Brn-3a, a neuronal transcription factor of the POU gene family: indications for its involvement in cancer and angiogenesis.

abstract：:Hypermethylated in Cancer-1 (HIC-1) is a novel tumor suppressor plays crucial role in tumor formation through loss of function by hypermethylation. HIC-1 is known as transcriptional factor whereas little known about its structure and function. Requirement felt to clone and express full coding protein and reveal variou...

journal_title：Molecular biotechnology

authors： Kumar S

更新日期：2014-11-01 00:00:00

abstract：:Expression of certain transgenes from an adenovirus vector can be deleterious to its own replication. This can result in the inhibition of virus rescue, reduced viral yields, or, in the worst case, make it impossible to construct a vector expressing the inhibiting transgene product. A gene regulation system based on t...

journal_title：Molecular biotechnology

authors： Gall JG,Lizonova A,EttyReddy D,McVey D,Zuber M,Kovesdi I,Aughtman B,King CR,Brough DE

更新日期：2007-03-01 00:00:00

abstract：:Recombinant proteins are useful tools in biological research, drug development, and drug screening. Specially designed expression vectors have been developed to introduce cDNA for recombinant protein expression in mammalian cells. We have combined a dicistronic mRNA design for expression of the recombinant protein, us...

journal_title：Molecular biotechnology

authors： Pu H,Cashion LM,Kretschmer PJ,Liu Z

更新日期：1998-08-01 00:00:00

abstract：:A shuttle vector pZL1 which can replicate both in Gluconobacter oxydans and Escherichia coli was constructed based on G. oxydans DSM2003 cryptic plasmid pGOX3, a homology of G. oxydans 621H pGOX3, and E. coli cloning vector pUC18. It was found to be stably maintained in G. oxydans during the serial subcultures in the ...

journal_title：Molecular biotechnology

authors： Zhang L,Lin J,Ma Y,Wei D,Sun M

更新日期：2010-11-01 00:00:00

abstract：:A single-stage polymerase-based procedure is described that allows extensive modifications of DNA. The version described here uses the QuikChange Site-Directed Mutagenesis System kit supplied by Stratagene. The original protocol is replaced by a single-stage method in which linear production of complementary strands i...

journal_title：Molecular biotechnology

authors： Salerno JC,Jones RJ,Erdogan E,Smith SM

更新日期：2005-03-01 00:00:00

abstract：:In this study, we investigated the relative expression of the Rous sarcoma virus (RSV) promoter-driven expression of enhanced green fluorescent protein (EGFP) in fibroblasts of transgenic quails. We analyzed the direct influence of CpG methylation of the RSV promoter on the transcriptional activity of delivered transg...

journal_title：Molecular biotechnology

authors： Jang HJ,Choi JW,Kim YM,Shin SS,Lee K,Han JY

更新日期：2011-11-01 00:00:00

abstract：:Immobilized-metal-ion affinity chromatography (IMAC) is used extensively for phosphopeptide enrichment in phosphoproteomics. However, the effect of nucleic acids in protein samples on phosphopeptide enrichment by IMAC has not yet been well clarified. In this study, we demonstrate that IMAC beads possess a strong adsor...

journal_title：Molecular biotechnology

authors： Li Y,Luo Y,Wu S,Gao Y,Liu Y,Zheng D

更新日期：2009-09-01 00:00:00

abstract：:Brucellosis, one of the most important re-emerging zoonoses in many countries, is caused by bacteria belonging to the genus Brucella. Furthermore these bacteria represent potential biological warfare agents and the identification of species and biovars of field strains may be crucial for tracing back source of infecti...

journal_title：Molecular biotechnology

authors： De Santis R,Ancora M,De Massis F,Ciammaruconi A,Zilli K,Di Giannatale E,Pittiglio V,Fillo S,Lista F

