Reporter gene vectors and assays.

abstract：:Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...

journal_title：Molecular biotechnology

authors： Rotman G,Vanagaite L,Collins FS,Shiloh Y

更新日期：1995-04-01 00:00:00

abstract：:In this study, we investigated the relative expression of the Rous sarcoma virus (RSV) promoter-driven expression of enhanced green fluorescent protein (EGFP) in fibroblasts of transgenic quails. We analyzed the direct influence of CpG methylation of the RSV promoter on the transcriptional activity of delivered transg...

journal_title：Molecular biotechnology

authors： Jang HJ,Choi JW,Kim YM,Shin SS,Lee K,Han JY

更新日期：2011-11-01 00:00:00

abstract：:The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of d...

journal_title：Molecular biotechnology

authors： Thanh T,Omar H,Abdullah MP,Chi VT,Noroozi M,Ky H,Napis S

更新日期：2009-10-01 00:00:00

abstract：:N-Succinyl-amino acid racemase (NSAAR), long referred to as N-acyl- or N-acetyl-amino acid racemase, is an enolase superfamily member whose biotechnological potential was discovered decades ago, due to its use in the industrial dynamic kinetic resolution methodology first known as "Acylase Process". In previous works,...

journal_title：Molecular biotechnology

authors： Soriano-Maldonado P,Andújar-Sánchez M,Clemente-Jiménez JM,Rodríguez-Vico F,Las Heras-Vázquez FJ,Martínez-Rodríguez S

更新日期：2015-05-01 00:00:00

abstract：:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...

journal_title：Molecular biotechnology

authors： Ryu KH,Choi SH,Lee JS

更新日期：2000-01-01 00:00:00

abstract：:The simple use of nonisotopic hybridization probes to detect complementary sequences provides valuable information in a large number of research and commercial applications. In hybridization assays, the four "S's (speed, simplicity, sensitivity, and specificity) are important criteria for determining the choice of pro...

journal_title：Molecular biotechnology

authors： Ruth JL

更新日期：1996-10-01 00:00:00

abstract：:Plants synthesize amino acids by collateral metabolic pathways using primary elements carbon and oxygen from air, hydrogen from water in soil and nitrogen from soil. Following synthesis, amino acids are immediately used for metabolism, transient storage or transported to the phloem. Different families of transporters ...

journal_title：Molecular biotechnology

authors： Chakraborty N,Besra A,Basak J

更新日期：2020-03-01 00:00:00

abstract：:Production of monoclonal antibodies and pharmaceutical proteins in transgenic plants has been the focus of many research efforts for close to 30 years. Use of plants as bioreactors reduces large-scale production costs and minimizes risk for human pathogens contamination. Stable nuclear transformation of the plant geno...

journal_title：Molecular biotechnology

authors： Zvirin T,Magrisso L,Yaari A,Shoseyov O

更新日期：2018-06-01 00:00:00

abstract：:Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...

journal_title：Molecular biotechnology

authors： Novo C,Domingos A,Karmali A

更新日期：2001-02-01 00:00:00

abstract：:Adipose tissue-derived mesenchymal stromal cells (ADSCs) are a prominent cellular source for regenerative medicine. We tested whether transplantation of ADSCs into the ischemic muscular tissue of diabetic animals would attenuate impaired cell metabolism and microcirculatory function. We induced unilateral hind limb is...

journal_title：Molecular biotechnology

authors： Madonna R,Delli Pizzi S,Tartaro A,De Caterina R

更新日期：2014-05-01 00:00:00

abstract：:Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow ...

journal_title：Molecular biotechnology

authors： Graham FL,Prevec L

更新日期：1995-06-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...

journal_title：Molecular biotechnology

authors： Hristodorov D,Fischer R,Joerissen H,Müller-Tiemann B,Apeler H,Linden L

更新日期：2013-03-01 00:00:00

abstract：:An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained b...

journal_title：Molecular biotechnology

authors： Weber K,Bolander ME,Sarkar G

更新日期：1998-02-01 00:00:00

abstract：:PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...

journal_title：Molecular biotechnology

authors： Lorenzo V,Mascanzoni F,Vitagliano L,Ruvo M,Doti N

更新日期：2016-05-01 00:00:00

abstract：:Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last y...

journal_title：Molecular biotechnology

authors： Thiele A,Stangl GI,Schutkowski M

更新日期：2011-11-01 00:00:00

abstract：:Human immunodeficiency virus types 1 and 2 (HIV-1, -2), the etiological agents of AIDS, are retroviruses that replicate in CD4+ T lymphocytes, monocytes, and macrophages. Early anti-HIV therapies were directed at the step in the virus life cycle that was considered the most readily blocked: the transcription of viral ...

