Abstract:
:DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation. Sequence comparisons of the two available DNA beta sequences has identified a highly conserved region upstream of a predicted hairpin structure. Abutting primers designed to this conserved region allows PCR-mediated amplification of the full-length DNA beta component from total nucleic acid extracts isolated from infected plants originating from a variety of geographically distinct sources and host plants.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Briddon RW,Bull SE,Mansoor S,Amin I,Markham PGdoi
10.1385/MB:20:3:315keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
315-8issue
3eissn
1073-6085issn
1559-0305pii
MB:20:3:315journal_volume
20pub_type
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