protein in Escherichia coli is not an easy task, showing low levels of expression o...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:18:3:269
更新日期:2001-07-01 00:00:00
abstract::The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticu...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9486-3
更新日期:2012-10-01 00:00:00
abstract::De novo design provides an in silico toolkit for the design of novel small molecular structures to a set of specified structural constraints. With the avalanche of bioinformatics data, de novo design is ideally suited for exploring molecules that could be useful for chemical genomics. The design process involves manip...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-007-0037-x
更新日期:2007-11-01 00:00:00
abstract::DNA damage is one of the most impactful events in living organisms, leading to DNA sequence changes (mutation) and disruption of biological processes. A study has identified a protein called Damage Suppressor Protein (Dsup) in the tardigrade Ramazzotius varieornatus that has shown to reduce the effects of radiation da...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00273-9
更新日期:2020-12-01 00:00:00
abstract::It is difficult to obtain precise and accurate estimates of the true mycotoxin concentration of a bulk lot when using a mycotoxin-sampling plan that measures the concentration in a small portion of the bulk lot. A mycotoxin-sampling plan is defined by a mycotoxin test procedure and a defined accept/reject limit. A myc...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:23:1:61
更新日期:2003-01-01 00:00:00
abstract::The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orient...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0046-3
更新日期:2018-01-01 00:00:00
abstract::Considering the fact that Prays oleae is one of the most pathogenic insects to the olive tree in the Mediterranean basin, particularly in Tunisia, the mode of action of Cry insecticidal toxins of Bacillus thuringiensis kurstaki in Prays oleae midgut was investigated. The proteolysis of Bacillus thuringiensis delta-end...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02686109
更新日期:2007-02-01 00:00:00
abstract::The biosensor field has grown enormously since the first demonstration of the biosensor concept by Leland C. Clark, Jr. in 1962. Today's biosensor market is dominated by glucose biosensors, mass-produced enzyme electrodes for the rapid self-diagnosis of blood glucose levels by diabetes sufferers. Here we take a histor...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:32:3:249
更新日期:2006-03-01 00:00:00
abstract::The mapping of genetic loci within organisms has been accelerated by the advent of Radiation Hybrid (RH) panels. These panels are available for humans and non-humans including mice, baboon, rat, and canine. This article contains a general protocol for the use of the Genebridge 4 whole genome RH panel to map a human lo...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:19:2:205
更新日期:2001-10-01 00:00:00
abstract::This article describes the construction, expression, and purification of RNase single-chain antibody fusion proteins. To construct a fusion protein, the gene for each moiety, the RNase and the binding ligand, is modified separately to contain complementary DNA encoding a 13 amino acid spacer that separates the RNase f...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:20:1:063
更新日期:2002-01-01 00:00:00
abstract::Heat shock proteins (HSPs) are upregulated in response to stress and play a protective function in refolding of cellular proteins. In hypertension, the heart is a vital organ that requires examination and investigation, and primary induction of HSPs is predominantly effected. Hypertension results from osmotic imbalanc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9144-1
更新日期:2009-06-01 00:00:00
abstract::Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials us...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:21:1:057
更新日期:2002-05-01 00:00:00
abstract::Human adipose tissue-derived stromal cells (ADSCs) are being evaluated for cardiovascular repair. We developed an ex vivo method for producing angiogenic ADSCs transduced with a self-inactivating lentiviral vector (LV) expressing the enhanced green fluorescence protein (EGFP) from an internal cytomegalovirus (CMV) pro...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9537-4
更新日期:2013-05-01 00:00:00
abstract::Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field ...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:26:2:147
更新日期:2004-02-01 00:00:00
abstract::Several mutations were introduced into the Cry1Ac toxin gene, resulting in four variants with altered sequences that were responsible for low expression of the toxin in transgenic plants. These variants were as follows: V1, with modified three A/T-rich regions, including the first signal of transcription termination; ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/mb:26:1:17
更新日期:2004-01-01 00:00:00
abstract::In this study cationic β-cyclodextrin-chitosan-mediated nanoparticles were used to transfer pmCherry-C1 into glioblastoma cells and their transfection efficiency were compared to lipofectamine and electroporation. Physicochemical characteristics of nanoparticles were evaluated by photon correlation spectroscopy and sc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9927-0
更新日期:2016-04-01 00:00:00
abstract::A sandwich-type enzyme-linked immunosorbent assay (ELISA) was established for monitoring the secretion of ZZ-fusion proteins. Two antibodies, a monoclonal mouse anti-human proinsulin and a rabbit anti-bovine IgG (strongly binding to the ZZ-domain), were used to quantify the secretion of recombinant human ZZ-proinsulin...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:19:3:239
更新日期:2001-11-01 00:00:00
abstract::Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9735-3
更新日期:2014-05-01 00:00:00
abstract::A duplex real-time PCR assay with melting curve analysis, using the EvaGreen fluorescence dye, was developed for rapid and reliable identification of bovine and caprine in ruminant feeds. The method merges the use of bovine (Bos taurus) and caprine (Capra hircus) specific primers that amplify small fragments (bovine 9...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9756-y
更新日期:2014-08-01 00:00:00