Abstract:
:Brucellosis, one of the most important re-emerging zoonoses in many countries, is caused by bacteria belonging to the genus Brucella. Furthermore these bacteria represent potential biological warfare agents and the identification of species and biovars of field strains may be crucial for tracing back source of infection, allowing to discriminate naturally occurring outbreaks instead of bioterrorist events. In the last years, multiple-locus variable-number tandem repeat analysis (MLVA) has been proposed as complement of the classical biotyping methods and it has been applied for genotyping large collections of Brucella spp. At present, the MLVA band profiles may be resolved by automated or manual procedures. The Lab on a chip technology represents a valid alternative to standard genotyping techniques (as agarose gel electrophoresis) and it has been previously used for Brucella genotyping. Recently, a new high-throughput genotyping analysis system based on capillary gel electrophoresis, the QIAxcel, has been described. The aim of the study was to evaluate the ability of two DNA sizing equipments, the QIAxcel System and the Lab chip GX, to correctly call alleles at the sixteen loci including one frequently used MLVA assay for Brucella genotyping. The results confirmed that these technologies represent a meaningful advancement in high-throughput Brucella genotyping. Considering the accuracy required to confidently resolve loci discrimination, QIAxcel shows a better ability to measure VNTR allele sizes compared to LabChip GX.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
De Santis R,Ancora M,De Massis F,Ciammaruconi A,Zilli K,Di Giannatale E,Pittiglio V,Fillo S,Lista Fdoi
10.1007/s12033-013-9659-3subject
Has Abstractpub_date
2013-10-01 00:00:00pages
101-10issue
2eissn
1073-6085issn
1559-0305journal_volume
55pub_type
杂志文章abstract::Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02740841
更新日期:1998-12-01 00:00:00
abstract::Small ubiquitin-related modifier (SUMO) fusion system has been shown to be efficient for enhancing expression and preventing degradation of the target protein. We showed herein that SUMO fusion to human keratinocyte growth factor 2 (hKGF-2) gene was feasible and it significantly enhanced protein expression and its eff...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9135-7
更新日期:2009-05-01 00:00:00
abstract::Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:25:1:95
更新日期:2003-09-01 00:00:00
abstract::Expression of certain transgenes from an adenovirus vector can be deleterious to its own replication. This can result in the inhibition of virus rescue, reduced viral yields, or, in the worst case, make it impossible to construct a vector expressing the inhibiting transgene product. A gene regulation system based on t...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02686012
更新日期:2007-03-01 00:00:00
abstract::In this study, we investigated the relative expression of the Rous sarcoma virus (RSV) promoter-driven expression of enhanced green fluorescent protein (EGFP) in fibroblasts of transgenic quails. We analyzed the direct influence of CpG methylation of the RSV promoter on the transcriptional activity of delivered transg...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-011-9393-7
更新日期:2011-11-01 00:00:00
abstract::The use of synthetic genes may constitute a successful approach for the heterologous production and functional expression of bacterial antimicrobial peptides (bacteriocins) by recombinant yeasts. In this work, synthetic genes with adapted codon usage designed from the mature amino acid sequence of the bacteriocin ente...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9731-7
更新日期:2014-06-01 00:00:00
abstract::A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789291
更新日期:1994-08-01 00:00:00
abstract::Cyclooxygenase (COX)-2 plays an important role in the development of cancer and has been recognized as a potential therapeutic target. Because nonsteroidal anti-inflammatory drugs (NSAIDs) are able to inhibit the activity of this enzyme, the potential efficacy of such drugs for purposes of cancer prevention or therapy...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:34:3:329
更新日期:2006-11-01 00:00:00
abstract::Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achieveme...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-018-0133-0
更新日期:2019-01-01 00:00:00
abstract::A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02760858
更新日期:1998-10-01 00:00:00
abstract::Monoclonal antibodies (Mabs) against human chorionic gonadotropin hormone (hCG) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. Sixty-five percent of the total culture wells exhibited hybrid growth and 8% of the total wells (13 culture wells) contained anti-hCG secreting hybrids. A pos...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:17:2:119
更新日期:2001-02-01 00:00:00
abstract::Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attra...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-018-0140-1
更新日期:2019-02-01 00:00:00