更新日期：2013-10-01 00:00:00

abstract：:Indirubin is a strong inhibitor of several eukaryotic cell signaling pathways and shows promise as a treatment for myelocytic leukemia and Alzheimer's disease. The tmoABCDEF operon, encoding the components of a novel toluene 4-monooxygenase from the paint factory soil isolate, Pseudomonas sp. M4, was cloned and expres...

journal_title：Molecular biotechnology

authors： Wongsaroj L,Sallabhan R,Dubbs JM,Mongkolsuk S,Loprasert S

更新日期：2015-08-01 00:00:00

abstract：:Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to oth...

journal_title：Molecular biotechnology

authors： Japelaghi RH,Haddad R,Garoosi GA

更新日期：2011-10-01 00:00:00

abstract：:Costimulatory signal(s) in addition to engagement of T cell receptor is important for optimal activation of T lymphocytes. During last decade several dozens of new costimulatory molecules, including 4-1BB, CD40, OX40, CD27, and ICOS, have been identified. It is likely that in Post-Genome Era more molecules with potent...

journal_title：Molecular biotechnology

authors： Chapoval AI,Zhu G,Chen L

更新日期：2002-07-01 00:00:00

abstract：:Molecular biological methods that use antibodies and nucleic acids to detect specific foodborne bacterial pathogens were scarcely known a decade and a half ago. Few scientists could have predicted that these tools of basic research would come to dominate the field of food diagnostics. Today, a large number of cleverly...

journal_title：Molecular biotechnology

authors： Feng P

更新日期：1997-06-01 00:00:00

abstract：:Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood ...

journal_title：Molecular biotechnology

authors： Morse MA,Clay T,Colling K,Lyerly HK

更新日期：2003-09-01 00:00:00

abstract：:Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxy...

journal_title：Molecular biotechnology

authors： Pegoraro M,Matić S,Pergolizzi B,Iannarelli L,Rossi AM,Morra M,Noris E

更新日期：2017-10-01 00:00:00

abstract：:The gram-negative anaerobic oral bacterium Porphyromonas gingivalis initiates periodontal disease through fimbrial attachment to saliva-coated oral surfaces. To study the effects of immunomodulation on enhancement of subunit vaccination, the expression in E. coli and immunogenicity of P. gingivalis fimbrial protein (F...

journal_title：Molecular biotechnology

authors： Kim TG,Huy NX,Kim MY,Jeong DK,Jang YS,Yang MS,Langridge WH,Lee JY

更新日期：2009-02-01 00:00:00

abstract：:Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow ...

journal_title：Molecular biotechnology

authors： Graham FL,Prevec L

更新日期：1995-06-01 00:00:00

abstract：:Lentiviral transduction is a highly efficient DNA delivery method for RNA interference applications. However, obtaining high lentiviral titers of shRNA and miRNA encoding vectors is challenging, since shRNA and miRNA cassettes have been shown to reduce lentiviral titers. In this study, we compare four commercially ava...

journal_title：Molecular biotechnology

authors： Albrecht C,Hosiner S,Tichy B,Aldrian S,Hajdu S,Nürnberger S

更新日期：2015-06-01 00:00:00

abstract：:The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes needed for efficient solubilization of native cellulose, the major component of plants. In this work, we reported the molecular characterization of an important glycosyl-hydrolase enzyme classified as endo-β-1,4-glucanase. ...

journal_title：Molecular biotechnology

authors： Chahed H,Ezzine A,Mlouka AB,Hardouin J,Jouenne T,Marzouki MN

更新日期：2014-04-01 00:00:00

abstract：:This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia...

journal_title：Molecular biotechnology

authors： Meneghetti S,Costacurta A,Frare E,Da Rold G,Migliaro D,Morreale G,Crespan M,Sotés V,Calò A

更新日期：2011-07-01 00:00:00

abstract：:Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...

