Novel fusion protein derived from vasostatin 30 and vasoinhibin II-14.1 potently inhibits coronary endothelial cell proliferation.

abstract：:Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...

journal_title：Molecular biotechnology

authors： Díaz-Dinamarca DA,Jerias JI,Soto DA,Soto JA,Díaz NV,Leyton YY,Villegas RA,Kalergis AM,Vásquez AE

更新日期：2018-03-01 00:00:00

abstract：:In this review I briefly describe the technique of protein engineering and indicate how the present state of knowledge allows proteins to be mutated to increase or decrease stability. I discuss experiments on both model proteins and those of relevance to the food industry and show how hydrophobic forces are a major dr...

journal_title：Molecular biotechnology

authors： Goodenough PW

更新日期：1995-10-01 00:00:00

abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...

journal_title：Molecular biotechnology

authors： do Nascimento NC,Menguer PK,Sperotto RA,de Almeida MR,Fett-Neto AG

更新日期：2013-05-01 00:00:00

abstract：:The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...

journal_title：Molecular biotechnology

authors： Devon RS,Brookes AJ

更新日期：1996-06-01 00:00:00

abstract：:An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained b...

journal_title：Molecular biotechnology

authors： Weber K,Bolander ME,Sarkar G

更新日期：1998-02-01 00:00:00

abstract：:Plants synthesize amino acids by collateral metabolic pathways using primary elements carbon and oxygen from air, hydrogen from water in soil and nitrogen from soil. Following synthesis, amino acids are immediately used for metabolism, transient storage or transported to the phloem. Different families of transporters ...

journal_title：Molecular biotechnology

authors： Chakraborty N,Besra A,Basak J

更新日期：2020-03-01 00:00:00

abstract：:As the use of adenoviral vectors in gene therapy protocols increases, there is a corresponding need for rapid, accurate, and reproducible titer methods. Multiple methods currently exist for determining titers of recombinant adenoviral vector, including optical absorbance, electron microscopy, fluorescent focus assay, ...

journal_title：Molecular biotechnology

authors： Hitt DC,Booth JL,Dandapani V,Pennington LR,Gimble JM,Metcalf J

更新日期：2000-03-01 00:00:00

abstract：:Enterotoxigenic strains of Escherichia coli produce a heat-labile holotoxin (LT), which causes diarrhea. We engineered corn seeds to produce LT-B, the nontoxic subunit of LT, to serve as a plant-derived vaccine to traveler's diarrhea and as an adjuvant for co-administered proteins. We previously demonstrated that a st...

journal_title：Molecular biotechnology

authors： Karaman S,Cunnick J,Wang K

更新日期：2006-01-01 00:00:00

abstract：:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...

journal_title：Molecular biotechnology

authors： Kalwy S,Rance J,Young R

更新日期：2006-10-01 00:00:00

abstract：:This article describes the construction, expression, and purification of RNase single-chain antibody fusion proteins. To construct a fusion protein, the gene for each moiety, the RNase and the binding ligand, is modified separately to contain complementary DNA encoding a 13 amino acid spacer that separates the RNase f...

journal_title：Molecular biotechnology

authors： Newton DL,Rybak SM

更新日期：2002-01-01 00:00:00

abstract：:Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...

journal_title：Molecular biotechnology

authors： Noble JE,Knight AE,Reason AJ,Di Matola A,Bailey MJ

更新日期：2007-10-01 00:00:00

abstract：:Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ab...

journal_title：Molecular biotechnology

authors： Szurgot I,Jedynak M,Podsiadla-Bialoskorska M,Piwowarski J,Szolajska E,Chroboczek J

更新日期：2015-06-01 00:00:00

abstract：:The low economic profits of feather recycling lead that the large amount of feathers is currently discarded in China. To convert feather hydrolysates into GSH with high values, surface display of the bifunctional glutathione synthetase encoded by gcsgs from Streptococcus thermophilus on Saccharomyces cerevisiae and th...

journal_title：Molecular biotechnology

authors： Qiu Z,Tan H,Zhou S,Cao L

更新日期：2014-08-01 00:00:00

abstract：:IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalyti...

journal_title：Molecular biotechnology

authors： Mitsuda Y,Planque S,Hara M,Kyle R,Taguchi H,Nishiyama Y,Paul S

更新日期：2007-06-01 00:00:00

abstract：:Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher's disease, the most common inherited storage disorder. Treatment consists of enzyme replacement therapy by the administration of recombinant GCR produced in Chinese hamster ovary cells. The production of anti-GCR antibodies has already been...

journal_title：Molecular biotechnology

authors： Novo JB,Oliveira ML,Magalhães GS,Morganti L,Raw I,Ho PL

更新日期：2010-11-01 00:00:00

abstract：:Several mutations were introduced into the Cry1Ac toxin gene, resulting in four variants with altered sequences that were responsible for low expression of the toxin in transgenic plants. These variants were as follows: V1, with modified three A/T-rich regions, including the first signal of transcription termination; ...

