Rapid bovine and caprine species identification in ruminant feeds by duplex real-time PCR melting curve analysis using EvaGreen fluorescence dye.

abstract：:RNA editing is one of the post-transcriptional RNA processes. RNA editing generates RNA and protein diversity in eukaryotes and results in specific amino acid substitutions, deletions, and changes in gene expression levels. Adenosine-to-inosine RNA editing represents the most important class of editing in human and af...

journal_title：Molecular biotechnology

authors： Tang W,Fei Y,Page M

更新日期：2012-09-01 00:00:00

abstract：:The significance of actin cytoskeleton on cell growth was historically studied using toxic drugs, such as cytochalasin. However, it is possible that unpredictable effects of these agents may have influenced the reported observations. In our study, we have established a drug-free system using cofilin overexpression to ...

journal_title：Molecular biotechnology

authors： Lee YJ,Keng PC

更新日期：2005-09-01 00:00:00

abstract：:The gram-negative anaerobic oral bacterium Porphyromonas gingivalis initiates periodontal disease through fimbrial attachment to saliva-coated oral surfaces. To study the effects of immunomodulation on enhancement of subunit vaccination, the expression in E. coli and immunogenicity of P. gingivalis fimbrial protein (F...

journal_title：Molecular biotechnology

authors： Kim TG,Huy NX,Kim MY,Jeong DK,Jang YS,Yang MS,Langridge WH,Lee JY

更新日期：2009-02-01 00:00:00

abstract：:To develop combined vaccine systems, we have generated Edwardsiella tarda ghosts (ETG) displaying a foreign protein on the outer membrane and also Ed. tarda cadaver (ETC) expressing a heterologous protein in the cytoplasm. Green fluorescent protein (GFP) was used as a model foreign protein. A constitutive promoter (Et...

journal_title：Molecular biotechnology

authors： Choi SH,Nam YK,Kim KH

更新日期：2010-10-01 00:00:00

abstract：:A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...

journal_title：Molecular biotechnology

authors： Wang TT,Cheng WC,Lee BH

更新日期：1998-10-01 00:00:00

abstract：:Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representi...

journal_title：Molecular biotechnology

authors： Noble JE,Knight AE,Reason AJ,Di Matola A,Bailey MJ

更新日期：2007-10-01 00:00:00

abstract：:A functional assay for proteolytic processing of the amyloid precursor protein (APP) was set up in yeast. This consisted of a membrane-bound chimeric protein containing the beta-secretase cleaved C-terminal fragment of APP fused to the Ga14 transcription factor. Using this chimera in a GAL-reporter yeast strain, an ex...

journal_title：Molecular biotechnology

authors： Gunyuzlu PL,White WH,Davis GL,Hollis GF,Toyn JH

更新日期：2000-05-01 00:00:00

abstract：:Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last y...

journal_title：Molecular biotechnology

authors： Thiele A,Stangl GI,Schutkowski M

更新日期：2011-11-01 00:00:00

abstract：:This article presents a simple and rapid method for removal of unincorporated label and proteins from DNA sequencing reactions by using Wizard purification resin. This method can be successfully applied for preparation of end-labeled oligonucleotides free of unincorporated label, which is important in experiments (inc...

journal_title：Molecular biotechnology

authors： Kaczorowski T,Sektas M

更新日期：1996-04-01 00:00:00

abstract：:IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalyti...

journal_title：Molecular biotechnology

authors： Mitsuda Y,Planque S,Hara M,Kyle R,Taguchi H,Nishiyama Y,Paul S

更新日期：2007-06-01 00:00:00

abstract：:Yeast artificial chromosome (YAC) cloning systems enable the cloning of DNA stretches of 50 to well over 2000 kb. This makes it possible to study large intact regions of DNA in detail, by restriction mapping the YAC to produce a physical map and by examining the YAC for coding sequences or genes. YACs are important fo...

journal_title：Molecular biotechnology

authors： Ramsay M

更新日期：1994-04-01 00:00:00

abstract：:The rhoptry 2 protein (Rop2) is an interesting protein of Toxoplasma gondii that is involved in the parasite invasion of host cell, it has three T-cell epitopes and high antigenic value. However, the expression of Rop2 as a recombinant protein in Escherichia coli is not an easy task, showing low levels of expression o...

journal_title：Molecular biotechnology

authors： Nigro M,Martin V,Kaufer F,Carral L,Angel SO,Pszenny V

更新日期：2001-07-01 00:00:00

abstract：:Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic analyses of the gut of C. curvignathus revealed...

journal_title：Molecular biotechnology

authors： Woon JS,King PJH,Mackeen MM,Mahadi NM,Wan Seman WMK,Broughton WJ,Abdul Murad AM,Abu Bakar FD

更新日期：2017-07-01 00:00:00

abstract：:Transgenic rice seed can be utilized as a bioreactor to produce high-value recombinant proteins. Mouse interleukin 4 (mIL-4) and mIL-6 were specifically expressed as secretory proteins in rice endosperm by ligating the N-terminal glutelin B-1 (GluB-1) signal peptide and the C-terminal KDEL endoplasmic reticulum retent...

journal_title：Molecular biotechnology

authors： Fujiwara Y,Yang L,Takaiwa F,Sekikawa K

更新日期：2016-04-01 00:00:00

abstract：:Since it was first introduced in late 1990s Wave bioreactor has been used for protein production by mammalian and insect cell lines. However, using Wave bioreactor to produce human monoclonal antibody by stable Drosophila Schneider 2 (S2) cell transfectants has not been reported before. In this study, S2 cells were co...