journal_title：Molecular biotechnology

authors： Tülek A,Karataş E,Çakar MM,Aydın D,Yılmazcan Ö,Binay B

更新日期：2021-01-01 00:00:00

abstract：:IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalyti...

journal_title：Molecular biotechnology

authors： Mitsuda Y,Planque S,Hara M,Kyle R,Taguchi H,Nishiyama Y,Paul S

更新日期：2007-06-01 00:00:00

abstract：:The ability to identify disease-associated genes using microarray technology is dependent on isolating high-quality total RNA from the diseased tissue under study. However, it is not always possible to obtain large amounts of affected tissue from patients and therefore the quality and yield of RNA may be compromised. ...

journal_title：Molecular biotechnology

authors： Murray EJ

更新日期：2003-02-01 00:00:00

abstract：:The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of d...

journal_title：Molecular biotechnology

authors： Thanh T,Omar H,Abdullah MP,Chi VT,Noroozi M,Ky H,Napis S

更新日期：2009-10-01 00:00:00

abstract：:Tea, a beverage crop, is a rich source of polyphenols and polysaccharides which greatly attribute to its importance. However, oxidation and precipitation of these compounds during nucleic acids extraction is a limitation to molecular biology and genomic studies. On isolation of total RNA from root tissue using establi...

journal_title：Molecular biotechnology

authors： Muoki RC,Paul A,Kumari A,Singh K,Kumar S

更新日期：2012-09-01 00:00:00

abstract：:In this study, we investigated expression and dimerization of an ER-associated degradation (ERAD) substrate, a null Hong Kong variant of α-1-antitrypsin (NHK) using immunoblotting assay and a novel NanoLuc complementary reporter system called the NanoBiT (NB) assay. This NB-tagged NHK made it possible to monitor the i...

journal_title：Molecular biotechnology

authors： Norisada J,Fujimura K,Amaya F,Kohno H,Hirata Y,Oh-Hashi K

更新日期：2018-08-01 00:00:00

abstract：:We have modified the differential display of 3'-end restriction fragments of cDNA technique by combining it with the amplified fragment length polymorphism (AFLP) approach and silver staining. Modified oligo-dT primers were used for a reverse transcription step. ds cDNA was digested with the Mse I restriction enzyme a...

journal_title：Molecular biotechnology

authors： Ivashuta S,Imai R,Uchiyama K,Gau M

更新日期：1999-09-01 00:00:00

abstract：:Sample preparation is crucial for obtaining high-quality proteins for the purpose of electrophoretic separation and further analysis from tissues that contain high levels of interfering compounds. Hypericum perforatum is a medicinal plant that contains high amounts of phenolic compounds, of which hypericins, hyperfori...

journal_title：Molecular biotechnology

authors： Karppinen K,Taulavuori E,Hohtola A

更新日期：2010-11-01 00:00:00

abstract：:We report on a unified rapid betaine-based-PCR protocol for amplification of the (CAG)n region in Huntington disease (HD) and the (CGG)n region in Fragile X syndrome (FXS), followed by an electrophoretic separation on automated sequencer for precise determination of the triplet numbers. The high betaine concentration ...

journal_title：Molecular biotechnology

authors： Todorov T,Todorova A,Georgieva B,Mitev V

更新日期：2010-06-01 00:00:00

abstract：:Lactic acid bacteria (LAB) are generally sensitive to hydrogen peroxide (H(2)O(2)), Lactobacillus sakei YSI8 is one of the very few LAB strains able to degrade H(2)O(2) through the action of a heme-dependent catalase. Lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product ferment...

journal_title：Molecular biotechnology

authors： An H,Zhou H,Huang Y,Wang G,Luan C,Mou J,Luo Y,Hao Y

更新日期：2010-06-01 00:00:00

abstract：:UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indol...

journal_title：Molecular biotechnology

authors： do Nascimento NC,Menguer PK,Sperotto RA,de Almeida MR,Fett-Neto AG

更新日期：2013-05-01 00:00:00