Toward more efficient protein expression: keep the message simple.


:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a complex macromolecule, namely an antibody. When applied to the heavy and light chain genes of our model antibody, we found that greater numbers of high-producing transfectants as well as increased levels of protein production were observed (approximately 1.5-fold). In this test model, production was improved even though the antibody has previously been demonstrated to give high expression in stably transfected cells (up to 5 g/L in bioreactors). Because the parental heavy chain sequence contained introns, and the process of gene optimization is most efficiently performed on sequences without introns, we demonstrated that removal of introns in the coding sequence had no effect on the quantity of antibody produced. All constructs were evaluated using Lonza's glutamine synthetase gene expression vectors in Chinese hamster ovary cells. Our findings suggest that significant improvements in product yields can be achieved by gene optimization, which may facilitate the processing and translation of gene transcripts.


Mol Biotechnol


Molecular biotechnology


Kalwy S,Rance J,Young R




Has Abstract


2006-10-01 00:00:00














  • Genomics of helper component proteinase reveals effective strategy for papaya ringspot virus resistance.

    abstract::Papaya ringspot virus (PRSV) causes severe economic losses in both cucurbits and papaya throughout the tropics and subtropics. Development of PRSV-resistant transgenic plants faces a major hurdle in achieving resistance against geographically distinct isolates. One of the major reasons of failing to achieve the broad-...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Mangrauthia SK,Singh P,Praveen S

    更新日期:2010-01-01 00:00:00

  • Flavonoid compounds in maintenance of prostate health and prevention and treatment of cancer.

    abstract::Compounds based on a flavonoid (di-phenolic) ring structure are emerging as a potentially important new class of pharmaceutical compounds with a broad range of biological activities, most prominent of which are their potential role as anticancer agents. These compounds exert a wide range of upregulating and downregula...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章,评审


    authors: Brown DM,Kelly GE,Husband AJ

    更新日期:2005-07-01 00:00:00

  • Polyamines. An overview.

    abstract::The polyamines spermine, spermidine, and putrescine are small organic molecules one or more of which are present in all living organisms. Many natural products contain polyamine residues. Polyamines are synthesized by a highly regulated pathway from arginine or ornithine and also can be transported in and out of cells...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章,评审


    authors: Morgan DM

    更新日期:1999-06-01 00:00:00

  • Expression and characterization of biologically active human Fas ligand produced in CHO cells.

    abstract::We describe an expression system for high-yield production of recombinant soluble human FasL (rsh- FasL) in CHO cells. After one round of selection for gene amplification, cell lines producing rsh-FasL up to 60 microg/L x 10(6) cells in 24 h were obtained. Cell lines were grown in protein-free medium as suspension cul...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Zappitelli S,D'Alatri L,Ciucci A,Raucci G,Faiella A,Gabrielli M,Parlani M,Bressan A,Maggi CA,Goso C,Rotondaro L

    更新日期:2003-03-01 00:00:00

  • New insights into protein-DNA interactions obtained by electron microscopy.

    abstract::The electron microscopic study of DNA-protein complexes can yield valuable information that is often not easily available by other methods. In this article we give a number of examples that were chosen to illustrate the utility of electron microscopy. Along with the strategy used are protocols that allow such experime...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Schnos M,Inman RB

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  • Impact of molecular biology on the detection of foodborne pathogens.

    abstract::Molecular biological methods that use antibodies and nucleic acids to detect specific foodborne bacterial pathogens were scarcely known a decade and a half ago. Few scientists could have predicted that these tools of basic research would come to dominate the field of food diagnostics. Today, a large number of cleverly...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章,评审


    authors: Feng P

    更新日期:1997-06-01 00:00:00

  • Cationic β-Cyclodextrin-Chitosan Conjugates as Potential Carrier for pmCherry-C1 Gene Delivery.

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Eslaminejad T,Nematollahi-Mahani SN,Ansari M

    更新日期:2016-04-01 00:00:00

  • Expression of a synthetic cholera toxin B subunit in tobacco using ubiquitin promoter and bar gene as a selectable marker.

    abstract::A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered to modify the codon usage to that of tobacco plant genes. The gene was then cloned into a plant expression vect...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Kang TJ,Kim BG,Yang JY,Yang MS

    更新日期:2006-02-01 00:00:00

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Martinon A,Cronin UP,Wilkinson MG

    更新日期:2012-01-01 00:00:00

  • Simultaneous EGFP and tag labeling of the β7 subunit for live imaging and affinity purification of functional human proteasomes.

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Kulichkova VA,Artamonova TO,Zaykova JJ,Ermolaeva JB,Khodorkovskii MA,Barlev NA,Tomilin AN,Tsimokha AS

    更新日期:2015-01-01 00:00:00

  • Studying the effects of actin cytoskeletal destabilization on cell cycle by cofilin overexpression.

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    journal_title:Molecular biotechnology

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    authors: Lee YJ,Keng PC

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  • IMGT, the International ImMunoGeneTics Information System for Immunoinformatics : methods for querying IMGT databases, tools, and web resources in the context of immunoinformatics.

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Lefranc MP

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  • Virus crystallography.

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章,评审


    authors: Fry EE,Grimes J,Stuart DI

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  • Enantioselective nitrilase from Pseudomonas putida: cloning, heterologous expression, and bioreactor studies.

