Abstract：
:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a complex macromolecule, namely an antibody. When applied to the heavy and light chain genes of our model antibody, we found that greater numbers of high-producing transfectants as well as increased levels of protein production were observed (approximately 1.5-fold). In this test model, production was improved even though the antibody has previously been demonstrated to give high expression in stably transfected cells (up to 5 g/L in bioreactors). Because the parental heavy chain sequence contained introns, and the process of gene optimization is most efficiently performed on sequences without introns, we demonstrated that removal of introns in the coding sequence had no effect on the quantity of antibody produced. All constructs were evaluated using Lonza's glutamine synthetase gene expression vectors in Chinese hamster ovary cells. Our findings suggest that significant improvements in product yields can be achieved by gene optimization, which may facilitate the processing and translation of gene transcripts.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Kalwy S,Rance J,Young Rdoi
10.1385/MB:34:2:151subject
Has Abstractpub_date
2006-10-01 00:00:00pages
151-6issue
2eissn
1073-6085issn
1559-0305pii
MB:34:2:151journal_volume
34pub_type
杂志文章abstract：:It is difficult to obtain precise and accurate estimates of the true mycotoxin concentration of a bulk lot when using a mycotoxin-sampling plan that measures the concentration in a small portion of the bulk lot. A mycotoxin-sampling plan is defined by a mycotoxin test procedure and a defined accept/reject limit. A myc...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:23:1:61
更新日期：2003-01-01 00:00:00
abstract：:A single-stage polymerase-based procedure is described that allows extensive modifications of DNA. The version described here uses the QuikChange Site-Directed Mutagenesis System kit supplied by Stratagene. The original protocol is replaced by a single-stage method in which linear production of complementary strands i...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:29:3:225
更新日期：2005-03-01 00:00:00
abstract：:Biobanks, more formally known as biological resource centers (BRCs), form an "unsung" yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and cell-lines would be effectively impossible. BRCs...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
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更新日期：2008-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789331
更新日期：1995-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9291-4
更新日期：2010-11-01 00:00:00
abstract：:The mapping of genetic loci within organisms has been accelerated by the advent of Radiation Hybrid (RH) panels. These panels are available for humans and non-humans including mice, baboon, rat, and canine. This article contains a general protocol for the use of the Genebridge 4 whole genome RH panel to map a human lo...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:19:2:205
更新日期：2001-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:20:3:315
更新日期：2002-03-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:19:1:045
更新日期：2001-09-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0080-9
更新日期：2018-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12033-015-9843-8
更新日期：2015-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:23:2:171
更新日期：2003-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-9997-7
更新日期：2017-05-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9260-y
更新日期：2010-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:20:2:137
更新日期：2002-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:17:1:43
更新日期：2001-01-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9750-4
更新日期：2014-08-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0140-1
更新日期：2019-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9555-2
更新日期：2013-06-01 00:00:00
abstract：:Hereditary cancers account for approximately 10 % of breast and ovarian cancers. Mutations of the BRCA1 and BRCA2 genes, encoding two proteins involved in DNA repair, underlie most cases of such hereditary cancers. Women with BRCA mutations develop breast cancer in 50-80 % of cases and ovarian cancer in 10-40 % of cas...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-012-9646-0
更新日期：2013-07-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9830-5
更新日期：2015-05-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9731-7
更新日期：2014-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
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更新日期：2008-03-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9101-4
更新日期：2009-02-01 00:00:00
abstract：:In the context of clinical gene transfer using viral vectors, the risk of memory antivector immunity is often poorly appreciated. The immunological past of the patient, the site of injection, and the vector dose will play intertwined and decisive roles in the safety and efficacy of treatment. To circumvent the drawbac...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:34:2:247
更新日期：2006-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02752254
更新日期：1997-10-01 00:00:00
abstract：:The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of d...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9182-8
更新日期：2009-10-01 00:00:00
abstract：:RNA editing is one of the post-transcriptional RNA processes. RNA editing generates RNA and protein diversity in eukaryotes and results in specific amino acid substitutions, deletions, and changes in gene expression levels. Adenosine-to-inosine RNA editing represents the most important class of editing in human and af...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-012-9498-7
更新日期：2012-09-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:15:3:243
更新日期：2000-07-01 00:00:00
abstract：:The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:24:3:229
更新日期：2003-07-01 00:00:00
abstract：:Internal ribosome entry site (IRES) elements are highly structured RNA sequences that function to recruit ribosomes for the initiation of translation. In contrast to the canonical cap-binding, the mechanism of IRES-mediated translation initiation is still poorly understood. Translation initiation of the coxsackievirus...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-013-9674-4
更新日期：2013-10-01 00:00:00