journal_title：Molecular biotechnology

authors： Misztal LH,Mostowska A,Skibinska M,Bajsa J,Musial WG,Jarmolowski A

更新日期：2004-01-01 00:00:00

abstract：:Ageing is a complex and progressive phenomenon, during which the accumulation of morphological and chemical changes seriously compromises the capacity of the cells to proliferate and fulfil their biological tasks. The increase in the average age of the world population, associated with a higher occurrence of age-relat...

journal_title：Molecular biotechnology

authors： Tito A,Barbulova A,Zappelli C,Leone M,Ruvo M,Mercurio FA,Chambery A,Russo R,Colucci MG,Apone F

更新日期：2019-03-01 00:00:00

abstract：:The biosensor field has grown enormously since the first demonstration of the biosensor concept by Leland C. Clark, Jr. in 1962. Today's biosensor market is dominated by glucose biosensors, mass-produced enzyme electrodes for the rapid self-diagnosis of blood glucose levels by diabetes sufferers. Here we take a histor...

journal_title：Molecular biotechnology

authors： Newman JD,Setford SJ

更新日期：2006-03-01 00:00:00

abstract：:Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...

journal_title：Molecular biotechnology

authors： Shamriz S,Ofoghi H

更新日期：2019-02-01 00:00:00

abstract：:The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...

journal_title：Molecular biotechnology

authors： Isik N,White L,Barnes R,Poynton CJ,Mills KI

更新日期：2003-07-01 00:00:00

abstract：:It is difficult to obtain precise and accurate estimates of the true mycotoxin concentration of a bulk lot when using a mycotoxin-sampling plan that measures the concentration in a small portion of the bulk lot. A mycotoxin-sampling plan is defined by a mycotoxin test procedure and a defined accept/reject limit. A myc...

journal_title：Molecular biotechnology

authors： Whitaker TB

更新日期：2003-01-01 00:00:00

abstract：:The gram-negative anaerobic oral bacterium Porphyromonas gingivalis initiates periodontal disease through fimbrial attachment to saliva-coated oral surfaces. To study the effects of immunomodulation on enhancement of subunit vaccination, the expression in E. coli and immunogenicity of P. gingivalis fimbrial protein (F...

journal_title：Molecular biotechnology

authors： Kim TG,Huy NX,Kim MY,Jeong DK,Jang YS,Yang MS,Langridge WH,Lee JY

更新日期：2009-02-01 00:00:00

abstract：:Menstrual blood has been recognized as an easily accessible and inexpensive source of stem cells, in recent years. To establish a safe and efficient protocol for development of menstrual blood-derived stem cells (MenSCs) into osteoblasts, the effect of substitution of fetal bovine serum (FBS) with human platelet deriv...

journal_title：Molecular biotechnology

authors： Kazemnejad S,Najafi R,Zarnani AH,Eghtesad S

更新日期：2014-03-01 00:00:00

abstract：:CD151 is an abundantly expressed eukaryotic transmembrane protein on the cell surface. It is involved in cell adhesion, angiogenesis and signal transduction as well in disease conditions such as cancer and viral infections. However, the molecular mechanism of CD151 activation is poorly understood due to the lack of st...

journal_title：Molecular biotechnology

authors： Purushothaman G,Thiruvenkatam V

更新日期：2019-12-01 00:00:00

abstract：:We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...

journal_title：Molecular biotechnology

authors： Todorov T,Todorova A,Georgieva B,Mitev V

更新日期：2010-06-01 00:00:00

abstract：:Water-soluble quinoprotein glucose dehydrogenase (PQQGDH-B) is a dimeric enzyme whose application for glucose sensing is the focus of much attention. We attempted to increase the thermal stability of PQQGDH-B by introducing a disulfide bond at the dimer interface. The Ser residue at position 415 was selected for subst...

journal_title：Molecular biotechnology

authors： Igarashi S,Sode K

更新日期：2003-06-01 00:00:00

abstract：:Lactic acid bacteria (LAB) are functional starter cultures in food and dairy industry and are also regarded as power houses for bioprocess and fermentation technology. Due to extensive applications in food and medical applications, intensive research and developmental activities are going on throughout the world to un...

journal_title：Molecular biotechnology

authors： Vivek N,Hazeena SH,Rajesh RO,Godan TK,Anjali KB,Nair LM,Mohan B,Nair SC,Sindhu R,Pandey A,Binod P

更新日期：2019-08-01 00:00:00

abstract：:Megaprimer-based methodology has been widely applied in site-directed mutagenesis, but rarely used in gene splicing. In this article, we describe a modification of the megaprimer PCR method, which can efficiently create and amplify a specific ligated chimeric gene segment in a PCR reaction and under a common PCR progr...

journal_title：Molecular biotechnology

authors： Chen JR,Lü JJ,Wang HF

更新日期：2008-11-01 00:00:00

abstract：:Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated temperatures, including enhanced reaction rates, increased substrate solubility and decreased risks of contamination. Furthermore, thermophilic ...

journal_title：Molecular biotechnology

authors： Mandelli F,Gonçalves TA,Gandin CA,Oliveira AC,Oliveira Neto M,Squina FM

更新日期：2016-11-01 00:00:00

abstract：:Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achieveme...

journal_title：Molecular biotechnology

authors： Li R,Kang G,Hu M,Huang H

更新日期：2019-01-01 00:00:00