journal_title：Molecular biotechnology

authors： Patience C,Moore J,Boyd M

更新日期：1994-02-01 00:00:00

abstract：:The enzyme avian myeloblastosis virus reverse transcriptase (AMV-RT) is routinely used for cDNA synthesis, which is generally carried out at temperatures between 37 degrees C and 42 degrees C. We show that this enzyme can support cDNA synthesis, at temperatures as high as 70 degrees C. We have utilized this property o...

journal_title：Molecular biotechnology

authors： Fuchs B,Zhang K,Rock MG,Bolander ME,Sarkar G

更新日期：1999-10-01 00:00:00

abstract：:Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...

journal_title：Molecular biotechnology

authors： Pegoraro M,Matić S,Pergolizzi B,Iannarelli L,Rossi AM,Morra M,Noris E

更新日期：2017-10-01 00:00:00

abstract：:Human adipose tissue-derived stromal cells (ADSCs) are being evaluated for cardiovascular repair. We developed an ex vivo method for producing angiogenic ADSCs transduced with a self-inactivating lentiviral vector (LV) expressing the enhanced green fluorescence protein (EGFP) from an internal cytomegalovirus (CMV) pro...

journal_title：Molecular biotechnology

authors： Madonna R,Bolli R,Rokosh G,De Caterina R

更新日期：2013-05-01 00:00:00

abstract：:RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21-23 nucleotides synthesized in vitro or expressed by DNA-based vector. However, introduction of siRNA into mammalian cells by transfection limits the application of RNAi, especiall...

journal_title：Molecular biotechnology

authors： Zhao N,Zu ZX,Liu CM,Dong WJ,Liu DP,Liang CC

更新日期：2004-11-01 00:00:00

abstract：:DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation...

journal_title：Molecular biotechnology

authors： Briddon RW,Bull SE,Mansoor S,Amin I,Markham PG

更新日期：2002-03-01 00:00:00

abstract：:D-Allulose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity. Using D-psicose epimerase and L-rhamnulose kinase, a method enabling high conversio...

journal_title：Molecular biotechnology

authors： Xiao Q,Niu J,Liu H,Liu Y,Zhou X

更新日期：2019-06-01 00:00:00

abstract：:DNA damage is one of the most impactful events in living organisms, leading to DNA sequence changes (mutation) and disruption of biological processes. A study has identified a protein called Damage Suppressor Protein (Dsup) in the tardigrade Ramazzotius varieornatus that has shown to reduce the effects of radiation da...

journal_title：Molecular biotechnology

authors： Kirke J,Jin XL,Zhang XH

更新日期：2020-12-01 00:00:00

abstract：:The advent of recombinant antibody technology led to an enormous revival in the use of antibodies as diagnostic and therapeutic tools for fighting cancer. This review provides a brief historical sketch of the development of recombinant antibodies for the diagnosis and immunotherapy of cancer and summarizes the most si...

journal_title：Molecular biotechnology

authors： Krauss J

更新日期：2003-09-01 00:00:00

abstract：:The biosensor field has grown enormously since the first demonstration of the biosensor concept by Leland C. Clark, Jr. in 1962. Today's biosensor market is dominated by glucose biosensors, mass-produced enzyme electrodes for the rapid self-diagnosis of blood glucose levels by diabetes sufferers. Here we take a histor...

journal_title：Molecular biotechnology

authors： Newman JD,Setford SJ

更新日期：2006-03-01 00:00:00

abstract：:Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...

journal_title：Molecular biotechnology

authors： Yang Y,Wang Q,Guo D

更新日期：2008-09-01 00:00:00

abstract：:This article reviews some of the issues involved in evaluating vaccines in humans. Vaccine trials are required for licensure and are essential for demonstrating a vaccine's safety and protective efficacy. The formal framework of phase I, II, and III trials is described, with particular emphasis on the choice of hypoth...

journal_title：Molecular biotechnology

authors： Farrington CP,Miller E

更新日期：2001-01-01 00:00:00

abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...

journal_title：Molecular biotechnology

authors： do Nascimento NC,Menguer PK,Sperotto RA,de Almeida MR,Fett-Neto AG

更新日期：2013-05-01 00:00:00

abstract：:Association studies using genome scans to identify quantitative trait loci for multifactorial disorders, with anything approaching reasonable power, have been compromised by the need for a very dense array of genetic markers and large numbers of affected individuals. These requirements impose enormous burdens on the g...

journal_title：Molecular biotechnology

authors： Curran S,Hill L,O'Grady G,Turic D,Asherson P,Taylor E,Sham P,Craig I,Vaughan P

更新日期：2002-11-01 00:00:00

abstract：:Lentivirus-mediated RNA interference (RNAi) is a potent experimental tool for investigating gene functions in vitro and in vivo. It has advantages that transgenic technology lacks. However, in vivo applications are difficult to apply in the central nervous system of non-model organisms due to the lack of a standard br...

journal_title：Molecular biotechnology

authors： Chen Q,Zhu T,Jones G,Zhang J,Sun Y

更新日期：2013-06-01 00:00:00