Rapid establishment of high-producing cell lines using dicistronic vectors with glutamine synthetase as the selection marker.

abstract：:Gene reporter systems play a key role in gene expression and regulation studies. This review describes the ideal reporter systems, including reporter expression vector design. It summarizes the many uses of genetic reporters and outlines the currently available and commonly used reporter systems. Each system is descri...

journal_title：Molecular biotechnology

authors： Schenborn E,Groskreutz D

更新日期：1999-11-01 00:00:00

abstract：:Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...

journal_title：Molecular biotechnology

authors： Djurovic S,Iversen N,Jeansson S,Hoover F,Christensen G

更新日期：2004-09-01 00:00:00

abstract：:The ability to identify disease-associated genes using microarray technology is dependent on isolating high-quality total RNA from the diseased tissue under study. However, it is not always possible to obtain large amounts of affected tissue from patients and therefore the quality and yield of RNA may be compromised. ...

journal_title：Molecular biotechnology

authors： Murray EJ

更新日期：2003-02-01 00:00:00

abstract：:Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches h...

journal_title：Molecular biotechnology

authors： Morokuma D,Xu J,Hino M,Mon H,Merzaban JS,Takahashi M,Kusakabe T,Lee JM

更新日期：2017-05-01 00:00:00

abstract：:D-Stereospecific amidohydrolase (DAH) from Streptomyces sp. 82F2 has potential utility for the synthesis of D/L configuration dipeptides by an aminolysis reaction. Structural comparison of DAH with substrate-bound D-amino acid amidase revealed that three residues located in the active site pocket of DAH (Thr145, Ala26...

journal_title：Molecular biotechnology

authors： Elyas YYA,Miyatani K,Shimizu K,Arima J

更新日期：2018-09-01 00:00:00

abstract：:Characterization of novel plant gene promoters underpins the development of transgenic crop plants. Here, we report a novel 5' regulatory sequence (Eriubi D7) of the ubiquitin gene from Erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. A 3.2-kb regulatory sequence of u...

journal_title：Molecular biotechnology

authors： Chakravarthi M,Philip A,Subramonian N

更新日期：2015-09-01 00:00:00

abstract：:Modern sugarcane cultivars have complex genetic characteristics and low fertility that render their genetic improvement through traditional breeding difficult. Genetic engineering methodology to introduce foreign genes provides new opportunities for the genetic improvement of sugarcane cultivars. One of prerequisites ...

journal_title：Molecular biotechnology

authors： Santosa DA,Hendroko R,Farouk A,Greiner R

更新日期：2004-10-01 00:00:00

abstract：:Lactococcus lactis is industrially important microorganism used in many dairy fermentations. Numerous genes and gene expression signals from this organism have now been identified and characterized. Recently, several naturally occurring, inducible gene-expression systems have also been described in L. lactis. The main...

journal_title：Molecular biotechnology

authors： Djordjevic GM,Klaenhammer TR

更新日期：1998-04-01 00:00:00

abstract：:This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be den...

journal_title：Molecular biotechnology

authors： Molloy PE,Graham BM,Cupit PM,Grant SD,Porter AJ,Cunningham C

更新日期：1995-12-01 00:00:00

abstract：:We have previously described a microarray platform combining live basophils with protein arrays suitable for high-throughput detection of sensitisation against allergens. During optimisation of this technique, we observed severe losses of adhering cells during the washing steps, particularly after activation. In order...

journal_title：Molecular biotechnology

authors： Wang X,Cato P,Lin HC,Li T,Wan D,Alcocer MJ,Falcone FH

更新日期：2014-02-01 00:00:00

abstract：:An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could repr...

journal_title：Molecular biotechnology

authors： Wang Z,Wang HY,Feng H

更新日期：2013-01-01 00:00:00

abstract：:In an effort to develop microalgae as a robust system for the production of valuable proteins, we analyzed some of the factors affecting recombinant protein expression in the chloroplast of the green alga Chlamydomonas reinhardtii. We monitored mRNA accumulation, protein synthesis, and protein turnover for three codon...

journal_title：Molecular biotechnology

authors： Coragliotti AT,Beligni MV,Franklin SE,Mayfield SP

更新日期：2011-05-01 00:00:00

abstract：:The use of synthetic genes may constitute a successful approach for the heterologous production and functional expression of bacterial antimicrobial peptides (bacteriocins) by recombinant yeasts. In this work, synthetic genes with adapted codon usage designed from the mature amino acid sequence of the bacteriocin ente...

journal_title：Molecular biotechnology

authors： Jiménez JJ,Borrero J,Gútiez L,Arbulu S,Herranz C,Cintas LM,Hernández PE

更新日期：2014-06-01 00:00:00

abstract：:The induction of several secondary metabolites in plants is one of the most commonly observed effects after the external addition of methyl jasmonate (MeJA). After the elicitation of Catharanthus roseus hairy roots with different concentrations of MeJA, changes in the accumulation of alkaloids such as ajmalicine, serp...

