Stable Expression of Adalimumab in Nicotiana tabacum.

Abstract:

:Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant genome offers a clear advantage in agricultural protein production platforms, limited only by the number of hectares that can be cultivated. We report here, for the first time, successful and stable expression of adalimumab in transgenic Nicotiana tabacum plants. The plant-derived adalimumab proved fully active and was shown to rescue L929 cells from the in vitro lethal effect of rhTNFα just as effectively as commercially available CHO-derived adalimumab (Humira). These results indicate that agricultural biopharming is an efficient alternative to mammalian cell-based expression platforms for the large-scale production of recombinant antibodies.

journal_name

Mol Biotechnol

journal_title

Molecular biotechnology

authors

Zvirin T,Magrisso L,Yaari A,Shoseyov O

doi

10.1007/s12033-018-0075-6

subject

Has Abstract

pub_date

2018-06-01 00:00:00

pages

387-395

issue

6

eissn

1073-6085

issn

1559-0305

pii

10.1007/s12033-018-0075-6

journal_volume

60

pub_type

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