abstract::There is a growing pharmaceutical market for protein-based drugs for use in therapy and diagnosis. The rapid developments in molecular and cell biology have resulted in production of expression systems for manufacturing of recombinant proteins and monoclonal antibodies. These proteins are glycosylated when expressed i...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02745880
更新日期:1994-12-01 00:00:00
abstract::Derivation of cardiomyocytes directly from patients' own fibroblasts could offer a new therapeutic approach for those with ischemic heart disease. An essential step toward clinical application is to establish safe conversion of human fibroblasts into a cardiac fate. Here we aimed to efficiently and safely generate car...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-017-0007-x
更新日期:2017-06-01 00:00:00
abstract::This review outlines the various methods available for submitting sequence data to the EMBL Nucleotide Sequence Database. Depending on the type of sequence data and the facilities available to the submitter, one method may be more suitable than another. Recent developments have been the World Wide Web submission tool,...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02762322
更新日期:1996-08-01 00:00:00
abstract::Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadv...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:17:3:219
更新日期:2001-03-01 00:00:00
abstract::DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:20:3:315
更新日期:2002-03-01 00:00:00
abstract::The diversity and complexity of bioinformatics tools currently available for protein sequence analysis can make it difficult to know where to begin when presented with a new sequence. In this article, we present a protocol outlining one approach to sequence analysis that should give as comprehensive a picture as possi...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-007-0032-2
更新日期:2007-07-01 00:00:00
abstract::Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in th...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9066-3
更新日期:2008-09-01 00:00:00
abstract::Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:28:1:21
更新日期:2004-09-01 00:00:00
abstract::The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of d...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-009-9182-8
更新日期:2009-10-01 00:00:00
abstract::To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most pro...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-008-9054-7
更新日期:2008-06-01 00:00:00
abstract::The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those s...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1385/MB:29:1:57
更新日期:2005-01-01 00:00:00
abstract::Human papillomavirus (HPV) is the causal agent of cervical cancer, one of the most common causes of death in women worldwide, and its E7 antigen is the major candidate for a therapeutic vaccine. The large scale production of E7 by molecular farming that would lead to the development of a safe and inexpensive vaccine i...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9781-x
更新日期:2014-11-01 00:00:00
abstract::D-Allulose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity. Using D-psicose epimerase and L-rhamnulose kinase, a method enabling high conversio...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-019-00174-6
更新日期:2019-06-01 00:00:00
abstract::Internal ribosome entry site (IRES) elements are highly structured RNA sequences that function to recruit ribosomes for the initiation of translation. In contrast to the canonical cap-binding, the mechanism of IRES-mediated translation initiation is still poorly understood. Translation initiation of the coxsackievirus...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12033-013-9674-4
更新日期:2013-10-01 00:00:00
abstract::Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1385/MB:13:2:165
更新日期:1999-12-01 00:00:00
abstract::Monoclonal antibodies (mAbs) are the fastest growing class of biopharmaceuticals reflecting their diverse applications in research and the clinic. The correct glycosylation of mAbs is required to elicit effector functions such as complement-dependent and antibody-dependent cell-mediated cytotoxicity, although these ma...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-012-9531-x
更新日期:2013-03-01 00:00:00
abstract::Laccases are polyphenol oxidoreductases used in a number of industrial applications. Due to the increasing demand for these "green catalysis" enzymes, the identification and biochemical characterisation of their novel properties is essential. In our study, cloned Madurella mycetomatis laccase (mmlac) genes were hetero...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-020-00281-9
更新日期:2021-01-01 00:00:00
abstract::The low economic profits of feather recycling lead that the large amount of feathers is currently discarded in China. To convert feather hydrolysates into GSH with high values, surface display of the bifunctional glutathione synthetase encoded by gcsgs from Streptococcus thermophilus on Saccharomyces cerevisiae and th...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/s12033-014-9750-4
更新日期:2014-08-01 00:00:00