Abstract:
:The concept of photoprotection by dietary means is gaining momentum. Plant constituents such as carotenoids and flavonoids are involved in protection against excess light in plants and contribute to the prevention of UV damage in humans. As micronutrients, they are ingested with the diet and are distributed into light-exposed tissues, such as skin or the eye where they provide systemic photoprotection. beta-Carotene and lycopene prevent UV-induced erythema formation. Likewise, dietary flavanols exhibit photoprotection. After about 10-12 weeks of dietary intervention, a decrease in the sensitivity toward UV-induced erythema was observed in volunteers. Dietary micronutrients may contribute to life-long protection against harmful UV radiation.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Stahl W,Sies Hdoi
10.1007/s12033-007-0051-zsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
26-30issue
1eissn
1073-6085issn
1559-0305pii
MB:37:1:26journal_volume
37pub_type
杂志文章,评审abstract::In the context of clinical gene transfer using viral vectors, the risk of memory antivector immunity is often poorly appreciated. The immunological past of the patient, the site of injection, and the vector dose will play intertwined and decisive roles in the safety and efficacy of treatment. To circumvent the drawbac...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:34:2:247
更新日期:2006-10-01 00:00:00
abstract::Enterotoxigenic strains of Escherichia coli produce a heat-labile holotoxin (LT), which causes diarrhea. We engineered corn seeds to produce LT-B, the nontoxic subunit of LT, to serve as a plant-derived vaccine to traveler's diarrhea and as an adjuvant for co-administered proteins. We previously demonstrated that a st...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:32:1:031
更新日期:2006-01-01 00:00:00
abstract::Any biopharmaceutical product that has involved the use of animal-derived material during the manufacturing process has the potential to be contaminated with animal viruses. To ensure safety of these products, extensive testing is performed on the starting materials, such as the cell banks, and on the raw materials us...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:21:1:057
更新日期:2002-05-01 00:00:00
abstract::The synthesis of complete genes is becoming a more and more popular approach in heterologous gene expression. Reasons for this are the decreasing prices and the numerous advantages in comparison to classic molecular cloning methods. Two of these advantages are the possibility to adapt the codon usage to the host organ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9479-2
更新日期:2012-10-01 00:00:00
abstract::DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:20:3:315
更新日期:2002-03-01 00:00:00
abstract::The low economic profits of feather recycling lead that the large amount of feathers is currently discarded in China. To convert feather hydrolysates into GSH with high values, surface display of the bifunctional glutathione synthetase encoded by gcsgs from Streptococcus thermophilus on Saccharomyces cerevisiae and th...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9750-4
更新日期:2014-08-01 00:00:00
abstract::Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:20:2:137
更新日期:2002-02-01 00:00:00
abstract::NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expans...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00250-2
更新日期:2020-05-01 00:00:00
abstract::Internal ribosome entry site (IRES) elements are highly structured RNA sequences that function to recruit ribosomes for the initiation of translation. In contrast to the canonical cap-binding, the mechanism of IRES-mediated translation initiation is still poorly understood. Translation initiation of the coxsackievirus...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-013-9674-4
更新日期:2013-10-01 00:00:00
abstract::DNA damage is one of the most impactful events in living organisms, leading to DNA sequence changes (mutation) and disruption of biological processes. A study has identified a protein called Damage Suppressor Protein (Dsup) in the tardigrade Ramazzotius varieornatus that has shown to reduce the effects of radiation da...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00273-9
更新日期:2020-12-01 00:00:00
abstract::Many preclinical studies have shown RNA interference (RNAi) as a new promising way to treat various human diseases including cancer and virus infection and there is an increasing demand for the large-scale preparation of short interfering RNAs (siRNAs) at low cost. Data are accumulating to show that endoribonuclease-p...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:31:3:203
更新日期:2005-11-01 00:00:00
abstract::In this study, we investigated the relative expression of the Rous sarcoma virus (RSV) promoter-driven expression of enhanced green fluorescent protein (EGFP) in fibroblasts of transgenic quails. We analyzed the direct influence of CpG methylation of the RSV promoter on the transcriptional activity of delivered transg...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9393-7
更新日期:2011-11-01 00:00:00