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Banerjee A,Dubey S,Kaul P,Barse B,Piotrowski M,Banerjee UC

    更新日期:2009-01-01 00:00:00

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Endo S,Tomimoto Y,Shimizu H,Taniguchi Y,Onizuka T

    更新日期:2006-07-01 00:00:00

  • Ribosome Display: A Potent Display Technology used for Selecting and Evolving Specific Binders with Desired Properties.

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章,评审


    authors: Li R,Kang G,Hu M,Huang H

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Todorov T,Todorova A,Georgieva B,Mitev V

    更新日期:2010-06-01 00:00:00

  • Trehalose-Induced Structural Transition Accelerates Aggregation of α-Synuclein.

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    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Naik V,Kardani J,Roy I

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  • Cloning of a novel omega-6 desaturase from flax (Linum usitatissimum L.) and its functional analysis in Saccharomyces cerevisiae.

    abstract::The Delta12 desaturase represents a diverse gene family in plants and is responsible for conversion of oleic acid (18:1) to linoleic acid (18:2). Several members of this family are known from plants like Arabidopsis and Soybean. Using primers from conserved C- and N-terminal regions, we have cloned a novel Delta12 des...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Khadake RM,Ranjekar PK,Harsulkar AM

    更新日期:2009-06-01 00:00:00

  • Enhancement of Production of D-Glucosamine in Escherichia coli by Blocking Three Pathways Involved in the Consumption of GlcN and GlcNAc.

    abstract::D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Li P,Li X,Wen Z,Dong R,Li K,Fan H,Wang R,Wang J,Lv M

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  • Molecular Cloning of an Amino Acid Permease Gene and Structural Characterization of the Protein in Common Bean (Phaseolus vulgaris L.).

    abstract::Plants synthesize amino acids by collateral metabolic pathways using primary elements carbon and oxygen from air, hydrogen from water in soil and nitrogen from soil. Following synthesis, amino acids are immediately used for metabolism, transient storage or transported to the phloem. Different families of transporters ...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章,收录出版


    authors: Chakraborty N,Besra A,Basak J

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  • A simple program to calculate codon bias index.

    abstract::A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Wang TT,Cheng WC,Lee BH

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  • Gene transfer by electroporation.

    abstract::Electroporation of cells in the presence of DNA is widely used for the introduction of transgenes either stably or transiently into bacterial, fungal, animal, and plant cells. A review of the literature shows that electroporation parameters are often reported in an incomplete or incorrect manner, forcing researchers t...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章,评审


    authors: Lurquin PF

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  • Advances in pharmacogenomic research and development.

    abstract::Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technol...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章,评审


    authors: McCarthy JJ

    更新日期:2003-11-01 00:00:00

  • Validation of biopharmaceutical purification processes for virus clearance evaluation.

    abstract::Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials us...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章,评审


    authors: Darling A

    更新日期:2002-05-01 00:00:00

  • Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase.

    abstract::Water-soluble quinoprotein glucose dehydrogenase (PQQGDH-B) is a dimeric enzyme whose application for glucose sensing is the focus of much attention. We attempted to increase the thermal stability of PQQGDH-B by introducing a disulfide bond at the dimer interface. The Ser residue at position 415 was selected for subst...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Igarashi S,Sode K

    更新日期:2003-06-01 00:00:00

  • Use of a short A/T-rich cassette for enhanced expression of cloned genes in Escherichia coli.

    abstract::A short (43-bp) A/T-rich stretch of DNA located in the intergenic region between the baiA2 and baiF genes from Eubacterium sp. strain VPI 12708 was amplified by polymerase chain reaction (PCR) and inserted in front of the Shine-Dalgarno (SD) sequences of three inefficiently-expressed Eubacterium sp. strain VPI 12708 g...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Mallonee DH,Hylemon PB

    更新日期:1999-02-01 00:00:00

  • Truncated Ubiquitin 5' Regulatory Region from Erianthus arundinaceus Drives Enhanced Transgene Expression in Heterologous Systems.

    abstract::Characterization of novel plant gene promoters underpins the development of transgenic crop plants. Here, we report a novel 5' regulatory sequence (Eriubi D7) of the ubiquitin gene from Erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. A 3.2-kb regulatory sequence of u...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Chakravarthi M,Philip A,Subramonian N

    更新日期:2015-09-01 00:00:00

  • T-DNA transfer to maize plants.

    abstract::Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on ...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Shen WH,Escudero J,Hohn B

    更新日期:1999-12-01 00:00:00

  • Expression of human rotavirus chimeric fusion proteins from replicating but non disseminating adenovectors and elicitation of rotavirus-specific immune responses in mice.

    abstract::The aim of this study was to evaluate the usefulness of replicating but non disseminating adenovirus vectors (AdVs) as vaccine vector using human rotavirus (HRV) as a model pathogen. HRV VP7, VP4, or VP4Δ (N-terminal 336 amino acids of VP4) structural proteins as well as the VP4Δ::VP7 chimeric fusion protein were expr...

    journal_title:Molecular biotechnology

    pub_type: 杂志文章


    authors: Girard A,Roques E,St-Louis MC,Massie B,Archambault D

    更新日期:2013-07-01 00:00:00