journal_title：Molecular biotechnology

authors： Wang L,Hu H,Yang J,Wang F,Kaisermayer C,Zhou P

更新日期：2012-10-01 00:00:00

abstract：:Characterization of novel plant gene promoters underpins the development of transgenic crop plants. Here, we report a novel 5' regulatory sequence (Eriubi D7) of the ubiquitin gene from Erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. A 3.2-kb regulatory sequence of u...

journal_title：Molecular biotechnology

authors： Chakravarthi M,Philip A,Subramonian N

更新日期：2015-09-01 00:00:00

abstract：:Brn-3a, a member of the POU gene family (so-called because of the similarity with the group of transcription factors Pit, Oct, and Unc), was found in neuronal cells engaged in the transcription activity of the p1 and p2 promoters of the most powerful antiapoptotic gene, namely, Bcl-2. The alternative splicing of Brn-3...

journal_title：Molecular biotechnology

authors： Chiarugi V,Del Rosso M,Magnelli L

更新日期：2002-10-01 00:00:00

abstract：:Tall fescue plants are widely exposed to white grubs. Regarding the rate of damage caused by the white grubs to tall fescue and difficulty of its ecological and economical control, production of resistant cultivars is a priority. In this experiment, for the first time, we report production of transgenic lines resistan...

journal_title：Molecular biotechnology

authors： Hosseini HR,Salehi H,Alichi M

更新日期：2019-07-01 00:00:00

abstract：:The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organ...

journal_title：Molecular biotechnology

authors： Gatter M,Gatter T,Matthäus F

更新日期：2012-10-01 00:00:00

abstract：:RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21-23 nucleotides synthesized in vitro or expressed by DNA-based vector. However, introduction of siRNA into mammalian cells by transfection limits the application of RNAi, especiall...

journal_title：Molecular biotechnology

authors： Zhao N,Zu ZX,Liu CM,Dong WJ,Liu DP,Liang CC

更新日期：2004-11-01 00:00:00

abstract：:Previous studies have shown that mRNA-electroporated dendritic cells (DCs) are able to process and present tumor-associated antigens, leading to the activation of tumor-specific T cells in vitro and in vivo. However, the optimal maturation state of antigen loading and half-life of the mRNA-translated protein product a...

journal_title：Molecular biotechnology

authors： Met O,Eriksen J,Svane IM

更新日期：2008-10-01 00:00:00

abstract：:Efficient display of antibody on filamentous phage M13 coat is crucial for successful biopanning selections. We applied a directed evolution strategy to improve the oligovalent display of a poorly behaving Fab fragment fused to phage gene-3 for minor coat protein (g3p). The Fab displaying clones were enriched from a r...

journal_title：Molecular biotechnology

authors： Huovinen T,Sanmark H,Ylä-Pelto J,Vehniäinen M,Lamminmäki U

更新日期：2010-03-01 00:00:00

abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...

journal_title：Molecular biotechnology

authors： Rotman G,Vanagaite L,Collins FS,Shiloh Y

更新日期：1995-04-01 00:00:00

abstract：:The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of d...

journal_title：Molecular biotechnology

authors： Thanh T,Omar H,Abdullah MP,Chi VT,Noroozi M,Ky H,Napis S

更新日期：2009-10-01 00:00:00

abstract：:The induction of several secondary metabolites in plants is one of the most commonly observed effects after the external addition of methyl jasmonate (MeJA). After the elicitation of Catharanthus roseus hairy roots with different concentrations of MeJA, changes in the accumulation of alkaloids such as ajmalicine, serp...

journal_title：Molecular biotechnology

authors： Ruiz-May E,Galaz-Avalos RM,Loyola-Vargas VM

更新日期：2009-03-01 00:00:00

abstract：:Hereditary cancers account for approximately 10 % of breast and ovarian cancers. Mutations of the BRCA1 and BRCA2 genes, encoding two proteins involved in DNA repair, underlie most cases of such hereditary cancers. Women with BRCA mutations develop breast cancer in 50-80 % of cases and ovarian cancer in 10-40 % of cas...

journal_title：Molecular biotechnology

authors： Vuttariello E,Borra M,Calise C,Mauriello E,Greggi S,Vecchione A,Biffali E,Chiappetta G

更新日期：2013-07-01 00:00:00

abstract：:A single-stage polymerase-based procedure is described that allows extensive modifications of DNA. The version described here uses the QuikChange Site-Directed Mutagenesis System kit supplied by Stratagene. The original protocol is replaced by a single-stage method in which linear production of complementary strands i...

journal_title：Molecular biotechnology

authors： Salerno JC,Jones RJ,Erdogan E,Smith SM

更新日期：2005-03-01 00:00:00

abstract：:Classic toxicology studies often utilize in vivo animal models. Newer approaches employing in vitro organ-specific cellular models have been developed in recent years to help accelerate the speed and reduce the cost of traditional toxicology testing. Toward the goal of supporting in vitro cellular model research with ...

journal_title：Molecular biotechnology

authors： Mossoba ME,Vohra SN,Bigley E 3rd,Sprando J,Wiesenfeld PL

更新日期：2020-04-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...

journal_title：Molecular biotechnology

authors： Hristodorov D,Fischer R,Joerissen H,Müller-Tiemann B,Apeler H,Linden L

更新日期：2013-03-01 00:00:00

abstract：:Genes are recognized as undergoing genomic imprinting when they are capable of being expressed only from the paternal or only from the maternal chromosome. The process can occur coordinately within large physical domains in mammalian chromosomes. One interesting facet of the study of genomic imprinting is that it offe...

journal_title：Molecular biotechnology

authors： Greally JM

更新日期：1999-04-01 00:00:00