journal_title：Molecular biotechnology

authors： Ruiz-May E,Galaz-Avalos RM,Loyola-Vargas VM

更新日期：2009-03-01 00:00:00

abstract：:The aim of this study was to evaluate the usefulness of replicating but non disseminating adenovirus vectors (AdVs) as vaccine vector using human rotavirus (HRV) as a model pathogen. HRV VP7, VP4, or VP4Δ (N-terminal 336 amino acids of VP4) structural proteins as well as the VP4Δ::VP7 chimeric fusion protein were expr...

journal_title：Molecular biotechnology

authors： Girard A,Roques E,St-Louis MC,Massie B,Archambault D

更新日期：2013-07-01 00:00:00

abstract：:Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant geno...

journal_title：Molecular biotechnology

authors： Zvirin T,Magrisso L,Yaari A,Shoseyov O

更新日期：2018-06-01 00:00:00

abstract：:Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last y...

journal_title：Molecular biotechnology

authors： Thiele A,Stangl GI,Schutkowski M

更新日期：2011-11-01 00:00:00

abstract：:Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study, a thermostable pectin methylesterase (CtPME) from Clostridium thermocellum belonging to family 8 carbohydrate...

journal_title：Molecular biotechnology

authors： Rajulapati V,Goyal A

更新日期：2017-05-01 00:00:00

abstract：:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...

journal_title：Molecular biotechnology

authors： Kalwy S,Rance J,Young R

更新日期：2006-10-01 00:00:00

abstract：:Addition of taxol into suspension cultures of Taxus cuspidata induced cell apoptosis, which was confirmed by gel electrophoresis of the DNA ladders indicating the progressive delineation of fragmented nuclear DNA (nDNA) into distinct bodies. The additional taxol not only changed the microtubule assembly of cells, but ...

journal_title：Molecular biotechnology

authors： Yuan YJ,Ma ZY,Wu JC

更新日期：2002-02-01 00:00:00

abstract：:Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: int...

journal_title：Molecular biotechnology

authors： Cichorz S,Gośka M,Litwiniec A

更新日期：2014-10-01 00:00:00

abstract：:This article describes the construction, expression, and purification of RNase single-chain antibody fusion proteins. To construct a fusion protein, the gene for each moiety, the RNase and the binding ligand, is modified separately to contain complementary DNA encoding a 13 amino acid spacer that separates the RNase f...

journal_title：Molecular biotechnology

authors： Newton DL,Rybak SM

更新日期：2002-01-01 00:00:00

abstract：:De novo design provides an in silico toolkit for the design of novel small molecular structures to a set of specified structural constraints. With the avalanche of bioinformatics data, de novo design is ideally suited for exploring molecules that could be useful for chemical genomics. The design process involves manip...

journal_title：Molecular biotechnology

authors： Dean PM

更新日期：2007-11-01 00:00:00

abstract：:The two asparagine-linked glycosylation sites of recombinant coagulation factor VIIa have been characterized by glycosidase digestions, size-exclusion chromatography (SEC), and mass spectrometry (MS). Nine structures were characterized as core fucosylated bi-and triantennary structures with 0-3 sialic-acid residues wh...

journal_title：Molecular biotechnology

authors： Klausen NK,Bayne S,Palm L

更新日期：1998-06-01 00:00:00

abstract：:Efficient display of antibody on filamentous phage M13 coat is crucial for successful biopanning selections. We applied a directed evolution strategy to improve the oligovalent display of a poorly behaving Fab fragment fused to phage gene-3 for minor coat protein (g3p). The Fab displaying clones were enriched from a r...

journal_title：Molecular biotechnology

authors： Huovinen T,Sanmark H,Ylä-Pelto J,Vehniäinen M,Lamminmäki U

更新日期：2010-03-01 00:00:00

abstract：:We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...

journal_title：Molecular biotechnology

authors： Todorov T,Todorova A,Georgieva B,Mitev V

更新日期：2010-06-01 00:00:00

abstract：:Brn-3a, a member of the POU gene family (so-called because of the similarity with the group of transcription factors Pit, Oct, and Unc), was found in neuronal cells engaged in the transcription activity of the p1 and p2 promoters of the most powerful antiapoptotic gene, namely, Bcl-2. The alternative splicing of Brn-3...

journal_title：Molecular biotechnology

authors： Chiarugi V,Del Rosso M,Magnelli L

更新日期：2002-10-01 00:00:00

abstract：:Biobanks, more formally known as biological resource centers (BRCs), form an "unsung" yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and cell-lines would be effectively impossible. BRCs...

journal_title：Molecular biotechnology

authors： Day JG,Stacey GN

更新日期：2008-10-01 00:00:00

abstract：:An endochitinase gene from the Serratia marcescens Nima strain (chiA Nima) was cloned, sequenced, and expressed in Escherichia coli DH5alphaF', and the recombinant protein (ChiA Nima) was purified by hydrophobic interaction chromatography. chiA Nima contains an open reading frame (ORF) that encodes an endochitinase wi...

journal_title：Molecular biotechnology

authors： Ruiz-Sanchez A,Cruz-Camarillo R,Salcedo-Hernandez R,Ibarra JE,Barboza-Corona JE

更新日期：2005-10-01 00:00:00

abstract：:Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technol...

journal_title：Molecular biotechnology

authors： McCarthy JJ

更新日期：2003-11-01 00:00:00