Abstract：
:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a complex macromolecule, namely an antibody. When applied to the heavy and light chain genes of our model antibody, we found that greater numbers of high-producing transfectants as well as increased levels of protein production were observed (approximately 1.5-fold). In this test model, production was improved even though the antibody has previously been demonstrated to give high expression in stably transfected cells (up to 5 g/L in bioreactors). Because the parental heavy chain sequence contained introns, and the process of gene optimization is most efficiently performed on sequences without introns, we demonstrated that removal of introns in the coding sequence had no effect on the quantity of antibody produced. All constructs were evaluated using Lonza's glutamine synthetase gene expression vectors in Chinese hamster ovary cells. Our findings suggest that significant improvements in product yields can be achieved by gene optimization, which may facilitate the processing and translation of gene transcripts.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Kalwy S,Rance J,Young Rdoi
10.1385/MB:34:2:151subject
Has Abstractpub_date
2006-10-01 00:00:00pages
151-6issue
2eissn
1073-6085issn
1559-0305pii
MB:34:2:151journal_volume
34pub_type
杂志文章abstract：:The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:24:3:229
更新日期：2003-07-01 00:00:00
abstract：:Tumor necrosis factor (TNF) is a central cytokine in the pathogenesis of septic shock and other inflammatory states. Assay by immunoassay is convenient, but, because of circulating soluble receptors, does not accurately reflect biological activity of the cytokine. This article describes how to perform a bioassay for T...
journal_title：Molecular biotechnology
pub_type： 杂志文章
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:29:1:57
更新日期：2005-01-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:21:2:105
更新日期：2002-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9144-1
更新日期：2009-06-01 00:00:00
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journal_title：Molecular biotechnology
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0065-8
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:12:3:237
更新日期：1999-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9756-y
更新日期：2014-08-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:13:1:29
更新日期：1999-11-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:14:3:197
更新日期：2000-03-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:31:1:001
更新日期：2005-09-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:30:2:103
更新日期：2005-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-0003-7
更新日期：2007-06-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:12:2:137
更新日期：1999-09-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02907471
更新日期：1995-08-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:20:2:137
更新日期：2002-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9659-3
更新日期：2013-10-01 00:00:00
abstract：:The rhoptry 2 protein (Rop2) is an interesting protein of Toxoplasma gondii that is involved in the parasite invasion of host cell, it has three T-cell epitopes and high antigenic value. However, the expression of Rop2 as a recombinant protein in Escherichia coli is not an easy task, showing low levels of expression o...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:18:3:269
更新日期：2001-07-01 00:00:00
abstract：:In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9429-z
更新日期：2012-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9799-0
更新日期：2015-01-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9078-z
更新日期：2008-11-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0021-z
更新日期：2017-08-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-011-9395-5
更新日期：2011-10-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02789066
更新日期：1996-04-01 00:00:00
abstract：:Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0140-1
更新日期：2019-02-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00245-z
更新日期：2020-04-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9231-3
更新日期：2010-03-01 00:00:00
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journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02745880
更新日期：1994-12-01 00:00:00
abstract：:Papaya ringspot virus (PRSV) causes severe economic losses in both cucurbits and papaya throughout the tropics and subtropics. Development of PRSV-resistant transgenic plants faces a major hurdle in achieving resistance against geographically distinct isolates. One of the major reasons of failing to achieve the broad-...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9205-5
更新日期：2010-01-01 00:00:00