abstract::L-Cysteine desulfhydrase (DES; EC 4.4.1.1) is the most important enzyme that catalyzes the decomposition of L-cysteine to pyruvate, ammonia, and hydrogen sulfide (H2S), the latter of which has recently been recognized as the third gasotransmitter for multiple signaling events in plants. Previous results showed the exi...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9621-9
更新日期:2013-07-01 00:00:00
abstract::Menstrual blood has been recognized as an easily accessible and inexpensive source of stem cells, in recent years. To establish a safe and efficient protocol for development of menstrual blood-derived stem cells (MenSCs) into osteoblasts, the effect of substitution of fetal bovine serum (FBS) with human platelet deriv...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-013-9698-9
更新日期:2014-03-01 00:00:00
abstract::Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achieveme...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-018-0133-0
更新日期:2019-01-01 00:00:00
abstract::Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has be...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0065-8
更新日期:2018-03-01 00:00:00
abstract::Epoxide hydrolases (EHs; 3.3.2.x) catalyze the enantioselective ring opening of racemic epoxides to the corresponding enantiopure vicinal diols and remaining equivalent unreacted epoxides. These epoxides and diols are used for the synthesis of chiral drug intermediates. With an upsurge in the methods for identificatio...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-017-9996-8
更新日期:2017-03-01 00:00:00
abstract::Hypermethylated in Cancer-1 (HIC-1) is a novel tumor suppressor plays crucial role in tumor formation through loss of function by hypermethylation. HIC-1 is known as transcriptional factor whereas little known about its structure and function. Requirement felt to clone and express full coding protein and reveal variou...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9783-8
更新日期:2014-11-01 00:00:00
abstract::An endochitinase gene from the Serratia marcescens Nima strain (chiA Nima) was cloned, sequenced, and expressed in Escherichia coli DH5alphaF', and the recombinant protein (ChiA Nima) was purified by hydrophobic interaction chromatography. chiA Nima contains an open reading frame (ORF) that encodes an endochitinase wi...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:31:2:103
更新日期:2005-10-01 00:00:00
abstract::Terminal Restriction Fragment Length Polymorphism (T-RFLP) or Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (FluRFLP) have made a significant impact on the way in which PCR products amplified from mixed community DNA extracts have been assessed. Technically, these approaches are essent...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:16:3:261
更新日期:2000-11-01 00:00:00
abstract::In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00194-2
更新日期:2019-09-01 00:00:00
abstract::Yeast artificial chromosome (YAC) cloning systems enable the cloning of DNA stretches of 50 to well over 2000 kb. This makes it possible to study large intact regions of DNA in detail, by restriction mapping the YAC to produce a physical map and by examining the YAC for coding sequences or genes. YACs are important fo...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02921558
更新日期:1994-04-01 00:00:00
abstract::Host protein synthesis is shut down in the lytic baculovirus expression vector system (BEVS). This also affects host proteins involved in routing secretory proteins through the endoplasmic reticulum (ER)-Golgi system. It has been demonstrated that a secretory alkaline phosphatase-EGFP fusion protein (SEFP) can act as ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9545-4
更新日期:2013-05-01 00:00:00
abstract::Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:14:1:01
更新日期:2000-01-01 00:00:00
abstract::The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02761713
更新日期:1996-12-01 00:00:00
abstract::A short (43-bp) A/T-rich stretch of DNA located in the intergenic region between the baiA2 and baiF genes from Eubacterium sp. strain VPI 12708 was amplified by polymerase chain reaction (PCR) and inserted in front of the Shine-Dalgarno (SD) sequences of three inefficiently-expressed Eubacterium sp. strain VPI 12708 g...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789174
更新日期:1999-02-01 00:00:00
abstract::Recombinant proteins are useful tools in biological research, drug development, and drug screening. Specially designed expression vectors have been developed to introduce cDNA for recombinant protein expression in mammalian cells. We have combined a dicistronic mRNA design for expression of the recombinant protein, us...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02745860
更新日期:1998-08-01 00:00:00
abstract::A sensitive method for staining proteins after transfer from polyacrylamide gels to nitrocellulose paper is described. Transferred proteins are first derivatized by reaction of the nitrocellulose replica with sulfosuccinimidobiotin and are then reacted sequentially with streptavidin, rabbit anti-streptavidin, and hors...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:15:2:161
更新日期:2000-06-01 00:00:00
abstract::PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-016-9932-3
更新日期:2016-05-01 00:00:00
abstract::Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last y...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-011-9402-x
更新日期:2011-11-01 00:00:00