Abstract:
:RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21-23 nucleotides synthesized in vitro or expressed by DNA-based vector. However, introduction of siRNA into mammalian cells by transfection limits the application of RNAi, especially when it is necessary to generate long-term gene silencing in vivo. Virus vector-mediated RNAi provides an alternative to transfection. In the present study, we investigated such transduction system and showed that retrovirus vector-mediated RNAi can substantially down-regulate expression of mouse adult beta-globin gene in MEL cells. The results suggest that retrovirus vector-delivered RNAi may find its use in functional genomics and in gene therapy.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Zhao N,Zu ZX,Liu CM,Dong WJ,Liu DP,Liang CCdoi
10.1385/MB:28:3:195keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
195-9issue
3eissn
1073-6085issn
1559-0305pii
MB:28:3:195journal_volume
28pub_type
杂志文章abstract::To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most pro...
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pub_type: 杂志文章
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